Dataset: Field measurements of periostracum cover from focal population

Name: Dataset 3: Field measurements of periostracum cover of mussels from focal population collected at Marshall Gulch Beach, CA in July and August 2022
Published: 2024-08-19
Keywords: periostracum, mussel, microhabitat, oceans

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Spatial Extent: N:38.369738 E:-123.073921 S:38.369738 W:-123.073921

Marshall Gulch, California

Temporal Extent: 2022-07-17 - 2022-08-18

Project:

Invertebrate calcification and behavior in seawater of decoupled carbonate chemistry (OA decoupling)

Principal Investigator:

Brian Gaylord (University of California-Davis BML, UC Davis-BML)

Student:

Alisha Saley (University of California-Davis BML, UC Davis-BML)

BCO-DMO Data Manager:

Lynne M. Merchant (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-08-19

Restricted:

Validated:

Current State:

Preliminary and in progress

Dataset 3: Field measurements of periostracum cover of mussels from focal population collected at Marshall Gulch Beach, CA in July and August 2022

Abstract:

These data build off of experimental incubations described in Dataset 1 and 2. To contextualize laboratory incubations, we measured periostracum cover of live California mussels from multiple microhabitats (relative tidal height and degree of sun exposure) in our focal sample population. This dataset represents periostracum cover measurements of California Mussels conducted at Marshall Gulch Beach, CA in July and August 2022. Dataset 1: Lab incubations of mussels (Mytilus californianus) in 2022 to examine the influence of periostracum cover and pH on external shell dissolution collected at Marshall Gulch Beach, CA from August 2021 to March 2022 (see BCO-DMO related dataset) Dataset 2: Lab incubations of mussels in 2022 to examine the influence of simulated abrasion of periostracum on external shell dissolution collected at Marshall Gulch Beach, CA from August 2021 to March 2022 (see BCO-DMO related dataset)

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Methods & Sampling

To provide ecological context for the laboratory dissolution experiments, we also measured the percent cover of periostracum of living California mussels in the field, focusing on mussels inhabiting different microhabitats within the intertidal zone. We sampled mussels across two tidal heights and three levels of sun exposure on 17 July and 18 August 2022 from the same population where we had collected mussels for the laboratory trials (Marshall Gulch Beach, California). We sampled from the topmost layer of mussels within a 20 cm x 20 cm quadrat positioned within the center of each of 8 mussel beds at each tidal height (characterized as ‘high’ or low’, -0.3 – +0 m sea level height (SLH) and +0.3 - +1 m SLH), haphazardly choosing the first 20 adult individuals within a pre-selected size range (n = 113 mussels total, 54 mm + 4 mm SD). We characterized sun exposure based on the cardinal direction of the mussel bed orientation, which included mussels oriented 200° – 240° SW (termed ‘intense’), 30° – 70° NE (termed ‘average’), and 300° – 340° NW (termed ‘shade’). We photographed both valves of each mussel, ensuring a scale bar was visible in the field of view for use in ImageJ, and averaged the coverage of periostracum across the two valves as the metric of percent cover for a given individual.

Data Processing Description

Data analysis:

We performed computations with R statistical software, RStudio version 2023.06.2. We used a two-way ANOVA to compare average periostracum cover in adult mussels collected from the field at two relative tidal heights and three levels of sun exposure. We omitted the interaction term between the treatments after confirming it failed to explain significant variability in the model. Using tidal height and sun exposure as categorical predictors to explain periostracum cover, we then ran a TukeyHSD test (95% confidence) to identify which microhabitat types differed from one another via post-hoc, pairwise comparisons.

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-2129942

Deployments

None available yet

Instruments

None available yet

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
ID	Shell ID: identification number of individual mussel valve	unitless	sample
species	Mussel species	unitless	species
AphiaID	Unique identifier for the listed taxon in the Aphia database.	unitless	taxon_code
LSID	Life Science Identifier (LSID) for the listed taxon.	unitless	LSID
lat	latitude, South is negative	deicmal degrees	lat
lon	longitude, West is negative	decimal degrees	lon
date_start	collection start date Format: %Y-%m-%d	unitless	date_start
date_end	collection end date Format: %Y-%m-%d	unitless	date_end
light	Microhabitat: sun exposure based on the cardinal direction of the mussel bed orientation, which included mussels oriented 200° – 240° SW (termed ‘intense’), 30° – 70° NE (termed ‘average’), and 300° – 340° NW (termed ‘shade’): N (shady), M (average), or F (intense)	unitless	light_intensity
height	Microhabitat: tidal height: tidal height characterized as ‘high’ (HT) or low’ (LT) based on measured sea level height (-0.3 – +0 m sea level height (SLH) and +0.3 - +1 m SLH)	unitless	tide
peri_percent	Periostracum cover: percent cover of intact periostracum over mussel valve	percentage (%)	no_bcodmo_term
shell_L_avg	Valve length: average value between longest measured length of both mussel valves (ImageJ)	milimeter (mm)	length
shell_A_avg	Shell area: average mussel valve area measurement between both mussel valves (ImageJ)	milimeters squared (mm^2)	area_measured

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Dataset: Field measurements of periostracum cover from focal population