Dataset: Chesapeake Bay: Ammonia oxidation rates
Deployment: HRS1317

Principal Investigator:

Alyson E. Santoro (University of California-Santa Barbara, UCSB)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: New Approaches to New Production (N-SPOT)

Gene content, gene expression, and physiology in mesopelagic ammonia-oxidizing archaea (AmoA Archaea)

Deployment Synonyms:

1302

Data URL:

https://www.bco-dmo.org/dataset-deployment/738603/data

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Description

Related Datasets (also generated from samples during cruises HRS1316 and HRS1317):
* Chesapeake Bay: Nitrous oxide https://www.bco-dmo.org/dataset/738594
* Chesapeake Bay: N2 to Ar ratio https://www.bco-dmo.org/dataset/738604
* Chesapeake Bay: Nitrate https://www.bco-dmo.org/dataset/738609
* Chesapeake Bay: Nitrite https://www.bco-dmo.org/dataset/738614
* Chesapeake Bay: Ammonium https://www.bco-dmo.org/dataset/738619

The results paper for these data has been submitted to the journal Estuaries and Coasts (Laperriere et al., submitted).

Methods & Sampling

Dataset acquisition description

Water samples were collected using a 12 x 10 L Niskin bottle rosette sampler equipped with a conductivity, temperature, and pressure instrument package (SBE9, Sea-Bird Electronics, Bellevue, Washington, U.S.A.) and a sensor for dissolved oxygen (SBE43, Sea-Bird).

Water for ammonia oxidation rate incubations was collected directly from the rosette into 250 mL polycarbonate bottles.

Rates of water column ammonia oxidation were measured from bottle incubations with 15NH4Cl (Dugdale and Goering 1967; Sigman et al. 2001; Damashek et al. 2016). Isotope samples were analyzed at the University of California Davis Stable Isotope Facility or the Central Appalachians Stable Isotope Facility at the University of Maryland Center for Environmental Science with a ThermoFinnigan GasBench + PreCon trace gas concentration system interfaced to a ThermoScientific Delta V Plus isotope-ratio mass spectrometer.

Data Processing Description

Dataset Processing Description

BCO-DMO Data Manager Processing Notes:
* added a conventional header with dataset name, PI name, version date
* modified parameter names to conform with BCO-DMO naming conventions
* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.
* removed spaces between minutes and seconds
* removed trailing and leading spaces of values
* removed occasional space after decimal place in original longitude decimal degree format
* added timestamp in UTC (ISO format)

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1260006	NSF Division of Ocean Sciences
OCE-1437310	NSF Division of Ocean Sciences
OCE-1740538	NSF Division of Ocean Sciences

Instruments

CTD Sea-Bird 9

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: CTD Sea-Bird 9

Acronym: CTD SBE 9

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/130/

Generic Description:

The Sea-Bird SBE 9 is a type of CTD instrument package. The SBE 9 is the Underwater Unit and is most often combined with the SBE 11 Deck Unit (for real-time readout using conductive wire) when deployed from a research vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorometer, altimeter, etc.). Note that in most cases, it is more accurate to specify SBE 911 than SBE 9 since it is likely a SBE 11 deck unit was used. more information from Sea-Bird Electronics

Isotope-ratio Mass Spectrometer

Supplied Name: ThermoScientific Delta V Plus isotope-ratio mass spectrometer

Supplied Description:

ThermoFinnigan GasBench + PreCon trace gas concentration system interfaced to a ThermoScientific Delta V Plus isotope-ratio mass spectrometer

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Niskin bottle

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Niskin bottle

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/

Generic Description:

A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
station	Station number	unitless	station
date	Date of sample collection (local EDT)	unitless	date
time	Time of sample collection (local EDT)	untiless	time
lat	Latitude (N)	degrees decimal minutes	lat
lon	Longitude (W)	degrees decimal minutes	lon
depth	Depth	meters	depth
amm_ox	Ammonia oxidation rate	nanomoles per liter per hour (nmol/L/h)	NH4
replicate	Replicate number	unitless	replicate
ISO_DateTime_UTC	ISO timestamp based on the ISO 8601:2004(E) standard in format YYYY-mm-ddTHH:MMZ (UTC)	unitless	ISO_DateTime_UTC
lat_dd	Latitude	decimal degrees	lat
lon_dd	Longitude	decimal degrees	lon

Database

Contribute Data

Dataset: Chesapeake Bay: Ammonia oxidation rates
Deployment: HRS1317

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Chesapeake Bay: Ammonia oxidation ratesDeployment: HRS1317

Dataset acquisition description

Dataset Processing Description

Dataset: Chesapeake Bay: Ammonia oxidation rates
Deployment: HRS1317