Dataset: Symbiont density
Deployment: Coffroth_2010

pre- and post-bleach symbiont density in Porites divaricata

Principal Investigator:

Mary Alice Coffroth (State University of New York at Buffalo, SUNY Buffalo)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)

Current State:

Final no updates expected

Version:

2015-01-15

Version Date:

2015-01-15

Data URL:

https://www.bco-dmo.org/dataset-deployment/546136/data

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Description

Cell counts of symbionts by treatment of pre-bleached, bleached and recovering corals (Porites divaricata).

Related Reference:

Coffroth MA, Poland DM, Petrou EL, Brazeau DA, Holmberg JC (2010) Environmental Symbiont Acquisition May Not Be the Solution to Warming Seas for Reef-Building Corals. PLoS ONE 5(10): e13258. doi:10.1371/journal.pone.0013258. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013258

Methods & Sampling

Dataset acquisition description

Collections were made in the vicinity of Long Key, FL, oceanside (N24o 49.791’ W80o 45.743’) and experimentation at the Keys Marine Laboratory, Long Key, FL.

Symbiont density within coral tissues was determined three times over the course of the experiment; (1) before exposure to elevated temperature, (2) when the heat treatment was terminated and (3) 5 weeks into the recovery period. Coral tissue was scraped from the colony surface and placed in 1.0 ml of 5% formalin. The length and width of the scar were measured and these dimensions (length x width) were used to estimate the surface of tissue removed. Subsequently, each tissue sample was homogenized and 9 µL aliquots were counted using a hemacytometer. A total of four replicate counts were conducted per tissue sample and mean symbiont density per mm^2 was calculated.

Data Processing Description

Dataset Processing Description

Cell counts between times (within a treatment) were compared using (repeated measures ANOVA using Greenhouse-Geisser correction for violation of assumption of sphericity, F(1.088,88.144)=137.119, p<0.001; within-subject contrasts, F(1,81)=153.654, p<0.001).

BCO-DMO Processing:

- original file: Coffroth et al 2010_ Data Summary_cell counts.xlsx
- added conventional header with dataset name, PI name, version date, reference information
- changed parameter names to be BCO-DMO compatible
- replaced spaces with underscores - replaced Pur pflex -> Plexaura_flexuosa; BLcntrl -> bleached_control; UBLcntrl -> unbleached control
- combined data from all 3 dates and reformatted to flat file.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-0424996	NSF Division of Ocean Sciences
OCE-0926822	NSF Division of Ocean Sciences

Instruments

Hemocytometer

Supplied Description:

Instrument Type

Generic Name: Hemocytometer

Generic Description:

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
lab	laboratory identification; for mapping puposes	unitless	laboratory
lat	latitude of lab; north is positive	decimal degrees	lat
lon	londitude of lab; east is positive	decimal degrees	lon
description	timing of sample collection: pre- or post-bleaching	unitless	no_bcodmo_term
date	date of sample collection	yyyy-mm-dd	date
sample	sample identification	unitless	sample
culture_inoculant	Cultures 311, 702, A001 and Plexaura flexuosa (Pur Pflex) were obtained from the BURR Culture Collection and used to inoculate bleached colonies of Porites divaricata (Pd).	unitless	treatment
symbiont_type	The symbiont type of the culture used to inoculate bleached colonies of Porites divaricata. Symbiont type based on sequence variation in the chloroplast 23S rDNA (cp-type). Notations based on symbiont clade (Letter) and fragment size (number in bp). For example, A188 is a symbiont within clade A where the 23S rDNA fragment is 188bp in length.	unitless	treatment
count_1	symbiont cells in first subsample	cells	count
count_2	symbiont cells in second subsample	cells	count
count3	symbiont cells in third subsample	cells	count
count_4	symbiont cells in fourth subsample	cells	count
mean_count	average number of cells in sub samples	cells	count
mean_x10000	average number of cells in sub samples x 10^4	cells x 10^4	count
length	length of scar from tissue removal	millimeters	length
width	width of scar from tissue removal	millimeters	width
type	symbiont type: H=?; S=?; LIGHT=?; scar_toss=discarded??	unitless	no_bcodmo_term
surf_area	surface area of coral tissue removed	mm^2	no_bcodmo_term
cells_mm2	symbiont cell density	cells/mm2	abundance
cells_x10000_mm2	symbiont cell density x 10^4	cells x 10^4/mm2	abundance
std_dev	standard deviation of cell density	cells x 10^4/mm2	std_dev

Database

Contribute Data

Dataset: Symbiont density
Deployment: Coffroth_2010

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Symbiont densityDeployment: Coffroth_2010

Dataset acquisition description

Dataset Processing Description

Dataset: Symbiont density
Deployment: Coffroth_2010