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Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.
The independent variable; one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm). These treatment levels are nominal values as they represent the target pCO2 for each treatment. Within each pCO2 treatment there are 14 days worth of culture measurements for the algal cultures. Measurements of the media (without algae) were made on days 0 and 14. Only chemical measurements were made on the media not biological measurements.