©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Field collection data for taxa detected in Copepod nauplii guts analyzed at San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | HPLC | |
| Taxa detected in field caught nauplii guts analyzed at San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | HPLC | |
| GenBank BLAST results of copepod nauplii gut contents analyzed at San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | HPLC | |
| Identification of copepod gut contents analyzed at San Francisco State University in 2013 (Food Limitation in Copepod nauplii project) | HPLC | |
| Concentrations of acrylate and dimethylsulphoniopropionate from the surface of coral reefs sampled in Moorea, French Polynesia in April 2018 | Shimadzu Prominence high performance liquid chromatography (HPLC) system with a model SPD-20A/V UV-Vis absorbance detector set at 257 nm. | Shimadzu Prominence |
| Biological turnover of acrylate and dimethylsulphoniopropionate from coral reefs sampled in Moorea, French Polynesia in April 2018 | Shimadzu Prominence high performance liquid chromatography (HPLC) system with a model SPD-20A/V UV-Vis absorbance detector set at 257 nm. | Shimadzu Prominence |
| Pigment concentration data from AUV Clio dives conducted during R/V Atlantic Explorer cruise AE1913 in the Sargasso Sea in June of 2019 | Agilent 1100 series high performance liquid chromatography (HPLC) | |
| HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440) | High-Performance Liquid Chromatograph | |
| ChemTax based chl-a of algal groups from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station (BATS) from 2011-2012 (Trophic BATS project) | HPLC analysis was performed using a Shimadzu HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 um) reverse-phase C18 column in series. Absorption spectra and chromatograms (440 ± 4 nm) were acquired using a Shimadzu SPD-M10av photodiode array detector. | High Performance Liquid Chromatograph |
| The global proteome of replete laboratory cultures of Alteromonas macleodii MIT1002 | Data were collected with a Thermo Fusion Orbitrap mass spectrometer interfaced with a Dionex nano-HPLC configured with 2D active modulation chromatography using the methods of McIlvin and Saito 2021. | Dionex nano-HPLC |
| Amino acid flux data collected from the U.S. JGOFS Eqpac Moored Sediment Trap Array in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project | Fluorescence high performance liquid chromatography (HPLC) was used to measure particulate amino acids | High Performance Liquid Chromatography |
| Suspended particle amino acid conc. from MULVFS pump samples from R/V Thomas G. Thompson cruises TT007, TT011 in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project | Fluorescence high performance liquid chromatography (HPLC) was used to measure particulate amino acids | High Performance Liquid Chromatography |
| Amino Acid compositions from plankton tows from R/V Thomas G. Thompson cruises TT007, TT011 in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project | Fluorescence high performance liquid chromatography (HPLC) was used to measure particulate amino acids. | High Performance Liquid Chromatography |
| Amino acid compositions in sediment cores collected during R/V Thomas G. Thompson cruise TT013 in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project | Fluorescence high performance liquid chromatography (HPLC) was used to measure particulate amino acids. | High Performance Liquid Chromatography |
| Apparent Quantum Yields for the Photochemical Formation of Carbonyl Compounds in Seawater from the R/V Endeavor EN589 in the Northwest Atlantic Ocean from September to October 2016. | Shimadzu Prominence high performance liquid chromatography (HPLC) system with a model SPD-20A/V UV-Vis absorbance detector set in dual wavelength mode at 371 and 435 nm. | Shimadzu Prominence high performance liquid chromatography (HPLC) system |
| Porewater geochemistry, including methane, CO2, sulfate, and sulfide from Guaymas Basin hydrothermal sediments collected on R/V Atlantis cruise AT15-56 in Nov-Dec 2009 | Porewater concentrations of dissolved organic acids were measured via High-Pressure Liquid Chromatography (HPLC) after the cruise, using a Beckman Model 332 gradient liquid chromatograph combined with an ISCO V4 UV/VIS detector and a Shimadzu CR3-A integrator. | Beckman Model 332 gradient liquid chromatograph |
| HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected at the Bermuda Atlantic Time-series Study (BATS) site from October 1988 through December 2024 | The HPLC currently in use is an Agilent 1100 series | Agilent 1100 series with diode array detector |
| Cytosolic metallome data of the metalloproteome of Pseudoalteromonas sp. strain BB2-AT2 cultures originally collected from Scripps Pier, California coast in 1995 | Anion exchange chromatography: Agilent 1100 series HPLC. | Agilent 1100 series HPLC |
| Cytosolic metallome data of the metalloproteome of Pseudoalteromonas sp. strain BB2-AT2 cultures originally collected from Scripps Pier, California coast in 1995 | Michrom BioResources, Inc. HPLC system containing a Tosoh Bioscience 5-µm particle size TSKgel G3000SWxl size exclusion column with a guard column. | Michrom BioResources, Inc. HPLC system |
| Metalloproteome protein data of the metalloproteome of Pseudoalteromonas sp. strain BB2-AT2 cultures originally collected from Scripps Pier, California coast in 1995 | Anion exchange chromatography: Agilent 1100 series HPLC. | Agilent 1100 series HPLC |
| Metalloproteome protein data of the metalloproteome of Pseudoalteromonas sp. strain BB2-AT2 cultures originally collected from Scripps Pier, California coast in 1995 | Michrom BioResources, Inc. HPLC system containing a Tosoh Bioscience 5-µm particle size TSKgel G3000SWxl size exclusion column with a guard column. | Michrom BioResources, Inc. HPLC system |
| Survey biogeochemical data from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 | Used for amino acid concentration measurements. | Dionex ICS 5000+ |
| Chemical and biological data from CTD Niskin bottle samples from RVIB Nathaniel B. Palmer NBP1201 in the Ross Sea from 2011-2012 (PRISM-RS project) | High Performance Liquid Chromatograph | HPLC |
| HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected on BATS Validation cruises from June 1996 to July 2024 | The HPLC currently in use is an Agilent 1100 series. | Agilent1100 series HPLC |
| Influence of predator-prey evolutionary history, chemical alarm-cues and feeding selection on induction of toxin production in a marine dinoflagellate | High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). Scanning Fluorescence Detector (Waters 474, Waters, Milford, MA) was used for in-vitro diagnostic testing to analyze compounds of STX and its derivatives. | HPLC system (Waters, Milford, MA) |
| A multi-phylum study of grazer-induced paralytic shellfish toxin production in the dinoflagellate Alexandrium fundyense: A new perspective on control of algal toxicity | High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). Scanning Fluorescence Detector (Waters 474, Waters, Milford, MA) was used for in-vitro diagnostic testing to analyze compounds of STX and its derivatives. | HPLC system (Waters, Milford, MA) |
| Toxin content of Alexandrium catenella in response of nitrogen sources, algal alarm cues, and grazer exposure | High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). Scanning Fluorescence Detector (Waters 474, Waters, Milford, MA) was used for in-vitro diagnostic testing to analyze compounds of STX and its derivatives. | HPLC system (Waters, Milford, MA) |
| Cell-growth gene expression reveals a direct fitness cost of grazer-induced toxin production in red tide dinoflagellate prey | High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). Scanning Fluorescence Detector (Waters 474, Waters, Milford, MA) was used for in-vitro diagnostic testing to analyze compounds of STX and its derivatives. | HPLC system (Waters, Milford, MA) |
| Chlorophyll d15N in Lake Erie collected on NOAA GLERL weekly monitoring cruises from June to October 2017 | Chlorophyll was purified using an Agilent 1200 series HPLC equipped with multi-wavelength UV/Vis detector and two Zorbax C18 columns (dimensions 4.6 × 250 mm, 5 μm). | Agilent 1200 |
| Chlorophyll-a concentrations from CTD cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 cruises in 2013 and 2014 | ||
| Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau | Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. | high-performance liquid chromatography |
| BULA HPLC pigment data from R/V Kilo Moana cruise KM0704 along the transect from Suva, Fiji to Honolulu, Hawaii in April 2007 | ||
| N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements | Liquid chromatographic separations were conducted using a Shimadzu HPLC system (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA) equipped with: system controller (SCL-10A vp), degasser (DGU-20A5), 2 pumps (LC-20AD), autosampler (SIL-20A) with an adjustable injection volume of 0.1-100 μL, and coupled to a Shimadzu automated fraction collector (FRC-20A). | Shimadzu HPLC |
