Dataset: ETNP 2018-2019 suspended POM concentrations and isotopes
Deployment: RR1805

View Data: For data, See Dataset Metadata Page: https://www.bco-dmo.org/dataset/948637

Principal Investigator:

Clara Fuchsman (University of Maryland Center for Environmental Science, UMCES/HPL)

Project:

Dimensions: Diversity, assembly and function of microbial communities on suspended and sinking particles in a marine Oxygen Deficient Zone (ETNP_ParticleOmics)

Version:

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Description

These data were supported by NSF award DEB-1542240 and Horn Point Laboratory startup funds.

Results publication in review:
Fuchsman, C.A. and Cram, J.A. (in review) Size fractionated suspended organic carbon and nitrogen from the offshore Eastern Tropical North Pacific Oxygen Deficient Zone suggest contributions of picocyanobacteria and vertically migrating metazoans to organic matter. Global Biogeochemical Cycles
*preprint available at ESS Open Archive (Fuchsman & Cram 2024, doi:10.22541/essoar.173046855.50289201/v1)

Methods & Sampling

Dataset acquisition description

Samples were obtained from the Eastern Tropical North Pacific on two cruises. The R/V Revelle cruise RR1805 sampled two stations in April 2018: St P2 (16.9ºN 107ºW) and St P1 (20.3 ºN 106.1ºW). The R/V Kilo Moana cruise KM1920 sampled two stations in October 2019: St P2 (16.9ºN 107ºW) and St P3 (21.8°N 109.9°W).

For depth profiles of bulk suspended particulate organic C and N analyses, 4 L or 10 L (for samples from >300 m) of water was obtained from 12 L Niskin bottles on a CTD rosette, using the spigot of the bottle. Exact volumes were recorded. Water was vacuum filtered onto pre-combusted (400°C), 25 mm diameter, GF/F filters and then frozen. Within a year from collection, samples were wafted with HCl overnight to remove carbonate, dried at 40°C, packed into nested silver and tin capsules, and sent to the UC Davis Stable Isotope Facility (Davis, CA) for C and N analysis utilizing an elemental analyzer (Elementar Vario EL Cube) attached to an isotope ratio mass spectrometer (Isoprim VisION). Blank combusted GF/F filters were included in analyses and did not show measurable material.

Data Processing Description

Dataset Processing Description

The data output from UC Davis was ug C and ug N, as well as the stable isotopes. Microsoft excel was used to convert ug to uM, using the volume of water filtered.

More information about this dataset deployment

Funding

Award Number	Funding Source
DEB-1542240	NSF Division of Environmental Biology

Instruments

Elementar Vario EL Cube elemental analyzer

Supplied Name:

Supplied Description:

At the UC Davis Stable Isotope Facility (Davis, CA), they used an elemental analyzer (Elementar Vario EL Cube) attached to an isotope ratio mass spectrometer (Isoprim VisION).

Instrument Type

Generic Name: Elementar Vario EL Cube elemental analyzer

Acronym: Vario EL Cube

Generic Description:

A laboratory instrument used for quantifying organic elements. It can measure C, H, N and S and optionally O, Cl and TIC. It was first developed in 2006 as a successor to the vario EL III. It uses a high-temperature combustion unit that is able to complete sample digestion at up to 1200 deg C (or 1800 deg C at the point of combustion when tin foil is used) and a jet injection of oxygen directly to the sample during combustion. Separation of gas components are performed on up to 3 gas-selective columns which trap gases until they are heated up and the prior gas peak has reached the baseline during detection. It uses a Thermal Conductivity Detector (TCD) as standard. An infrared (IR) detector for sulfur and oxygen and electrochemical detector for chlorine are optionally available. The instrument can measure C / N elemental ratios of up to 12,000:1 and provides an elemental detection limit of < 40 ppm (TCD).

Isotope-ratio Mass Spectrometer

Supplied Name: Isoprim VisION

Supplied Description:

At the UC Davis Stable Isotope Facility (Davis, CA), they used an elemental analyzer (Elementar Vario EL Cube) attached to an isotope ratio mass spectrometer (Isoprim VisION).

Instrument Type

Generic Name: Isotope-ratio Mass Spectrometer

Acronym: IR Mass Spec; IRMS

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/

Generic Description:

The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise	cruise number	unitless	cruise_id
station	station sampled	unitless	station
Cast	CTD cast	unitless	cast
latitude	location sampled (latitude)	decimal degrees	lat
longitude	location sampled (longitude)	decimal degrees	lon
Date	date sampled (ISO 8601 format)	unitless	date
depth	depth sampled	meters (m)	depth
d13C_VPDB	isotopic composition of C (d13C with respect to reference standard VPDB="Vienna Pee Dee Belemnite")	permil (0/00)	d13C
Carbon	concentration of organic C. Detection limit for C is 30 ug.	micromolar (uM)	C
d15N_Air	isotopic composition of N (d15N with respect to reference standard air).	permil (0/00)	d15N
Nitrogen	concentration of organic N. Detection limit for N is 5 ug	micromolar (uM)	N
C_to_N	ratio of molar carbon to nitrogen concentrations (C:N)	unitless	C_to_N

Database

Contribute Data

Dataset: ETNP 2018-2019 suspended POM concentrations and isotopes
Deployment: RR1805

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: ETNP 2018-2019 suspended POM concentrations and isotopesDeployment: RR1805

Dataset acquisition description

Dataset Processing Description

Dataset: ETNP 2018-2019 suspended POM concentrations and isotopes
Deployment: RR1805