Dataset: OlyGrowth_Expt_2018_Chemistry

Name: Daily water chemistry measurements from a larval growth experiment culturing Olympia oysters in 50 unique combinations of temperature, salinity, and pCO2 over up to 17 days of larval life.
Published: 2019-09-05
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.776260.1

Temporal Extent: 2018-05-04 - 2018-05-21

Project:

RUI: Will climate change cause 'lazy larvae'? Effects of climate stressors on larval behavior and dispersal (Climate stressors on larvae)

Principal Investigator:

Shawn M. Arellano (Western Washington University, WWU)

Co-Principal Investigator:

M. Brady Olson (Western Washington University, WWU)

Sylvia Yang (Western Washington University, WWU)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2019-09-05

Restricted:

Release Date:

2020-08-31

Validated:

Yes

Current State:

Final no updates expected

Daily water chemistry measurements from a larval growth experiment culturing Olympia oysters in 50 unique combinations of temperature, salinity, and pCO2 over up to 17 days of larval life.

Abstract:

Daily water chemistry measurements from a larval growth experiment culturing Olympia oysters in 50 unique combinations of temperature, salinity, and pCO2 over up to 17 days of larval life.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2019-09-05	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.776260.1	2020-08-31

Description

Daily water chemistry measurements from a larval growth experiment culturing Olympia oysters in 50 unique combinations of temperature, salinity, and pCO2 over up to 17 days of larval life.

Methods & Sampling

We cultured Olympia oyster larvae in 50 unique combinations of temperature, salinity, and pCO2. Larval methods are described in growth experiment dataset, but to collect water samples, we measured water temperature and salinity with an Orion Star A329 conductivity meter every two days prior to water changes. We collected pH samples in 20ml plastic scintillation vials, poisoned them 10 µl of mercuric chloride, and stored at room temperature. Roughly one month later, we measured samples for pH using a diode array spectrophotometer (Agilent 8453A UV-VUS) and DIC with a DIC analyzer (Appolo SciTech AS-C3). We then used all parameters collected to calculate pCO2 and Aragonite Saturation with CO2SYS.

Data Processing Description

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- re-formatted date from mdyy to yyyy-mm-dd
- reduced number of significant digits of pH, pCO2, and Ar to reflect sampling precision methods

Data Files

File
GrowthChemistry.csv (Comma Separated Values (.csv), 13.14 KB) MD5:52147ec11739d99f215c1ded3eedca76 Primary data file for dataset ID 776260

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1538626

Deployments

None available yet

Instruments

Apollo SciTech AS-C3 Dissolved Inorganic Carbon (DIC) analyzer

Supplied Name: Appolo SciTech AS-C3 DIC analyzer

Supplied Description:

Instrument Type

Generic Name: Apollo SciTech AS-C3 Dissolved Inorganic Carbon (DIC) analyzer

Acronym: Apollo SciTech AS-C3

Generic Description:

A Dissolved Inorganic Carbon (DIC) analyzer, for use in aquatic carbon dioxide parameter analysis of coastal waters, sediment pore-waters, and time-series incubation samples. The analyzer consists of a solid state infrared CO2 detector, a mass-flow controller, and a digital pump for transferring accurate amounts of reagent and sample. The analyzer uses an electronic cooling system to keep the reactor temperature below 3 degrees Celsius, and a Nafion dry tube to reduce the water vapour and keep the analyzer drift-free and maintenance-free for longer. The analyzer can handle sample volumes from 0.1 - 1.5 milliliters, however the best results are obtained from sample volumes between 0.5 - 1 milliliters. It takes approximately 3 minutes per analysis, and measurement precision is plus or minus 2 micromoles per kilogram or higher for surface seawater. It is designed for both land based and shipboard laboratory use.

Conductivity Meter

Supplied Name: Orion Star A329

Supplied Description:

Instrument Type

Generic Name: Conductivity Meter

Generic Description:

Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.

Spectrophotometer

Supplied Name: Agilent 8453A UV-VUS diode array spectrophotometer

Supplied Description:

Instrument Type

Generic Name: Spectrophotometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Generic Description:

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Cup	Treatment label. Each cup represents a unique temperature/salinity/pCO2 combination	unitless	treatment
Date	Sample date from May 4 - May 21 2018; formatted as yyyy-mm-dd	unitless	date
Cup_Date	Concatenated cup label and date; formatted as Label_mmddyy	unitless	replicate
Temp	Temperature of culture cup water	degrees Celsius	temp
Sal	Salinity measurement of culture cup water	Practical Salinity Units ( PSU)	sal
pH	pH of culture cup water; collected and measured after experiment completion	unitless	pH
pCO2	pCO2 of culture cup water; calculated with pH and DIC in CO2SYS	parts per million (ppm)	pCO2
Ar	Aragonite saturation state; calculated in CO2SYS	unitless	OM_ar
Color	Color treatment of cup tubing. Colors represent treatment levels of high-CO2 air bubbled into treatment cups: yellow=400; blue=800; green=1200; red=1600 ppm CO2 air bubbled into each cup [note: ppm of water doesn’t always equilibrate fully depending on other water parameters].	unitless	treatment

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Database

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Dataset: OlyGrowth_Expt_2018_Chemistry