Dataset: Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.717532.1Version 1 (2022-12-07)Dataset Type:experimental

Principal Investigator: Carol Arnosti (University of North Carolina at Chapel Hill)

BCO-DMO Data Manager: Nancy Copley (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Latitudinal and depth-related contrasts in enzymatic capabilities of pelagic microbial communities: Predictable patterns in the ocean? (Patterns of activities)

Abstract

Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)

Cite Dataset

Fluorescence was measured over 24-48 hours incubation time with a plate reader (TECAN spectrafluor plus; 360 nm excitation, 460 emission), with time points taken every 4-6 hours. Hydrolysis rates were calculated from the rate of increase of fluorescence in the incubation over time relative to a set of standards of known concentration of fluorophore. Scripts to calculate hydrolysis rates and produce the figures shown here are available in the associated Github repository [Hoarfrost, 2017].

See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment: BCO-DMO dataset 717427 (see related datasets).

Related Datasets

References
Dataset: EN556 and EN584 Niskin bottle cast notes
Relationship Description: See Niskin Bottle and Cast List EN584 to link specific casts and bottles to each experiment.
Arnosti, C. (2017) Notes on Niskin bottle sampling and use: depth of sample, operator, observations, type of experiments run on the sample, from RV/Endeavor EN556 and EN584, 2015 and 2016 (Patterns of activities project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-10-20 http://lod.bco-dmo.org/id/dataset/717427

Related Publications

Methods
Hoarfrost, A., Gawarkiewicz, G., & Arnosti, C. (2017, May 15). Ahoarfrost/Shelf1234: Shelf1234 Initial Release. Zenodo. https://doi.org/10.5281/zenodo.580059
Methods
Hoppe, HG. (1993). Use of fluorogenic model substrates for extracellular enzyme activity (EEA) measurement of bacteria, p. 423-431. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, FL 978-0873715645
Methods
Obayashi, Y., & Suzuki, S. (2005). Proteolytic enzymes in coastal surface seawater: Significant activity of endopeptidases and exopeptidases. Limnology and Oceanography, 50(2), 722–726. doi:10.4319/lo.2005.50.2.0722

Arnosti, C. (2022) Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-12-07 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.717532.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation