Dataset: Gross oxygen production

Name: Gross oxygen production and total daytime oxygen consumption derived from in vitro incubations deployed in situ at different depths at Station ALOHA during June 2019 (cruise ID KM1910)
Published: 2022-05-25
Keywords: primary production, gross oxygen production, respiration, station ALOHA, North Pacific Subtropical Gyre, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.868714.1

Spatial Extent: N:22.81 E:-157.92 S:22.72 W:-157.95

Temporal Extent: 2019-06-19 - 2019-06-22

Project:

EAGER Collaborative Research: Early career chief scientist training for biological and chemical oceanographers (Chief Sci KM1910)

Principal Investigator:

Sara Ferrón (University of Hawaii at Manoa, SOEST)

Contact:

Sara Ferrón (University of Hawaii at Manoa, SOEST)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2022-05-25

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Gross oxygen production and total daytime oxygen consumption derived from in vitro incubations deployed in situ at different depths at Station ALOHA during June 2019 (cruise ID KM1910)

Abstract:

Gross oxygen production and total daytime oxygen consumption derived from in vitro incubations deployed in situ at different depths at Station ALOHA during June 2019 (cruise ID KM1910)

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2022-05-25	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.868714.1	2022-05-25

Description

Rates of gross oxygen production rates and total daytime oxygen consumption were measured using the 18O-water in vitro method and membrane inlet mass spectrometry as described by Ferrón et al. (2016).

Methods & Sampling

Seawater samples for the incubations were collected in triplicate before dawn at six different depths (5, 25, 45, 75, 100, and 125m) with 12-L Niskin® bottles attached to a CTD rosette. Subsamples were taken from the Niskin bottles in acid-washed volume- calibrated 150-mLquartz glass bottles with ground-glass stoppers. The bottles were first rinsed with the seawater sample, and then filled from bottom to top using acid- washed silicon tubing, allowing the water to overflow at least twice the volume of the bottles. Before closing, the incubation bottles were spiked with 650 µL of ¹⁸O-H₂O (97.2% ¹⁸O, Medical Isotopes) for the surface samples (5-45 m) and 1,000 µL for deeper samples (75-125 m). The samples were deployed in situ at the corresponding depths before dawn using a free-drifting array, and incubated until dawn. After recovery, the incubated samples were poisoned with saturated mercuric chloride solution to inhibit biological activity. Time-zero samples were also collected pre-dawn and poisoned at the time of the deployment.

Data Processing Description

Data is processed as described by Ferrón et al. (2016). Gross oxygen production is determined from the change in the 18O isotope ratio of dissolved oxygen over the incubation:

GOP = [R(O2)final - R(O2)initial / R(H2O) - R(O2)initial ] x [O2]initial

where R(O2)initial and R(O2)final are the initial and final isotope ratios (18O/16O) for dissolved oxygen, respectively, [O2]initial is the initial dissolved oxygen concentration, and R(H2O) is the 18O isotope ratio of the incubation water.

The net oxygen change during the incubation is simultaneously determined from the net change in oxygen to argon molar ratios:

NOC = [ ((O2/Ar)final / (O2/Ar)initial) - 1 ] x [O2]initial

where (O2/Ar)final and (O2/Ar)initial are the final and initial oxygen to argon molar ratios, respectively.

The total daytime oxygen consumption is derived as the difference between GOP and NOC and extrapolated to 24 hours (Ferrón et al., 2016).

BCO-DMO Processing Notes:
* Adjusted column names to comply with database requirements
* Added ISO8601 format of date and times

Data Files

File
oxygen_production.csv (Comma Separated Values (.csv), 3.22 KB) MD5:b175a5d49dd9ef55bb4cbf035960bb70 Primary data file for dataset ID 868714

Supplemental Files

File
Gross_oxygen_production_km1910 filename: Gross_oxygen_production_km1910.xlsx (Octet Stream, 12.57 KB) MD5:3ff67ba40dbc44a066292f0d5c00aa27 Gross oxygen production km1910: Data table with mean and standard deviation of three replicates of parameters GOP and R.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1911990

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
KM1910	EAGER Chief Sci cruise 2019 Chief Scientist Training Cruise	2019-06-15	R/V Kilo Moana	Matthew J. Church (Chief Scientist) Erica Goetze (Co-Chief Scientist) Angelicque E. White (Co-Chief Scientist)	data info

Instruments

Membrane Inlet Mass Spectrometer

Supplied Name: Membrane inlet mass spectrometer

Supplied Description:

Membrane inlet mass spectrometer consists of a Pfeiffer Vacuum HiCube 80 Eco turbo pumping station connected to a HiQuayTM quadrupole mass spectrometer (QMG700), with a Balzers radio frequency generator (QMH 400-5) and a Balzers analyzer (QMA 430). The membrane inlet design is from Bay Instruments (Easton, Maryland).

Instrument Type

Generic Name: Membrane Inlet Mass Spectrometer

Acronym: MIMS

Generic Description:

Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Cruise_ID	Cruise identification number	unitless	cruise_id
Date	Date of the incubations Format: %m-%d-%y	UTC	date
Latitude	Array deployment latitude, south is negative	decimal degrees	lat
Longitude	Array deployment Longitude, west is negative	decimal degrees	lon
Time_in	Local time of array deployment in HST (UTC-10h) Format: %H:%M	unitless	time
Time_out	Local time of array recovery in HST (UTC-10h) Format: %H:%M	unitless	time
Depth	Sampling and incubation depth	meters	depth
GOP	Gross oxygen production	mmol O2 m-3 d-1	O2
R	Total daytime O2 consumption in the light	mmol O2 m-3 d-1	O2
ISO_DateTime_UTC_In	Sampling start date and time (UTC) in ISO8601 format: YYYY-MM-DDThh:mmz Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC
ISO_DateTime_UTC_Out	Sampling start date and time (UTC) in ISO8601 format: YYYY-MM-DDThh:mmz Format: %Y-%m-%dT%H:%MZ	unitless	ISO_DateTime_UTC

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Database

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Dataset: Gross oxygen production