| Ambient concentrations of acetate, glycerol, and mannitol measured from samples collected during R/V Endeavor cruise EN616 in the northwest Atlantic in July 2018 | An Agilent 1200 series HPLC with G1315B diode array detector was used to analyse ATP and ADP concentrations at the end of the 10 min reactions. The HPLC was equipped with a C18 4.6 x 250mm, 5µm reverse-phase column (Waters™), | Agilent 1200 series HPLC with G1315B diode array detector |
| Sample information for metaproteomic samples taken from Station 2 from R/V Knorr KN210-04 in the Western Atlantic Ocean between Uruguay and Barbados from March 2013 | Nano-HPLC configured with 2D active modulation chromatography. | nano-HPLC |
| Sample and treatment information from mass spectrometry assays of metabolite experiments on Thalassiosira pseudonanna 1335 inoculated with three bacterial strains | Mass spectrometry samples were analyzed on a Vanquish UHPLC system (Thermo Fisher Scientific) coupled via heated electrospray ionization (H-ESI) to an ultrahigh resolution tribrid mass spectrometer, the Orbitrap Fusion Lumos (Thermo Fisher Scientific). | Vanquish UHPLC system (Thermo Fisher Scientific) |
| Phytoplankton diagnostic pigments from HPLC from samples collected on R/V Endeavor cruise EN614 in the tropical North Atlantic during May 2018 | The HPLC used for pigment analysis is an Agilent RR1200 with a programmable autoinjector (900 ul syringe head), refrigerated autosampler, degasser, and photo-diode array detector with deuterium and tungsten lamps. | Agilent RR1200 |
| Large substrate hydrolysis of bulk water samples taken aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic. | HPLC system with Hitachi fluorescence detectors (L-7485, L-2485, Chromaster - 5440) | HPLC system with Hitachi fluorescence detec |
| Phytoplankton diagnostic pigments from HPLC from samples collected on R/V Endeavor cruise EN640 in the tropical North Atlantic during June-July 2019 | The HPLC used for pigment analysis is an Agilent RR1200 with a programmable autoinjector (900 ul syringe head), refrigerated autosampler, degasser, and photo-diode array detector with deuterium and tungsten lamps. | Agilent RR1200 |
| HPLC data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic, offshore from North Carolina, USA, in 2021 and 2022 | High performance liquid chromatography (HLPC) coupled to an in-line photodiode array spectrophotometer (Jeffrey et al. 1997). Study also used a Shimadzu SPD-M10AV photodiode array detector. | |
| Phytoplankton carbon biomass by taxa from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018 | Automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. | HPLC |
| Pigment data by phytoplankton taxa from CTD casts from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018 | Automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. | HPLC |
| Pigment concentrations determined by HPLC from samples collected on Leg 1 (Seattle, WA to Hilo, HI) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1814) on R/V Roger Revelle from September to October 2018 | Samples are analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Samples are extracted and analyzed on a Waters 996 absorbance photodiode array detector in combination with a Waters 2475 fluorescence detector in September 2019 at OSU. | HPLC |
| Pigment concentrations determined by HPLC from samples collected on Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from Oct-Nov 2018 | Samples are analyzed on a Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector. Samples are extracted and analyzed on a Waters 996 absorbance photodiode array detector in combination with a Waters 2475 fluorescence detector in September 2019 at OSU. | HPLC |
| Iron ligand concentration in particles (1-51 μm) analyzed by liquid chromatography-mass spectrometry from samples collected on the US GEOTRACES Pacific Meridional Transect (PMT) cruise RR1814 (GP15) on R/V Roger Revelle in October 2018 | Dionex Ultimate 3000 | |
| Iron ligand speciation profiles analyzed by liquid chromatography-mass spectrometry from samples collected on the US GEOTRACES Pacific Meridional Transect (PMT) cruises (GP15, RR1814 & RR1815) on R/V Roger Revelle from September to November 2018 | Dionex Ultimate 3000 | |
