Dataset: Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.806510.1Version 1 (2020-04-21)Dataset Type:Cruise Results

Principal Investigator: Mak A. Saito (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Amber D. York (Woods Hole Oceanographic Institution)

Program: U.S. GEOTRACES (U.S. GEOTRACES)

Program: Marine Microbiology Initiative (MMI)

Project: Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients (GPc03) (MetZyme)

Project: The ProteOMZ Expedition: Investigating Life Without Oxygen in the Pacific Ocean (ProteOMZ (Proteomics in an Oxygen Minimum Zone))

Project: Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)

Project: US GEOTRACES PMT: Cobalt Biogeochemical Cycling and Connections to Metalloenzymes in the Pacific Ocean (PMT Cobalt and Metalloenzymes)

Project: Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)

Project: Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions (Cyanobacteria Warming Responses)

Project: Collaborative Research: Underexplored Connections between Nitrogen and Trace Metal Cycling in Oxygen Minimum Zones Mediated by Metalloenzyme Inventories (CliOMZ)

Abstract

Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016. NxrA and NxrB peptide concentrations in fmol/L. Peptide names are using the GEOTRACES naming convention (PEP for peptide, full tryptic peptide amino acid sequence, Protein name, Sampling device (=Pump)). Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit. These data were pu...

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Cite Dataset

Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016. NxrA and NxrB peptide concentrations in fmol/L. Peptide names are using the GEOTRACES naming convention (PEP for peptide, full tryptic peptide amino acid sequence, Protein name, Sampling device (=Pump)). Quality flags follow each peptide column and use the GEOTRACES convention of 1 for good, 6 for below detection limit. These data were published in Saito et al., 2020 as Supplementary Table 1.

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Related Publications

Results
Saito, M. A., McIlvin, M. R., Moran, D. M., Santoro, A. E., Dupont, C. L., Rafter, P. A., … Waterbury, J. B. (2020). Abundant nitrite-oxidizing metalloenzymes in the mesopelagic zone of the tropical Pacific Ocean. Nature Geoscience, 13(5), 355–362. doi:10.1038/s41561-020-0565-6
Methods
Gasteiger, E., Hoogland, C., Gattiker, A., Wilkins, M. R., Appel, R. D., & Bairoch, A. (2005). Protein identification and analysis tools on the ExPASy server. In The proteomics protocols handbook (pp. 571-607). Humana press.
Methods
Nomenclature and Symbolism for Amino Acids and Peptides. Recommendations 1983. (1984). European Journal of Biochemistry, 138(1), 9–37. doi:10.1111/j.1432-1033.1984.tb07877.x
Methods
SIB Swiss Institute of Bioinformatics. (n.d.). Compute pI/Mw tool. SIB Swiss Institute of Bioinformatics. Retrieved March 27, 2020, from https://web.expasy.org/compute_pi/
Methods
SIB Swiss Institute of Bioinformatics. (n.d.). ProtParam tool. SIB Swiss Institute of Bioinformatics. Retrieved March 27, 2020, from https://web.expasy.org/protparam/

Saito, M. (2020) Nitrite Oxidoreductase targeted metaproteomics from R/V Kilo Moana cruise KM1128 and R/V Falkor cruise FK160115 in the Central Pacific Ocean in 2011 and 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2020-04-21 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.806510.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


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Funded by the U.S. National Science Foundation