Dataset: Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the specific metabolites riboflavin, pantothenic acid, and caffeine.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.865159.1Version 1 (2022-11-10)Dataset Type:experimental

Principal Investigator: Amy Apprill (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Elizabeth Kujawinski (Woods Hole Oceanographic Institution)

Scientist, Contact: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Signature exometabolomes of Caribbean corals and influences on reef picoplankton (Coral Exometabolomes)

Abstract

Pre-filtered reef seawater microbial communities collected from Lameshur Bay, U.S. Virgin Islands were incubated separately in the presence of the individual metabolites riboflavin, pantothenic acid, and caffeine for 24 hours and samples were collected to monitor changes in microbial community composition using 16S rRNA gene sequencing and microbial abundances using flow cytometry. Targeted metabolomic data from these incubations is available on the MetaboLights database under accession number ...

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Note: This flow cytometry data is the raw data

Other relevant files and publications:

The targeted and untargeted metabolomics data and metadata associated with this study are located on the MetaboLights database under accession numbers MTBLS2855 and MTBLS3286.

The 16S rRNA gene sequencing data and metadata associated with this study are located on the NCBI Sequence Read Archive (SRA) under BioProject PRJNA739882. BioSample accession numbers are not linked with the data submitted to BCO-DMO because samples for flow cytometry and macronutrients were not always collected at the same time as samples collected for microbial community analyses, meaning that only some of the samples collected for microbial community analyses have affiliated microbial abundances and nutrient concentrations.

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Related Publications

Results
Weber, L., Soule, M. K., Longnecker, K., Becker, C. C., Huntley, N., Kujawinski, E. B., & Apprill, A. (2022). Benthic exometabolites and their ecological significance on threatened Caribbean coral reefs. ISME Communications, 2(1). https://doi.org/10.1038/s43705-022-00184-7
Methods
Apprill, A., & Rappé, M. (2011). Response of the microbial community to coral spawning in lagoon and reef flat environments of Hawaii, USA. Aquatic Microbial Ecology, 62(3), 251–266. doi:10.3354/ame01471
Methods
Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015). Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquatic Microbial Ecology, 75(2), 129–137. doi:10.3354/ame01753
Methods
Campbell, L., & Vaulot, D. (1993). Photosynthetic picoplankton community structure in the subtropical North Pacific Ocean near Hawaii (station ALOHA). Deep Sea Research Part I: Oceanographic Research Papers, 40(10), 2043–2060. doi:10.1016/0967-0637(93)90044-4
Methods
Campbell, L., Nolla, H. A., & Vaulot, D. (1994). The importance of Prochlorococcus to community structure in the central North Pacific Ocean. Limnology and Oceanography, 39(4), 954–961. doi:10.4319/lo.1994.39.4.0954

Weber, L., Apprill, A., Kujawinski, E. (2022) Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the specific metabolites riboflavin, pantothenic acid, and caffeine.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-11-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865159.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


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