| Phytoplankton pigment concentrations for samples collected from the upper 200 meters during the GP17-OCE cruise on R/V Roger Revelle (RR2214) in the South Pacific and Southern Oceans from December 2022 to January 2023 | Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector | Waters Alliance 2690 HPLC Separations Module |
| Dissolved cysteine and glutathione from R/V Knorr KN199-04 in the subtropical North Atlantic Ocean from October 2010 (U.S. GEOTRACES NAT project) | Agilent 1200 HPLC | High Performance Liquid Chromatograph |
| Fluorometer data (volts) from CTD casts from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018 | Automated 1100 HPLC system with Agilent temperature-controlled autosampler, Peltier temperature-controlled column oven compartment, PDA detector and ChemStation software. | HPLC |
| Niskin bottle water samples and associated CTD measurements from the Hawaii Ocean Time-Series cruises from 1988-2023 | Chlorophyll a and photosynthetic accessory pigments were measured by high performance liquid chromatography (HPLC) according to Wright et al. (1991). | HPLC |
| Metaproteomics analyses of mixed-layer water from Hawaii Ocean Time-series Station ALOHA, collected on R/V Kilo Moana cruise KM2204 in March 2022, and incubated with 15N2 to track biosynthetic incorporation by diazotrophic microbes | Dionex UltiMate 3000 RSLCnano w/ GL Sciences Monocap Ultra monolithic capillary C18 column with nanoelectrospray ionization (Proxeon Nanospray Flex source) | Dionex Ultimate 3000 LC system |
| Retinoid data from Niskin bottle samples from B/O Sarmiento de Gamboa cruise Hotmix2014 in the Mediterranean in 2014 (Marine Retinoids project) | For analysis, samples were extracted with methanol (retinal, chla, and Bchl) and with hydroxylamine (oxime) and analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The LCMS system consists of a ThermoTSQ Quantum Access electro-spray ionization triple quadrupole mass spectrometer, coupled to a Thermo Accela High Speed Liquid Chromatography system | Thermo Accela High Speed Liquid Chromatography system |
| Shimadzu HPLC system with a reverse phase column (Agilent Eclipse XDB-C8, 3.5 micron particle size, 150 mm length x 4.6 mm diameter), with photodiode array (PDA) detector set as 450 nm. | Shimadzu HPLC system | |
| High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico | Agilent RR1200 HPLC controlled bu Agilent Chemstation software | Agilent RR1200 HPLC |
| Results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project) | Used in HPLC Analysis | Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop |
| Results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project) | Used in HPLC analysis | Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range) |
| Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project) | Used in HPLC Analysis | Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop |
| Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project) | Used in HPLC analysis | Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range) |
| Pigment concentrations derived from High-Performance Liquid Chromatography (HPLC) analysis from samples collected during the Tara Pacific expedition from 2016-2018 | Instrument calibration is done once per year using standards from DHI Water and Environment (Denmark) and Sigma Aldrich (Chla and Chlb). Preventive maintenance of the instrument is done once per year (seal replacements, window detectors, injection valve, and worn parts). Long-term quality control of the instrument's performance is done by injecting mixed pigment standards from DHI several times a year. | Aligent 1200 Series Gradient HPLC System |
| HPLC pigment analyses of CTD-collected samples from R/V Knorr cruise KN207-03 in the North Atlantic (transect from Ponta Delgada, Azores to Reykjavik, Iceland) in 2012 (NA-VICE project) | Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). | HPLC |
| HPLC pigment data from samples collected during R/V Savannah cruises conducted in the South Atlantic Bight off the coast of Georgia from 2015-2017 | High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array, and fluorescence detectors. | High Performance Liquid Chromatograph (HPLC) Agilent 1100 |
| Sample HPLC pigments from RVIB Nathaniel B. Palmer NBP1302 cruise in the Ross Sea during 2013 (TRACERS project) | Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). | HPLC |
| Underway HPLC pigments from the RVIB Nathaniel B. Palmer NBP1302 cruise in the Ross Sea during 2013 (TRACERS project) | Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100). | HPLC |
