Dataset: Ichthyoplankton Density - Neuston
Deployment: PE18-21

The NOAA SEAMAP Neuston Plankton Net was deployed according to the "NOAA SEAMAP Field Operations Manual for collection of data, National Marine Fisheries Service and Gulf States Marine Fisheries Commission, March 15, 2016."

The net is deployed so that it is half submerged in the water. Target vessel speed for the net tow is between 1.5-2.0 kts. Target tow time is 10 minutes. Duration of a neuston tow may be shortened to no less than five minutes total tow time if high concentrations of jellyfish, ctenophores, Sargassum, or debris enter the net. The net was deployed over the side.

Nets were rinsed and processed on board. A subset of larval fish was picked on board and frozen at ultra-low temperatures in liquid nitrogen and kept in an on-board Ultra Low Temperature Freezer, transported using a Dry Shipper to Texas A&M University Corpus Christi and stored in an ULT until further processing. The remainder of the sample was preserved in 200 proof Ethanol (ETOH) according to NOAA SEAMAP Operations Manual upon retrieval of nets. After 24 hours, ETOH was exchanged with fresh ETOH. ETOH Plankton samples were stored in 1000ml plastic jars until processing in the Geist Early Life History Research Laboratory at Texas A&M University Corpus Christi during 2017 and 2020. Total Zooplankton Displacement Volume was determined using a calibrated 1000ml measuring cylinder by subtracting the volume of ethanol without sample from the volume of ethanol with sample. The plankton sample was separated from ethanol using a concentrator sieve with a mesh size < than the net mesh size. Then the plankton sample was resuspended in ETOH and larval fish were picked under dissecting microscopes using feather steel tweezers. Every sample was looked through twice for quality check purposes.