Dataset: Invertebrate Barcodes from Papua New Guinea

Sampling Devices
This study was conducted in Milne Bay Province, Papua New Guinea at two shallow-water fringing reef localities near Dobu and Upa Upasina (9° 49.45’ S, 150° 49.06’ E) at a depth of 3 meters where almost pure CO2 gas seeps through the seafloor and into the water, changing the water carbonate chemistry and creating sharp gradients in seawater carbonate chemistry parameters within a short distance. Autonomous Reef Monitoring Structures (ARMS) were deployed along the fringing coral reefs at a depth of 4-5 meters at three pH conditions and recovered after approximately 2 years of deployment and all motile organisms greater than 2 millimeters were removed from the structures, photographed, subsampled for genetic analysis, and preserved in 95% ethanol.

Genetic Barcoding
DNA was extracted from tissue subsamples using a standard proteinase-k digestion followed by phenol-chloroform extraction on an AutoGenprep 965. The standard Cytochrome Oxidase Subunit I (COI) barcoding fragment (658 bp) was amplified using the jgLCO1490/jgHCO2198 primer pair. Automated sequencing was performed in both directions directly on purified PCR products using Applied Biosystems BigDye terminator V3.1 Sequence reactions were purified using Millipore 96-well plates loaded with Sephadex G-50 and run on an ABI 3130xl genetic analyzer.