| Pigments from HPLC analysis of bottle samples collected aboard Oceanus from R/V Oceanus OC404-01, OC404-04, OC415-01, OC415-03 in the Sargasso Sea from 2004-2005 (EDDIES project) | High Performance Liquid Chromatograph | |
| Time series HPLC pigment data as measured by the Bermuda Biological Research Station (BBRS) from B/O Hermano Gines cruises CAR-002 to CAR-030 in the CARIACO basin from 1995-12-13 through 1998-04-21 (CARIACO Ocean Time-Series Program) | High Performance Liquid Chromatography | High Performance Liquid Chromatography |
| Time series HPLC pigment data as measured by Horn Point Laboratory (HPL) from B/O Hermano Gines cruises CAR-124 to CAR-175 in the CARIACO basin from 2006-07-04 to 2010-12-08 (CARIACO Ocean Time-Series Program) | High Performance Liquid Chromatography | |
| Time series HPLC pigment data as measured by MOTE Marine Laboratory & Aquarium from B/O Hermano Gines cruises CAR-031 to CAR-068 in the CARIACO basin from 1998-06-09 to 2001-07-10 (CARIACO Ocean Time-Series Program) | High Performance Liquid Chromatography | High Performance Liquid Chromatography |
| Time series HPLC pigment data as measured by the NASA Goddard Space Flight Center from B/O Hermano Gines cruises CAR-176 to CAR-232 in the CARIACO basin from 2011-01-11 through 2017-01-12 (CARIACO Ocean Time-Series Program) | High Performance Liquid Chromatography | High Performance Liquid Chromatography |
| Diagnostic accessory pigment data analyzed by HPLC from samples collected on R/V Challenger cruise LIS0507 in the Long Island Sound in 2007 | HPLC pigment data was processed using CHEMTAX software. | High Performance Liquid Chromatograph |
| Bottle sample data from CTD casts from R/V Hugh R. Sharp HRS1610 in the Mid-Atlantic coastal waters from August 2016 (CyanateInTheSea project) | A high-performance liquid chromatography was used for cyanate analysis | High-performance liquid chromatography (Shimadzu) |
| Hurricane Harvey impacts on biogeochemistry of sediments assessed using samples collected in Mission-Aransas Estuary in south Texas from June 2017 to March 2019 | Shimadzu HPLC with reverse phase column was used for pigment analysis. Reverse phase column was Agilent Eclipse XDB-C8, 3.5 µm particle size, 150-mm length × 4.6-mm diameter | Shimadzu HPLC system |
| Hurricane Harvey impacts on biogeochemistry of water assessed using samples collected in Mission-Aransas Estuary in south Texas from June 2017 to March 2019 | Shimadzu HPLC with reverse phase column was used for pigment analysis. Reverse phase column was Agilent Eclipse XDB-C8, 3.5 µm particle size, 150-mm length × 4.6-mm diameter | Shimadzu HPLC system |
| Supplementary Table 3A: Concentration of archaeal and bacterial lipid biomarkers, ratio of core vs intact polar lipids and average chain length of bacterial dietherglycerol lipids. | All membrane lipid analyses were performed at MARUM (Bremen, Germany) with an ultra-high-pressure liquid chromatography (UHPLC) coupled to mass spectrometry (MS) on a Dionex Ultimate 3000RS UHPLC connected to an ABSciEX QTRAP4500 Triple Quadrupole/Ion Trap MS (UHPLC-Triple Quad-MS) via a Turbolon electrospray ion source (ESI) | |
| Relative abundances of the diether lipid archaeol and tetraether lipids with zero, one, two and three rings and crenarchaeol from rock samples at different depths. | All membrane lipid analyses were performed at MARUM (Bremen, Germany) with an ultra-high-pressure liquid chromatography (UHPLC) coupled to mass spectrometry (MS) on a Dionex Ultimate 3000RS UHPLC connected to an ABSciEX QTRAP4500 Triple Quadrupole/Ion Trap MS (UHPLC-Triple Quad-MS) via a Turbolon electrospray ion source (ESI). | ultra-high-pressure liquid chromatography (UHPLC) |
| Diversity of archaeal intact polar lipids (IPLs) and changes in relative abundance of bacterial dietherglycerol lipids (DEGs) within the selected 11 samples at different depths | All membrane lipid analyses were performed at MARUM (Bremen, Germany) with an ultra-high-pressure liquid chromatography (UHPLC) coupled to mass spectrometry (MS) on a Dionex Ultimate 3000RS UHPLC connected to an ABSciEX QTRAP4500 Triple Quadrupole/Ion Trap MS (UHPLC-Triple Quad-MS) via a Turbolon electrospray ion source (ESI). | ultra-high-pressure liquid chromatography (UHPLC) |
