Dataset: Bottle AR29

Location: New England Shelfbreak 40 S 71W depth : 0-2000m.

Twenty-four 10 L Niskin bottles fitted with Teflon-coated external closures were used for water column sampling. At each station, samples were typically collected at 12 discrete depths for assessment of nutrient concentrations. These samples were syringe-filtered and stored at -20°C  until analysis at the WHOI Nutrient Analytical Facility. Nitrate and silicate were measured using standard AutoAnalyzer techniques. To measure ammonium concentrations, site water was cartridge-filtered (0.1 µm, Pall Co.) directly from Niskin bottles using a peristaltic pump. Filtrate was collected in FalconTM tubes that were pre-treated with orthophthaldialdehyde (OPA) and measured on-board via the OPA method (Holmes et al., 1999) with a detection limit of 10 nM.

To measure particulate organic carbon and nitrogen, water was collected from the Niskin bottles and filtered through combusted 0.7 µm glass fiber filters (Whatman GF/F), rinsed with a weak acid (0.01 N HCl in seawater) to remove carbonates, then dried in combusted glass vials at 60 °C. Diatom biomass was assessed by sampling for biogenic silica. Samples were filtered through 0.6 µm polycarbonate filters, dried at 60°C in plastic Petri dishes, and dissolved in strong acid. 

For incubation-based primary productivity, water samples were taken from Niskin bottles at known isolumes, then placed in sterile 285 mL Qorpak bottles, then ~20 µCi NaH14CO3 was added. An on-deck incubator holding the bottles had surface seawater flowing through it, with irradiance attenuated by neutral density filters to the light levels at the isolumes sampled. Blue filters were used for isolumes below 30% Eo. After 24 h, samples were filtered through GFF filters and placed in 7 mL scintillation vials. Size fractionations were conducted at all stations using 20 µm Poretics filters on subsamples from each bottle. 100 µL 1N HCl was added to volatilize absorbed inorganic 14C. Ecolume (5 mL) was then added to each vial, and all vials were counted after 24 h on a liquid scintillation counter. Total activity was measured by counting 100 μL of non-acidified sample in β-phenethanylamine.