Dataset: Nutrients

Name: Nutrient properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana
Published: 2021-02-10
Keywords: nutrients, Barataria Bay, coastal wetlands, wetland submergence, sea level rise, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.840293.1

Spatial Extent: N:29.443547 E:-89.8998 S:29.443547 W:-89.8998

Temporal Extent: 2018-06 - 2018-06

Project:

Fate of Coastal Wetland Carbon Under Increasing Sea Level Rise: Using the Subsiding Louisiana Coast as a Proxy for Future World-Wide Sea Level Projections (Submerged Wetland Carbon)

Principal Investigator:

Lisa G. Chambers (University of Central Florida, UCF)

Co-Principal Investigator:

Robert L. Cook (Louisiana State University, LSU)

John R. White (Louisiana State University Dept. of Oceanography and Coastal Science, LSU-DOCS)

Zuo Xue (Louisiana State University Dept. of Oceanography and Coastal Science, LSU-DOCS)

Student:

Havalend E. Steinmuller (University of Central Florida, UCF)

Contact:

Lisa G. Chambers (University of Central Florida, UCF)

BCO-DMO Data Manager:

Dana Stuart Gerlach (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Taylor Heyl (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2021-02-10

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Nutrient properties of coastal wetland soil cores collected in June 2018 from Barataria Bay, Louisiana

Abstract:

Nine coastal wetland soil cores (150cm) that were collected in June 2018 from Barataria Bay, Louisiana were analyzed for nutrients

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2021-02-10	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.840293.1	2021-05-21

Methods & Sampling

Nine coastal wetland soil cores were collected in June 2018 from Barataria Bay, Louisiana, a shallow open water basin located west of the Mississippi River Delta. Soil cores were collected along three transects, roughly 1 meter apart, that consisted of three points: the coastal fringe (0 m inland), 1 meter inland, and 2 meters inland. Soil cores were collected in polycarbonate tubes via the push core method to a depth of 150 cm, and field-extruded into 15 separate 10-cm intervals. Soils were stored in polyethylene bags on ice and immediately transported back to the laboratory, where they were kept at 4 °C until sample analysis was complete.

This dataset includes analyses of nutrients.

Samples of 2.5 grams of wet soil (both from the field and from the bottle incubation) were placed into 40 mL centrifuge tubes to which 25 mL of 2 M KCl was added. Samples were then shaken continuously on an orbital shaker for 1 hour at 25 °C and 150 rpm, then centrifuged for 10 minutes at 10 °C and 5000 rpm. Following the centrifuge step, samples were immediately filtered through Supor 0.45 μM filters and acidified with double distilled H2SO4 to a pH of < 2 for preservation. Extractable nutrients (nitrate, soluble reactive phosphorus, and ammonium) were then analyzed with an AQ2 Automated Discrete Analyzer (Seal Analytical, Mequon, WI) and using EPA methods 231-A Rev.0, 210-A Rev.1, and 204-A Rev.0.

Data Processing Description

Data processing:
All statistical analysis was performed in R (R Institute for Statistical Computing, Vienna, Austria) using RStudio (RStudio Inc., Boston, MA, USA). The Shapiro-Wilk test was used to verify assumptions of normality, and a logarithmic transformation was performed on all datasets. Levene’s test was used to determine homogeneity of variance. A linear mixed-effect model (package ‘lmer’) was used to test the following predictor variables: depth, distance inland, and the interaction of depth and distance inland on the samples collected from the marsh. Transect was included as a random effect. Distance inland was found to be a non-significant predictor variable for all parameters except for extractable NH4+. As such, distance inland and the interaction of depth and distance was removed as a predictor variable from models except extractable NH4+. Isotopic determinations and quantitative PCR analysis was performed exclusively on the three replicate cores taken 1 m inland, and thus depth was the only predictor variable tested for those parameters. Following determination of significance within one of the predictor variables, the package ‘lsmeans’ was used for post-hoc pairwise comparisons using the Tukey method.

BCO-DMO processing:
- Added a conventional header with dataset name, PI names, version date
- Adjusted parameter names to comply with database requirements.
- Units removed and added to Parameter Description metadata section.
- Added Latitude and Longitude columns, converted DMS to DD
- Added column for Date of sample collection
- Missing data entries replaced with 'nd'

Data Files

File
nutrients.csv (Comma Separated Values (.csv), 9.06 KB) MD5:e38a8f4567bb33dc65070006024a6aed Primary data file for dataset ID 840293

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1635837

Deployments

None available yet

Instruments

Centrifuge

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Centrifuge

Generic Description:

A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids.

Discrete Analyzer

Supplied Name: AQ2 Automated Discrete Analyzer

Supplied Description:

Instrument Type

Generic Name: Discrete Analyzer

Generic Description:

Discrete analyzers utilize discrete reaction wells to mix and develop the colorimetric reaction, allowing for a wide variety of assays to be performed from one sample. These instruments are ideal for drinking water, wastewater, soil testing, environmental and university or research applications where multiple assays and high throughput are required.

Push Corer

Supplied Name:

Supplied Description:

Instrument Type

Generic Name: Push Corer

Generic Description:

Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.

Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/

Shaker

Supplied Name: Orbital Shaker

Supplied Description:

Instrument Type

Generic Name: Shaker

Generic Description:

A Shaker is a piece of lab equipment used to mix, blend, or to agitate substances in tube(s) or flask(s) by shaking them, which is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board which is used to place the flasks, beakers, test tubes, etc.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Location	Site location (29°26_36.77__ N, 89°53_59.28__ W)	unitless	site_descrip
Distance	Distance from marsh edge	meters (m)	no_bcodmo_term
Replicate	Denotation of whether core was the 1st (A), 2nd (B), or 3rd (C) core pulled from the site as field replicate.	unitless	replicate
Depth	Soil depth below the surface.	centimeters (cm)	depth
Extractable_Nitrate	Concentration of extractable nitrate within a soil sample.	miligrams per kilometer (mg kg-1)	NO3
Extractable_SRP	Concentration of extractable soluble reactive phosphorous within a soil sample.	miligrams per kilometer (mg kg-1)	SRP
Extractable_Ammonium	Concentration of extractable ammonium within a soil sample.	miligrams per kilometer (mg kg-1)	NH4
Latitude	Latitude	decimal degrees	lat
Longitude	Longitude (west is negative)	decimal degress	lon
Date_collected	Date of sample collection Format: yyyy-mm-dd	unitless	date

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Dataset: Nutrients