| Archaeal core lipids, intact polar lipids (IPLs), and bacterial dietherglycerol (DEG) lipids in the drilling mud, subsampled from whole round core 29R3 and selected rock samples. | All membrane lipid analyses were performed at MARUM (Bremen, Germany) with an ultra-high-pressure liquid chromatography (UHPLC) coupled to mass spectrometry (MS) on a Dionex Ultimate 3000RS UHPLC connected to an ABSciEX QTRAP4500 Triple Quadrupole/Ion Trap MS (UHPLC-Triple Quad-MS) via a Turbolon electrospray ion source (ESI). | ultra-high-pressure liquid chromatography (UHPLC) |
| An ocean productivity database using consistent, globally-acquired data from in situ primary productivity based on 14C assimilation, and including ancillary data, 1985-2008 | Used to measure pigments | |
| Biological oceanographic measurements, 16S rRNA gene amplicons and metagenomes from surface seawater taken from August 2017 to June 2021 at sites within and adjacent to Kāneʻohe Bay, Oʻahu, Hawaiʻi | Photosynthetic pigments measured via high performance liquid chromatography: Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector, | Waters 2690 separations module equipped with a C18 column and full spectrum photodiode array detector |
| MS-based lipidomics on diatom response to allelopathic chemicals from the red tide dinoflagellate (Karenia brevis) | Waters Acquity UPLC quaternary solvent manager system fitted with a Waters ACQUITY UPLC BEH C18 column | |
| Pigment concentration data for mesocosm incubation experiment simulating a phytoplankton bloom in Chesapeake Bay August 2021 | Pigment samples were analyzed by High Performance Liquid Chromatography (HPLC) (Pinckney et al., 1996, 2001, 2010). | High Performance Liquid Chromatography (HPLC) |
| Sugar concentrations from the BATS site in the Sargasso Sea, 2001-2004 (Ocean Microbial Observatory project) | DCNS were analyzed via pulsed amperometric detection highperformance liquid chromatography (PAD-HPLC) using a Dionex (Sunnyvale, CA) Bio-LC600 equipped with a GS-50 pump, ED-50 detector, and AS-50 autosampler. Chromeleon 6.2 integration software was used for data integration. | PAD-HPLC |
| N isotopic composition of Phenylalanine and Glutamic Acid from a number of organisms, demonstrating new HPLC protocol for precise isotopic measurements | Liquid chromatographic separations were conducted using a Shimadzu HPLC system (Shimadzu Scientific Instruments, Inc., Columbia, MD, USA) equipped with: system controller (SCL-10A vp), degasser (DGU-20A5), 2 pumps (LC-20AD), autosampler (SIL-20A) with an adjustable injection volume of 0.1-100 μL, and coupled to a Shimadzu automated fraction collector (FRC-20A). | Shimadzu HPLC |
| Chemical fingerprints of Montipora capitata aqueous extracts collected in Kaneohe Bay, Oahu, Hawaii during 2014 | Waters Alliance® 2695 High Performance Liquid Chromatography (HPLC) system (Phenomenex Syneri Hydro RP 4 um column; 250 x 4.6 mm; linear gradient from 100% water+0.1% acetic acid to 100% methanol+0.1% acetic acid) interfaced with a Waters 2998 Photodiode Array Detector. | HPLC |
| Biological, chemical, and physical water quality indicators of the Neuse River, North Carolina from 2008 through 2013 | Two hundred microliters of extractant from each vial was injected into the HPLC system using a Spectra Physics (now Thermo Separations Products) AS3000 autosampler and SP8800 pump, running a non-linear, 55 minute, 2-solvent gradient adapted from Van Heukelem et.al. 1994 or 1995?. | HPLC system |
| Benthic chamber geochemical data obtained with an in situ benthic lander from the R/V Savannah at sampling stations across the Louisiana shelf and slope in the Northern Gulf of Mexico during four cruise between July 2021 and July 2022 | HPLC measurements were conducted with a Waters 1525 binary pump with degasser, coupled to a Waters 2487 dual absorbance detector, and Waters 2707 Autosampler. | HPLC |
| Time-series Niskin-bottle sample data from R/V Hermano Gines cruises in the Cariaco Basin from 1995 through 2017 (CARIACO Ocean Time-Series Program) | HPLC analysis was restarted in July 2006 (CAR-123). Samples were filtered 47 mm Whatman GF/F filters at 8 depths (1, 7, 15, 25, 35, 55, 75 and 100m). The volume filtered depends on the amount of particles in the water. Replicates were taken at the 1m depth. Filters were stored in aluminum envelopes and stored in the fridge until reaching shore. Once on shore, samples were stored at -40°C until transportation to the US. Horn Point Laboratory (http://www.hpl.umces.edu/) performs the analyses through a collaborative agreement with NASA. The method used was Van Heukelem and Thomas (2001). | HPLC |
| Global proteome analyses of the nitrite-oxidizing bacterium Nitrospira marina grown under atmospheric and low oxygen concentrations | Dionex Ultimate nanoLC system | |
| Nutrients, targeted proteomics, and pigments from the METZYME cruise (KM1128) | Chromatographic separation and mass spectrometry were performed using a Paradigm MS4 HPLC (Michrom Bioresources) coupled to a Thermo Vantage TSQ mass spectrometer (Thermo Scientific). Pigments were determined using an Agilent 1100 HPLC (High Performance Liquid Chromatography) system with diode array and fluorescence detection. | Paradigm MS4 HPLC |
| Dissolved inorganic nutrient concentrations from ctd cast deployments and underway seawater inflow from Endeavor 532 and Endeavor 538 | ||
| Cluster (combined temperature, nutrient concentration, and CO2) results from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022 | Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to a SCIEX 4500 QTRAP mass spectrometer (AB Sciex, Framingham, MA, USA) | Prominence UFLC system (Shimadzu, Kyoto, Japan) |
| CO2 experiment physiology and carbonate chemistry from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022 | Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to a SCIEX 4500 QTRAP mass spectrometer (AB Sciex, Framingham, MA, USA) | Prominence UFLC system (Shimadzu, Kyoto, Japan) |
| N:P ratio experiment physiology and carbonate chemistry laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022 | Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to a SCIEX 4500 QTRAP mass spectrometer (AB Sciex, Framingham, MA, USA) | Prominence UFLC system (Shimadzu, Kyoto, Japan) |
| Single-factor temperature experiment physiology and carbonate chemistry from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022 | Prominence UFLC system (Shimadzu, Kyoto, Japan) coupled to a SCIEX 4500 QTRAP mass spectrometer (AB Sciex, Framingham, MA, USA) | Prominence UFLC system (Shimadzu, Kyoto, Japan) |
| Particulate vitamin B12 profiles from R/V Kilo Moana KM1314 in the N Pacific Ocean (Seattle to Honolulu, Line P, Station ALOHA), Aug-Sept 2013 (Nitrification and Planktonic Biodiversity project) | HPLC (Agilent 1100) and UPLC Waters Acquity | |
| Photochemical production rates of acrylate in seawater following exposure to sunlight from a variety of marine environments between 2011-2018. | Shimadzu Prominence high performance liquid chromatography (HPLC) system with a model SPD-20A/V UV-Vis absorbance detector set at 257 nm. | Shimadzu Prominence |
| Photosynthetic pigments from sea ice samples collected on R/V Nathaniel B. Palmer cruise NBP1910 along the Western Antarctic Peninsula from November to December 2019 | ||
| Phytoplankton growth and grazing mortality from HPLC pigments sampled in the Gulf of Mexico on R/V Nancy Foster cruises in May 2017 and May 2018 | Agilent 1100 HPLC system with temperature-controlled autosampler, Peltier temperature-controlled column oven compartment and PDA detector. | Agilent 1100 HPLC system |
| Phytoplankton HPLC pigment concentrations from samples collected in the Gulf of Mexico on R/V Nancy Foster cruises in May 2017 and May 2018 | Agilent 1100 HPLC system with temperature-controlled autosampler, Peltier temperature-controlled column oven compartment and PDA detector. | Agilent 1100 HPLC system |
| CTD data, bottle nutrients, and chlorophyll-a from phytoplankton taxa from R/V Pelican PE08-54, PE09-12, PE10-01 in the Northwest Florida shelf off Panama City FL. from 2008-2009 (BenDiM project) | High Performance Liquid Chromatograph | |
| Physicochemical and phytoplankton data collected during the STRATIPHYT I and II cruises in the Northeast Atlantic Ocean. Cruises took place during the summer of 2009 and spring of 2011 aboard the R/V Pelagia. | HPLC (Waters 2695 separation module, 996 photodiode array detector) equipped with a Zorbax Eclipse XDB-C8 3.5 micrometer column (Agilent Technologies) | Waters 2695 separation module, 996 photodiode array detector |
| Phytoplankton data from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project) | An automated Hewlett Packard 1100 HPLC system was used to separate taxon-specific pigments. | HPLC |
| Pigment concentrations (HPLC) from R/V Atlantic Explorer cruises AE1102, AE1118, AE1206, AE1219 in the Sargasso Sea, Bermuda Atlantic Time-Series Station (BATS) from 2011-2012 (Trophic BATS project) | HPLC analysis was performed using a Shimadzu HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 um) reverse-phase C18 column in series. Absorption spectra and chromatograms (440 ± 4 nm) were acquired using a Shimadzu SPD-M10av photodiode array detector. | High Performance Liquid Chromatograph |
