Dataset: North Pacific Bathycoccus Culture Experiment
Deployment: RR1813

Samples were taken at Ocean Station Papa, Subarctic North Pacific Ocean.  Sampling depth during cruise RR1813 was 3 m. The culture was isolated from a sample taken at approximate location (lat:50, lon:-145). Bathycoccus cells were isolated using a 10 mL pipette after whole seawater sampling from a trace metal clean rosette aboard the R/V Revelle.  Cultures were transported back to the University of North Carolina at Chapel Hill and stored in Aquil media under constant 120-150 µmol photons m-2 d-1 light in a 12ºC incubator.

Cultures were grown with either 1.37x10-6 M total Fe ("Fe+")  or 3.10x10-9 M total Fe ("Fe-") media.  Upon reaching exponential phase growth (based on trends in relative fluorescence units as measured by a Turner fluorometer),  cultures were filtered for chlorophyll a concentration, particulate carbon/particulate nitrogen, and RNA. Additional samples for cell count were collected and fixed with a 10% addition of paraformaldehyde. Measurements of the maximum quantum yield of photochemistry in photosystem II (Fv/Fm) and the functional absorption cross section of photosystem II (sigma) were collected. Filters used in chlorophyll and carbon/nitrogen sampling were uncombusted and combusted Whatman 0.7µm glass fiber filters, respectively.

For RNA sampling, Isopore 0.4 µm 47 mm polycarbonate filters were used. A RNAqueous-4PCR kit and RNeasy MinElute Clean-up kit were used for RNA extraction and clean-up.