Dataset: O2 consumption rates

To determine potential rates of oxygen consumption, 20 mL of freshly sampled fluids were incubated in sterile, combusted serum vials. For this, fluids were injected into the butyl stoppered serum vials using a 60 mL syringe and hypodermic needle using sterile technique. Overpressure was released by insertion of a second hypodermic needle. Oxygen was monitored optically via optical sensor spots (Presens) mounted to the inner wall of the vials. Fluids not containing particles >3 um diameter and sterile controls were set up as described above but with an additional filter (3 um and 0.2 um pore size respectively) inserted between the syringe outlet and the hypodermic needle. Tubes were incubated in the dark at 5 degrees C.