Dataset: Grazing experiment 5: pCO2
Deployment: Lab_Olson_B

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.  Some of the culture removed was used to evaluate chemical parameters.  Samples for total alkalinity were taken on growth days 1, 3, 5, 7, 11, 12, 13 and 17 and preserved with HgCl2 and stored at 4° until analysis.  Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel.  DIC samples were filtered through a 0.2 µm nylon syringe filter on the morning of the experimental day, then stored in air tight vials at 4°C until analysis within 60 days using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Other parameters were calculated with CO2sys.