Dataset: Karlodinium veneficum cellular carbon and nitrogen data

Name: Cellular carbon (C) and nitrogen (N) data of the Karlodinium veneficum growth experiment conducted in the Horn Point Laboratory between June 2021- January 2022.
Published: 2023-09-01
Keywords: harmful algal blooms, climate change, Karlodinium veneficum, oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.907463.1

Temporal Extent: 2021-06-11 - 2021-11-12

Project:

Ecology and Oceanography of Harmful Algal Blooms (EcoHAB)

Principal Investigator:

Patricia A. Glibert (University of Maryland Center for Environmental Science, UMCES/HPL)

Scientist:

Nayani Vidyarathna (University of Maryland Center for Environmental Science, UMCES/HPL)

Student:

So Hyun Ahn (University of Maryland Center for Environmental Science, UMCES/HPL)

Contact:

Nayani Vidyarathna (University of Maryland Center for Environmental Science, UMCES/HPL)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-09-01

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Cellular carbon (C) and nitrogen (N) data of the Karlodinium veneficum growth experiment conducted in the Horn Point Laboratory between June 2021- January 2022.

Abstract:

Using a multifactorial design, we studied the interactive effects of temperature (15, 20, 25, 28 and 30 0C), salinity (5, 10, 15, 20 and 30) and light (low-100 and high-300 μmol photons m−2 s−1) on growth, thermal niche properties and cellular carbon (C) and nitrogen (N) of the toxic dinoflagellate, Karlodinium veneficum, originated from the Chesapeake Bay. Here we report the daily growth data measured as raw fluorescence at each experimental combination in quadruplicates and the C and N cell quota data measured in the final samples for each experimental combination in duplicates.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2023-09-01	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.907463.1	2023-09-01

Description

These data (after processing) were published in Vidyarathna et al (2023) as Figure 1, Figure 3 and supplementary table 1 and 3.

Methods & Sampling

Samples (15 mL) for cellular organic C and N were filtered onto 25 mm pre-combusted glass-fiber filters (2h at 450 °C, GF/F, Whatman), dried at 60 °C for 24 h and then stored in a desiccator prior to analyses. Cellular C and N were then quantified with a CHN elemental analyzer (ECS 4010 Elemental combustion system; Costech Instruments, USA), with phenylalanine and EDTA used as standards.

Data Processing Description

No data processing, these are the raw data.

Data Files

File
907463_v1_chn.csv (Comma Separated Values (.csv), 8.20 KB) MD5:5a468afbfa831313c18a59c85059c0da Primary data file for dataset 907463.

More Information about this dataset

Funding Sources

Funding Source	Award Number
National Oceanic and Atmospheric Administration	NA17NOS4780180

Deployments

None available yet

Instruments

CHN Elemental Analyzer

Supplied Name: CHN elemental analyzer- ECS 4010 Elemental combustion system; Costech Instruments, USA

Supplied Description:

Instrument Type

Generic Name: CHN Elemental Analyzer

Acronym: CHN_EA

Generic Description:

A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
sample_no	Sample number (id)	unitless	sample
treatment	Culture condition (salinity, light and temperature). LL=Low light (100), HL=High light (300)	unitless	treatment
filtered_volume	Volume filtered from each culture replicate	milliliters (mL)	vol_filt
Cell_density	Final cell density of each culture	cells per mL (cells/mL)	density
N_percent	Percentage of N in the sample (filter) analyzed	percent (%)	N
C_percent	Percentage of C in the sample (filter) analyzed	percent (%)	C
N_mg	weight of N in the sample (filter) analyzed	milligrams (mg)	N
C_mg	weight of C in the sample (filter) analyzed	milligrams (mg)	C
Carbon_blank_corrected	blank corrected weight of C in the sample (filter) analyzed	milligrams (mg)	C
C_to_N_mass	C/N ratio (mass)	unitless	C_to_N
C_umol	C content converted to umol	micromoles (umol)	C
N_umol	Ncontent converted to umol	micromoles (umol)	N
C_to_N_atomic	C/N ratio (atomic)	unitless	C_to_N
C_per_cell	C content nnormalized per cell	picograms of Carbon per cell (pg/cell)	C
N_per_cell	Ncontent nnormalized per cell	picograms of Nitrogen per cell (pg/cell)	N