Dataset: Eukaryotic and prokaryotic microbial taxa retained by wild-caught doliolids collected during bloom events at three different shelf locations in the northern California Current system in June 2019.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.926299.1Version 1 (2024-04-29)Dataset Type:Other Field Results

Principal Investigator, Contact: Anne W. Thompson (Portland State University)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Project: Collaborative Research: Comparative feeding by gelatinous grazers on microbial prey (Gelatinous Grazer Feeding)


Abstract

Doliolids have a unique ability to impact the marine microbial community through bloom events and high filtration rates. Their predation on large eukaryotic microorganisms is established and evidence of predation on smaller prokaryotic microorganisms is beginning to emerge. We studied the retention of both eukaryotic and prokaryotic microbial taxa by wild-caught doliolids in the northern California Current system. Doliolids were collected during bloom events identified at three different shelf l...

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Samples were collected during daylight from the R/V Atlantis (AGOR-25) along the Newport Hydrographic (NH) Line at station “NH5” (N 44° 39.084’, W 125° 7.151’) on July 22nd as well as along the Trinidad Head (TR) Line at stations “TR1” (N 41° 3.467, W 124° 16.016) and “TR3” (N 41° 3.448, W 124° 26.7) on July 17th and 19th, respectively.

Doliolids were sampled using a modified coupled Multiple Opening and Closing Net and Environmental Sensing System (MOCNESS). Doliolids were rinsed three times with 0.2 µm filtered seawater to remove unattached microbes, collected in a 1-mL sterile bead-beater tube containing 0.55- and 0.25-mm beads, and then frozen at -80 °C.

 


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Results

Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1055560
Portland State University. 16s rRNA from Doliolids from the Northern California Current. 2023/12. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1055560. NCBI:BioProject: PRJNA1055560.

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