Dataset: High-throughput sequencing of the 16S rRNA gene, microscopy, and flow cytometry of pyrosome-associated microorganisms compared to seawater sampled during a Pyrosoma atlanticum bloom in the Northern California Current System in July 2018.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.926093.1Version 1 (2024-05-14)Dataset Type:Other Field Results

Co-Principal Investigator, Contact: Anne W. Thompson (Portland State University)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey (Gelatinous Grazer Feeding)


Abstract

Pyrosomes are widely distributed pelagic tunicates that have the potential to reshape marine food webs when they bloom. However, their grazing preferences and interactions with the background microbial community are poorly understood. The diversity, relative abundance, and taxonomy of pyrosome-associated microorganisms were compared to seawater during a Pyrosoma atlanticum bloom in the Northern California Current System using high-throughput sequencing of the 16S rRNA gene, microscopy, and flow ...

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Pyrosomes and seawater were collected from the NCC, off the Oregon Coast, in July 2018, near the peak of a multiyear bloom of P. atlanticum. Samples were collected from the R/V Sally Ride (SR1810) along the Newport Hydrographic Line. Station D5 (Cast 20, 44.652141N; 125.117573W) was sampled on July 9, 2018 in the presence of a strong temperature gradient in the top 20 meters and a chlorophyll peak at 17 meters. Station D3 (Cast 26, 44.651756N; 124.589313W) was sampled on July 11, 2018 in the presence of a mixed layer depth of 15 meters and a chlorophyll peak at the base of the mixed layer.


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Results

Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA659246
Portland State University. Pyrosome microbiome Raw sequence reads. 2020/08. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA659246. NCBI:BioProject: PRJNA659246.

Related Publications

Results

Thompson, A. W., Ward, A. C., Sweeney, C. P., & Sutherland, K. R. (2021). Host-specific symbioses and the microbial prey of a pelagic tunicate (Pyrosoma atlanticum). ISME Communications, 1(1). https://doi.org/10.1038/s43705-021-00007-1