Dataset: Ocean warming & acidification experiment: Acartia tonsa egg production and hatching success

Name: Acartia tonsa egg production rate and egg hatching success for transgenerational exposure to ocean warming and ocean acidification
Published: 2023-09-07
Keywords: copepod, adaptation, climate change, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.906780.1

Project:

Collaborative Research: Response of marine copepods to warming temperature and ocean acidification (Copepod Response to Warming Temp and OA)

Principal Investigator:

Hans G. Dam (University of Connecticut, UConn)

Co-Principal Investigator:

Hannes Baumann (University of Connecticut, UConn)

Michael Finiguerra (University of Connecticut, UConn)

Melissa Pespeni (University of Vermont, UVM)

Scientist:

Reid Brennan (University of Vermont, UVM)

Student:

James deMayo (University of Connecticut, UConn)

Gihong Park (University of Connecticut, UConn)

Contact:

James deMayo (University of Connecticut, UConn)

Technician:

Lydia Norton (University of Connecticut, UConn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-09-07

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Acartia tonsa egg production rate and egg hatching success for transgenerational exposure to ocean warming and ocean acidification

Abstract:

These data include egg production rate (EPR) and egg hatching success (HS) data for Acartia tonsa during multigenerational exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments. Data was collected as the number of eggs produced and hatched offspring per female per treatment at each generation.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-09-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.906780.1	2023-09-07
2023-09-07	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.906780.1	2023-09-07

Description

These data are part of at multigenerational experiment of Arcatia tonsa exposure to ocean warming (OW), ocean acidification (OA), and combined ocean warming and acidification (OWA) including a benign ambient condition temperature and CO2 control (AM). These data were collected every third generation between F0 and F15 and at F25 for all treatments.

Methods & Sampling

For each replicate culture within a treatment, 10 pairs of newly developed males and females were placed into 20 mL petri dishes for 48 h (n = 280 per treatment). The dishes were housed in custom-made, airtight, plexiglass enclosures whose atmosphere was controlled to the appropriate CO2 concentration. There was one enclosure per temperature-controlled incubator. After the 48-h egg laying period, adults were checked for survival and removed from the petri dishes. Eggs were left in the dishes for an additional 72 h to allow for egg hatching and their contents preserved with non-acid Lugol’s solution. Dishes with dead males were used for EPR, but not HS, since fertilization could not be assumed.

Data Processing Description

Dishes with dead females were discarded. EPR was calculated as (Eu + Eh)/t where Eu represents unhatched eggs, Eh represents hatched eggs (nauplii), and t represents egg laying time. Hatching success was calculated as Eh. All data was processed in R v4.0.2

Data Files

File
906780_v1_dam_eggprod.csv (Comma Separated Values (.csv), 31.51 KB) MD5:73865cfe814b672dd302b59968c573bc Primary datafile for datase 906780

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1559075
NSF Division of Ocean Sciences	OCE-1559180

Deployments

None available yet

Instruments

Microscope - Optical

Supplied Name: Olympus SZH-ILLD stereo microscope

Supplied Description:

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Generation	The corresponding generation when survival was measured	unitless	stage
Rep	The corresponding treatment evaluated	unitless	replicate
Treatment	Target temperature for each treatment	unitless	treatment
Temp	Target pH for each treatment	degrees Celsius (°C)	temp
pH	Biological culture replicate for each treatment	unitless	pH
Hatched	Number of hatched eggs observed	unitless	count
Unhatched	Number of unhatched eggs observed	unitless	count
Total	Sum of "Hatched" and "Unhatched" columns	unitless	count
EPRtot	Rate of eggs produced per female per day	unitless	ratio
HFtot	Frequency of hatching success	unitless	ratio