Dataset: Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the mixed exudates released from the coral species Porites astreoides and Gorgonia ventalina.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.865739.1Version 1 (2022-11-10)Dataset Type:experimental

Principal Investigator: Amy Apprill (Woods Hole Oceanographic Institution)

Co-Principal Investigator: Elizabeth Kujawinski (Woods Hole Oceanographic Institution)

Scientist, Contact: Laura Gray (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)

Project: Signature exometabolomes of Caribbean corals and influences on reef picoplankton (Coral Exometabolomes)

Abstract

Incubation experiments were conducted in St. John, US Virgin Islands to investigate the composition of exudates released from different species of benthic organisms, and the response of reef seawater microbial communities to mixed exudates released from different species and to specific metabolites. Exudates were collected from the stony coral Porites astreoides, and the octocoral Gorgonia ventalina after an 8 hour incubation. Reef seawater microbial communities were incubated separately in the ...

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Cite Dataset

The flow cytometry data in this dataset is the raw data and has not been normalized (as is presented in the manuscript Weber et al., 2022).

Other relevant files and publications:

The targeted and untargeted metabolomics data and metadata associated with this study are located on the MetaboLights database under accession numbers MTBLS2855 and MTBLS3286.

The 16S rRNA gene sequencing data and metadata associated with this study are located on the NCBI Sequence Read Archive (SRA) under BioProject PRJNA739882. BioSample accession numbers are not linked with the data submitted to BCO-DMO because samples for flow cytometry and macronutrients were not always collected at the same time as samples collected for microbial community analyses, meaning that only some of the samples collected for microbial community analyses have affiliated microbial abundances and nutrient concentrations.

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Related Publications

Results
Weber, L., Soule, M. K., Longnecker, K., Becker, C. C., Huntley, N., Kujawinski, E. B., & Apprill, A. (2022). Benthic exometabolites and their ecological significance on threatened Caribbean coral reefs. ISME Communications, 2(1). https://doi.org/10.1038/s43705-022-00184-7
Methods
Apprill, A., & Rappé, M. (2011). Response of the microbial community to coral spawning in lagoon and reef flat environments of Hawaii, USA. Aquatic Microbial Ecology, 62(3), 251–266. doi:10.3354/ame01471
Methods
Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015). Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquatic Microbial Ecology, 75(2), 129–137. doi:10.3354/ame01753
Methods
Campbell, L., & Vaulot, D. (1993). Photosynthetic picoplankton community structure in the subtropical North Pacific Ocean near Hawaii (station ALOHA). Deep Sea Research Part I: Oceanographic Research Papers, 40(10), 2043–2060. doi:10.1016/0967-0637(93)90044-4
Methods
Campbell, L., Nolla, H. A., & Vaulot, D. (1994). The importance of Prochlorococcus to community structure in the central North Pacific Ocean. Limnology and Oceanography, 39(4), 954–961. doi:10.4319/lo.1994.39.4.0954

Weber, L., Apprill, A., Kujawinski, E. (2022) Incubation experiments were conducted in St. John, US Virgin Islands to investigate the response of reef seawater microbial communities to the mixed exudates released from the coral species Porites astreoides and Gorgonia ventalina.. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-11-10 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.865739.1 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation