Dataset: Kelp forest pumping data - DIC

Continuous flow pumping experiments were conducted inside the kelp forest from a vessel within 15-m of the kelp mooring to obtain high temporal and vertical resolution biogeochemical data. Two experiments were conducted (July 11-13 and July 18-20, 2018) that overlapped with the kelp mooring pH timeseries data. Seawater was pumped from five depths spanning the water column using five sections of equal-length polypropylene tubing (3/8” ID, 1/2” OD) deployed over the side of a moored vessel. The depths presented here include the surface (valve #5), 6 mab (2-5 mbs, valve #3), and 1 mab (7-10 mbs, valve #1). A custom auto sampling manifold introduced water from each of the 5 tubes to a continuous flow system at 5-minute intervals, allowing the full suite of depths to be sampled every 25 minutes for the duration of each experiment (similar to Koweek et al., 2015a; Koweek et al., 2015b; Teneva et al., 2013). Seawater was drawn into the continuous flow system from each depth by a peristaltic pump operating at 2L/min.

Dissolved inorganic carbon (DIC) samples were automatically drawn every 5 minutes and analyzed using a custom-built sample acidification and delivery system coupled to a Li-COR 7000 infrared gas analyzer as described in Long et al. (2011). DIC was calibrated every hour using certified reference materials (CRM), Batch 169 (Dickson, 2010). Instrumental precision, based on 102 CRM analyses, was ± 5.7 μmol kg-1.