Dataset: Larval Sebastes Otolith and Morphometric Data
Deployment: BBB2104

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/940324

Principal Investigator:

Brice X. Semmens (University of California-San Diego, UCSD-SIO)

Co-Principal Investigator:

Michael R. Landry (University of California-San Diego, UCSD-SIO)

Scientist:

Rasmus Swalethorp (University of California-San Diego, UCSD-SIO)

BCO-DMO Data Manager:

Sawyer Newman (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

RAPID: Understanding the unprecedented anchovy response to warm-water conditions in the California Current (RAPID Anchovy Response)

Version:

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Description

The temporal (date) bounds of this dataset reflect the hatch or capture dates for the specimens represented in the primary data file.

Methods & Sampling

Dataset acquisition description

Specimen Collection

Larval rockfish were collected using a 71cm diameter dual Bongo plankton net system with 505 µm mesh nets and equipped with a small CTD to record depth and physical variables during horizontal net tows at a ship speed of 1.5-2.5 knots down to 28.9-36 m depth.

Collection Location Details

This study is carried out within the temperate to subtropical Southern California Bight and surrounding coastal areas between 32° and 35° N, and 117° and 120° W. Specific locations sampled fall within the CalCOFI and CCE-LTER programs quarterly survey grid from lines 76.7 to 93.4 and stations 26 to 55.

Specimen Processing and Measurement Methods

Once the net was retrieved the nets were gently rinsed with saltwater. The content of the cod-ends were concentrated onto a 300 µm mesh sized sieve, transferred into glass jars and preserved in tris-buffered 95% ethanol. Rockfish larvae were sorted from the 505 µm samples. Sagittal otoliths were extracted from 80 larvae selected to represent a range of sample stations and growth stages. Otoliths were mounted with varnish on labeled microscope slide, photographed using a microscope with an oil immersion lens at 100x magnification and rendered via HeliconFocus v8.1.2. The otolith with the clearest resolution from each larva was selected.

Deployment Details

Each cruise is composed of one or more day trips using one of the two research vessels. The two-three letters indicate the research vessel, the four digits indicates YYMM.

Winter 2021 sampling took place during cruises BBB2101 and BBB2102
Spring 2021 sampling took place during cruses BBB2104 and SW2104

Data Processing Description

Dataset Processing Description

Otolith microstructure was measured using the RFishBC package in R. Age (days) and increment widths were measured by two readers. Otoliths were reexamined in cases where measurements varied substantially between readers. Otolith information from larvae 3 days or younger and/or larvae with empty gut contents were excluded.

Otoliths were mounted with varnish on labeled microscope slide, photographed using a microscope with an oil immersion lens at 100x magnification and rendered via HeliconFocus v8.1.2. The otolith with the clearest resolution from each larva was selected.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-2053719	NSF Division of Ocean Sciences

Instruments

Bongo Net

Supplied Name: Bongo Plankton Net System

Supplied Description:

Larval fish were collected using a 71-centimeter (cm diameter dual Bongo plankton net system equipped with a small CastAway-CTD. The Bongo was equipped with two 505-micrometer (µm) mesh-size nets and a flowmeter mounted in the center of one of them.

Instrument Type

Generic Name: Bongo Net

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/NETT0009/

Generic Description:

A Bongo Net consists of paired plankton nets, typically with a 60 cm diameter mouth opening and varying mesh sizes, 10 to 1000 micron. The Bongo Frame was designed by the National Marine Fisheries Service for use in the MARMAP program. It consists of two cylindrical collars connected with a yoke so that replicate samples are collected at the same time. Variations in models are designed for either vertical hauls (OI-2500 = NMFS Pairovet-Style, MARMAP Bongo, CalVET) or both oblique and vertical hauls (Aquatic Research). The OI-1200 has an opening and closing mechanism that allows discrete "known-depth" sampling. This model is large enough to filter water at the rate of 47.5 m3/minute when towing at a speed of two knots. More information: Ocean Instruments, Aquatic Research, Sea-Gear

Microscope - Optical

Supplied Name: Zeiss Stemi 2000-C Dissecting Microscope

Supplied Description:

Larvae were measured, dissected, and prey identified and measured under a Zeiss Stemi 2000-C dissecting microscope with x50 magnification equipped with a digital camera.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Microscope - Optical

Supplied Name: Microscope with x100 magnification equipped with a digital camera

Supplied Description:

Otoliths were mounted with varnish on labeled microscope slide, photographed using a microscope with an oil immersion lens at 100x magnification and rendered via HeliconFocus v8.1.2.

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

SonTek CastAway-CTD

Supplied Name: CastAway-CTD

Supplied Description:

Larval fish were collected using a dual Bongo plankton net system equipped with a small CTD to record depth and physical variables during horizontal net tows at a ship speed of 1.5 to 2.5 knots down to 14 to 36 meters (m) depth, depending on bottom depth.

Instrument Type

Generic Name: SonTek CastAway-CTD

Acronym: CastAway-CTD

Generic Description:

The Sontek CastAway-CTD (manufactured by Xylem) is a handheld castable instrument that provides instantaneous profiles of temperature, salinity, and sound speed. Each cast is referenced with both time and location using its built-in GPS receiver. The CastAway software displays profiles of the casts in addition to mapping the locations of the data collection points. The CastAway-CTD has a 5 Hz response and sampling rate, accurate to 0.1 (PSS-78), 0.05° Celsius.

Conductivity range is 0 to 100,000 µS/cm.
Temperature range is -5° to 45° Celsius.
Pressure range is 0 to 100 decibars.

Further specs and information can be found on the manufacturer's website: https://www.xylem.com/en-us/brands/wtw/wtw-products/castaway-ctd/

Parameters

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Database

Contribute Data

Dataset: Larval Sebastes Otolith and Morphometric Data
Deployment: BBB2104

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Larval Sebastes Otolith and Morphometric DataDeployment: BBB2104

Dataset acquisition description

Dataset Processing Description

Dataset: Larval Sebastes Otolith and Morphometric Data
Deployment: BBB2104