Dataset: Grazing experiment 7: acclimation daily cell counts
Deployment: Lab_Olson_B

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml. Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients. On day 11 Rhodomonas cells from the treatments replicates were pooled then used to inoculate Gyrodinium sp. experiment treatments. Rhodomonas were fed to Gyrodinium at saturating food concentrations (400 µg carbon/liter) and maintained for 5 days in treatment CO2 conditions with daily adjustments of Rhodomonas and media to maintain a steady state Rhodomonas density. Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts. Cells were counted live on a Z2 Coulter Particle Counter. The dilution volume was then calculated to achieve saturating food concentrations.