Dataset: Grazing experiment 4: Carbon and Nitrogen
Deployment: Lab_Olson_B

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber filters.  After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks, and capsule blanks) were placed in a drying oven for 24 hours at 60 C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.

This work took place at the Shannon Point Marine Center, Bellingham, Washington.