Dataset: Nematostella ocean acidification experiment: Egg Size per Bundle

Name: Eggs size per bundle measurements following parental ocean acidification during lab experiments conducted in spring 2022.
Published: 2024-03-26
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.923267.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-26

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Eggs size per bundle measurements following parental ocean acidification during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset pertains to the reproductive physiology of adult organisms from this study: egg size per bundle.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2024-03-26	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.923267.1	2024-03-26

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

Egg bundles were placed in individual wells of a 24-well plate with a plastic transfer pipette following spawning. Images of each bundle were collected using a Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA) attached to a Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany). The images were then analyzed for egg counts per bundle (size of eggs in the bundle of eggs produced by the same female) manually in Fiji. For egg sizes, a 1×1 mm grid was photographed as a size standard to calibrate images, the line tool was used to draw the diameter of each egg, and the length of the line was recorded. For weeks 2 and 4, 60 eggs were measured across all females, whereas 30 eggs per female were measured for weeks 7–13

Data Files

File
923267_v1_eggbundle.csv (Comma Separated Values (.csv), 3.02 KB) MD5:f3ec9791675280f25f7aaee07752debb Primary data file for dataset ID 923267, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

Camera

Supplied Name: Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA)

Supplied Description:

Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA) attached to a Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany) for images

Instrument Type

Generic Name: Camera

Acronym: camera

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/311/

Generic Description:

All types of photographic equipment including stills, video, film and digital systems.

Microscope - Optical

Supplied Name: Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany

Supplied Description:

Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA) attached to a Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany) for images

Instrument Type

Generic Name: Microscope - Optical

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/

Generic Description:

Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
Date	Sampling week (week 1 start = 2022-01-26)	unitless	time_point
Average_egg_size	Average size of eggs in a single bundle released by a single female	centimeter (cm)	length
Number_of_eggs	Number of eggs in the same bundle for which average size was calculated	number of egss	count

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Database

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Dataset: Nematostella ocean acidification experiment: Egg Size per Bundle

Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA) attached to a Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany) for images

Retiga R3 CCD camera (Meyer Instruments, Houston, TX, USA) attached to a Leica MZ12 dissecting microscope (Leica Camera, Wetzlar, Germany) for images