Dataset: Nematostella ocean acidification experiment: Larval respiration

Name: Larval respiration performance of Nematostella vectensis following parental exposure to ocean acidification during lab experiments conducted in spring 2022.
Published: 2024-03-24
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.920260.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-24

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Larval respiration performance of Nematostella vectensis following parental exposure to ocean acidification during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset pertains to the performance of larvae following parental exposure to ocean acidification, specifically larval respiration.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2024-03-24	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.920260.1	2024-03-24

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

To quantify larval respiration rates, sperm and eggs were first combined by treatment in glass finger bowls in weeks 11 and 13. At 3 DPF, swimming larvae from each parental treatment were pipetted in three groups of ten (week 11) or nine groups of 15 (week 13) into wells of a 24-well plate equipped with oxygen sensor spots (Loligo Systems, Viborg, Denmark).

Wells containing larvae as well as larvae-free wells containing water from the fertilization bowl as a bacterial control (‘blanks’; N=3 in week 11; N=6 in week 13) were filled to capacity (80 μL) with 12 ppt ASW and sealed with an adhesive plate cover before being placed on a PreSens SensorDish^Ⓡ Reader (Precision Sensing, Regensburg, Germany), which was previously calibrated according to the manufacturer's instructions.

Dissolved oxygen concentrations in each well were read every 15 s for at least 1 h, during which no wells experienced near or total oxygen depletion. The rate of oxygen consumption over time was determined from the slopes of linear regressions of oxygen levels multiplied by the volume of the wells. The average oxygen consumption rate for the blank wells was subtracted from the larval rates, which were then converted to nmol O₂ minute⁻¹ larva⁻¹.

For respiration data, the average oxygen consumption rate for the blank wells was subtracted from the larval rates, which were then converted to nmol O₂ minute⁻¹ larva⁻¹

BCO-DMO Processing

* Adjusted parameter names to comply with database requirements

Data Files

File
920260_v1_larvalrespiration.csv (Comma Separated Values (.csv), 801 bytes) MD5:a2b4c6931681b5061d35a62e430519c0 Primary data file for dataset ID 920260, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

PreSens OXY-10 Mini oxygen meter

Supplied Name: PreSens SensorDishⓇ Reader

Supplied Description:

PreSens SensorDishⓇ Reader (Precision Sensing, Regensburg, Germany) for respiration measurements

Instrument Type

Generic Name: PreSens OXY-10 Mini oxygen meter

Generic Description:

The OXY-10 mini is a precise multi-channel oxygen meter for up to 10 'in-house' sensors, simultaneously controlling and reading them. The meter is used with oxygen sensors based on a 2mm optical fibre. The meter is compatible with sensors that are type PSt3 which has a detection limit 15 ppb, 0 - 100% oxygen.

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
Date	Sampling week (week 1 start = 2022-01-26)	unitless	time_point
Respiration_Rate_nmol_O2_per_minute	Rate of oxygen consumption of anemones due to respiration, quantified as nmol oxygen consumed per minute	nmol oxygen consumed per minute	resp

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Dataset: Nematostella ocean acidification experiment: Larval respiration