Dataset: Water_Column_AE1409
Deployment: AE1409

Water column data from cruise AE1409

Principal Investigator:

Benjamin A.S. Van Mooy (Woods Hole Oceanographic Institution, WHOI)

Co-Principal Investigator:

Sonya T. Dyhrman (Lamont-Doherty Earth Observatory, LDEO)

BCO-DMO Data Manager:

Mathew Biddle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation (P Processing by Tricho)

Current State:

Final no updates expected

Deployment Synonyms:

PABST

Data URL:

https://www.bco-dmo.org/dataset-deployment/709483/data

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Description

Water column data from cruise AE1409.

Methods & Sampling

Dataset acquisition description

MAGIC Soluble reactive phosphorus (SRP) concentrations. SRP (i.e. phosphate) was determined in seawaters samples and incubations was quantified using MAGnesium Induced Coprecipitation (MAGIC) as described by Karl and Tien (1992).

Total dissolved phosphorus (TDP) concentrations – TDP was determined on 0.2 um filtrates of surface water (~5 m depth) samples collected via CTD into acid-clean polycarbonate bottles. Samples were processed at the SOEST Laboratory for Analytical Biogeochemistry at the University of Hawaii, according to facility protocols.

Alkaline phosphatase rates - Alkaline phosphatase activity samples were obtained by placing 2-5 cleaned Trichodesmium colonies on 5 um PC filters, gently vacuum filtering away excess liquid, then storing in 47 mm plastic petri dishes at -20 C until analysis. Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT)

Data Processing Description

Dataset Processing Description

BCO-DMO Processing:
Added conventional header with dataset name, PI name, version date.
Modified parameter names to conform with BCO-DMO naming conventions.
Re-formatted date from m/dd/yyyy to yyyymmdd.
Replaced spaces and / with underscores.
Added coordinate information to the data from the station list provided by the PI.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1332898	NSF Division of Ocean Sciences
OCE-1332912	NSF Division of Ocean Sciences

Instruments

Niskin bottle

Supplied Name: Niskin bottles

Supplied Description:

Water samples for whole community analyses were collected from Niskin bottles deployed on a rosette with a CTD.

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Nutrient Autoanalyzer

Supplied Name: AA3 Nutrient Autoanalyzer

Supplied Description:

Samples were processed at the SOEST Laboratory for Analytical Biogeochemistry at the University of Hawaii and analyzed for phosphate via the AA3 autoanalyzer.

More information about the Phosphorous measurements can be found at the SOEST Laboratory Webiste.

Instrument Type

Generic Name: Nutrient Autoanalyzer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/

Generic Description:

Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples.

plate reader

Supplied Name: Synergy H1 Hybrid plate reader

Supplied Description:

Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT).

Reference:

Dyhrman, S.T., Ruttenberg, K.C., 2006. Presence and regulation of alkaline phosphatase activity in eukaryotic phytoplankton from the coastal ocean: Implications for dissolved organic phosphorus remineralization,. Limnol. Oceanogr. 51, 1381-1390.

Instrument Type

Generic Name: plate reader

Generic Description:

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.

Water Temperature Sensor

Supplied Name: temperature logger (Onset)

Supplied Description:

Temperature in the incubators was occasionally monitored with a waterproof temperature logger (Onset).

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Sampling date formatted as YYYYMMDD.	YYYYMMDD	date
Station	Numeric identifier for the station where the data was collected.	unitless	sta
Depth	Depth at which the sample was collected.	meters	depth
Sample	Numeric identifier of the sample.	unitless	sample
SRP	Soluble reactive phosphorous (SRP).	micromole per liter (umol/L)	SRP
MAGIC_SRP	soluble reactive phosphorus measured by magnesium-induced co-precipitation	micromole per liter (umol/L)	SRP
TDP	Total dissolved phosphorus.	micromole per liter (umol/L)	TDP
DOP_calc	Dissolved organic phosphorus.	micromole per liter (umol/L)	DOP
Lat	Latitude of sampling. Positive indicate North.	Decimal Degrees	lat
Long	Longitude of sampling. Negative indicate West.	Decimal Degrees	lon

Database

Contribute Data

Dataset: Water_Column_AE1409
Deployment: AE1409

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Water_Column_AE1409Deployment: AE1409

Dataset acquisition description

Dataset Processing Description

Dataset: Water_Column_AE1409
Deployment: AE1409