Dataset: Collection of subsurface bacteria Nitrospirota and Nitrospinota genome data including IMG and NCBI accessions for sequence datasets in June 2021 (Slow Life in Crust project)

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.933610.1Version 1 (2024-12-29)Dataset Type:Synthesis

Principal Investigator: Beth N. Orcutt (Bigelow Laboratory for Ocean Sciences)

Scientist: Timothy D'Angelo (Bigelow Laboratory for Ocean Sciences)

BCO-DMO Data Manager: Lynne M. Merchant (Woods Hole Oceanographic Institution)


Project: Microbial activity in the crustal deep biosphere (Slow Life in Crust)


Abstract

The phyla Nitrospirota and Nitrospinota have received significant research attention due to their unique mitrogen metabolisms important to biogeochemical and industrial processes. These phyla are common inhabitants of marine and terrestrial subsurface environments and contain members capable of diverse physiologies in addition to nitrite oxidation and complete ammonia oxidation. We used phylogenomics and gene-based analysis with ancestral state reconstruction and gene-tree-species tree reconcili...

Show more

Genomic dataset collection, curation and quality control: This study used publicly available genome assemblies. Existing publicly available genome assemblies were downloaded from the National Center for Biotechnology Investigation (NCBI) and the Integrated Microbial Genomes (IMG) database of the U.S. Department of Energy’s Joint Genome Institute in June 2021. The Genome Taxonomy Database (GTDB) website (release 202) was used to access lists of NCBI assembly accession numbers for the following GTDB-assigned phyla: Nitrospinota, Nitrospinota_A (now called Tectomicrobia), Nitrospinota_B, Nitrospirota, Nitrospirota_A (Leptospirilla). The IMG assemblies were found using the same GTDB taxonomy classifier using the search function on the IMG website. IMG metagenome assemblies that were designated as “public” and “published” were also downloaded for these phyla. Duplicate entries between IMG and NCBI were manually removed.

 


Related Datasets

No Related Datasets

Related Publications

Results

D’Angelo, T., Goordial, J., Lindsay, M. R., McGonigle, J., Booker, A., Moser, D., Stepanauskus, R., & Orcutt, B. N. (2023). Replicated life-history patterns and subsurface origins of the bacterial sister phyla Nitrospirota and Nitrospinota. The ISME Journal, 17(6), 891–902. https://doi.org/10.1038/s41396-023-01397-x
Methods

Grigoriev, I. V., Nordberg, H., Shabalov, I., Aerts, A., Cantor, M., Goodstein, D., Kuo, A., Minovitsky, S., Nikitin, R., Ohm, R. A., Otillar, R., Poliakov, A., Ratnere, I., Riley, R., Smirnova, T., Rokhsar, D., & Dubchak, I. (2011). The Genome Portal of the Department of Energy Joint Genome Institute. Nucleic Acids Research, 40(D1), D26–D32. https://doi.org/10.1093/nar/gkr947
Methods

National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2024 Dec 29]. Available from: https://www.ncbi.nlm.nih.gov/
Methods

Parks, D. H., Chuvochina, M., Rinke, C., Mussig, A. J., Chaumeil, P.-A., & Hugenholtz, P. (2021). GTDB: an ongoing census of bacterial and archaeal diversity through a phylogenetically consistent, rank normalized and complete genome-based taxonomy. Nucleic Acids Research, 50(D1), D785–D794. https://doi.org/10.1093/nar/gkab776
Software

Chaumeil, P.-A., Mussig, A. J., Hugenholtz, P., & Parks, D. H. (2022). GTDB-Tk v2: memory friendly classification with the genome taxonomy database. Bioinformatics, 38(23), 5315–5316. https://doi.org/10.1093/bioinformatics/btac672