Dataset: Invertebrate stable isotopes from Southern California seeps (July 2023)
Deployment: AT50-12

Invertebrate stable isotopes from Southern California seeps (July 2023)

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/945708

Principal Investigator:

Lisa A. Levin (University of California-San Diego Scripps, UCSD-SIO)

Student:

Olívia Soares Pereira (University of California-San Diego Scripps, UCSD-SIO)

BCO-DMO Data Manager:

Audrey Mickle (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems (Methanosphere)

Version:

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Description

Methods & Sampling

Dataset acquisition description

Puschore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator. Rocks were placed into individual compartments in a biobox on the Alvin basket. Before preserving, samples were kept cold and animals were handpicked to sample tissue for stable isotope analyses (see other data files for isotopic data). The tissue (aprox. 0.5-2.5 mg) was placed in pre weighted tin boats or combusted vials (500C overnight) and frozen at -70C. In addition to natural abundance analyses, some animals were placed into mylar bags and exposed to labeled C and N for incubation experiments. These were carried out for 3-8 days, and animals were then processed following the same method as for natural abundance samples. Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters. Filters were frozen for further processing in the laboratory.

Data Processing Description

Dataset Processing Description

In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C. Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis. Microglass fiber filters were oven dried (60C) overnight, and the material on the filters was scrapped off and place int pre-weighed tin boats. Samples were then processed following the same method as for tissue samples.

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-2048720	NSF Division of Ocean Sciences

Instruments

CTD Sea-Bird 911

Supplied Name: CTD/Rosette system

Supplied Description:

Surface and bottom seawater was collected using a CTD/Rosette system and filtered through microglass fiber filters.

Instrument Type

Generic Name: CTD Sea-Bird 911

Acronym: CTD SBE 911

Community Identifier: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0035/

Generic Description:

The Sea-Bird SBE 911 is a type of CTD instrument package. The SBE 911 includes the SBE 9 Underwater Unit and the SBE 11 Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 and SBE 11 is called a SBE 911. The SBE 9 uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 and SBE 4). The SBE 9 CTD can be configured with auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). More information from Sea-Bird Electronics.

Drying Oven

Supplied Name: oven

Supplied Description:

In the laboratory, tissue samples were oven dried (60C) overnight, weighed and acidified with 12.5-25 ul 1N H3PO4 to remove inorganic C.

Instrument Type

Generic Name: Drying Oven

Generic Description:

a heated chamber for drying

HOV Alvin

Supplied Description:

Puschore, rocks, biotubes, and slurp samples were collected with HOV Alvin using the manipulator.

Instrument Type

Generic Name: HOV Alvin

Generic Description:

Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours.

Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed. It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability.

With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor. It can collect data throughout the water column, produce a variety of maps and perform photographic surveys. Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive.

Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor. Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day. Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence

Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology.

(from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09)

Isotope-ratio Mass Spectrometer

Supplied Name: isotope ratio mass spectrometer

Supplied Description:

Stable isotopes measurements (d13C and d15N) were carried out using an isotope ratio mass spectrometer at the Stable Isotope Facility at University of California, Davis.

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

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Database

Contribute Data

Dataset: Invertebrate stable isotopes from Southern California seeps (July 2023)
Deployment: AT50-12

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Invertebrate stable isotopes from Southern California seeps (July 2023)Deployment: AT50-12

Dataset acquisition description

Dataset Processing Description

Dataset: Invertebrate stable isotopes from Southern California seeps (July 2023)
Deployment: AT50-12