| Algal pigment concentrations measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018. | High Performance Liquid Chromatograph (HPLC) Agilent 1100 equipped with autosampler, photodiode array and fluorescence detectors. | Agilent 1100 |
| Algal pigment concentrations as measured by HPLC from RVIB Nathaniel B. Palmer cruises in the Ross Sea Southern Ocean from 2005 to 2006 (CORSACS project) | HP 1050 HPLC system equipped with autosampler, photodiode array and fluorescence detectors | High Performance Liquid Chromatograph |
| Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-15-18 at the Cape Lookout Continental Shelf and Slope during July 2015 | SO42- was measured by non-suppressed HPLC with UV detection. | HPLC |
| Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-16-16 at the Cape Lookout Continental Shelf and Slope during May 2016 | SO₄²⁻ was measured by non-suppressed HPLC with UV detection | HPLC |
| Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-17-15 in the Gulf of Mexico during July and August 2017 | Br⁻, NO₂⁻, NO₃⁻, and SO₄²⁻ were measured by non-suppressed HPLC with UV detection | HPLC |
| Results from experiments on Pseudo-nitzschia fraudulenta growth and domic acid productionunder varying CO2 and silicate levels (PhytoTM_in_HighCO2 project) | High performance liquid chromatography with ultraviolet detection (HPLC-UV) of domoic acid was performed using a Shimadzu SCL-10ADVP controlled system. | High Performance Liquid Chromatograph |
| The global proteome of replete laboratory cultures of Ruergeria pomeroyi DSS-3 | Data were collected with a Thermo Fusion Orbitrap mass spectrometer interfaced with a Dionex nano-HPLC configured with 2D active modulation chromatography using the methods of McIlvin and Saito 2021. | Dionex nano-HPLC |
| Dissolved black carbon (DBC) concentrations and related data from water samples collected between 2011 and 2019 in the Pacific and Atlantic Oceans and from rivers in North America, South America, Africa, and Russia | Dionex Ultimate 3000 HPLC connected to a Delta V IRMS via an LC Isolink interface – To measure BPCA-specific stable carbon isotopic values of DBC for all samples. | Dionex Ultimate 3000 HPLC |
| Normalized protein abundance data and protein annotations for proteomic data from laboratory cultures of Ruegeria pomeroyi DSS-3 and Alteromonas macleodii MIT1002 in 2022 | Liquid chromatography-mass spectrometry (LC-MS) (Michrom Advance HPLC coupled to a Thermo Scientific Fusion Orbitrap mass spectrometer with a Thermo Flex source) | Michrom Advance HPLC |
| Record of abundance and δ2H of dinosterol in down core lake sediments from T Lake, Palau collected in September 2013 | 1100 HPLC system (Agilent). High performance liquid chromatograph, equipped with fraction collector for preparative separations. | 1100 HPLC system (Agilent) |
| Trichodesmium nutrient-limited proteomes in 380 and 750 uatm CO2 from experiments conducted at the University of Southern California from 2011-2013 (HiCO2_AdaptCyano project) | Michrom Bioresources. And a 100 μm inner diameter 15 cm long capillary column with a pulled tip (packed in-lab with MAGIC C18AQ 200 Å pore size 3 μm particle size from Michrom Bioresources). | Michrom Advance nanoflow LC and autosampler |
| Underway data including temperature, salinity, fluorometry, pigments from RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea Southern Ocean (CORSACS project) | HP 1050 HPLC system equipped with autosampler, photodiode array and fluorescence detectors | High Performance Liquid Chromatograph |
| Dissolved B vitamins from Hotmix expedition, B/O Sarmiento de Gamboa Hotmix, May 2014 | Thermo Accela High Speed Liquid Chromatography system | |
| Particulate B vitamins from Hotmix expedition, B/O Sarmiento de Gamboa Hotmix, May 2014 | Thermo Accela High Speed Liquid Chromatography system | |
| Particulate B vitamins from Vitacopss, San Pedro Ocean Time series (SPOT), 2015 from R/V Yellowfin near Los Angeles, California from 2015-2016 (B-vitamin plankton succession project) | Thermo Accela High Speed Liquid Chromatography system | |
| Wavelength- and temperature-dependent apparent quantum yields (AQYs) for the photochemical production of acrylate in seawater | Acrylate quantification: A Shimadzu high performance liquid chromatography system with a model SPD-20A UV-Vis absorbance detector. | Shimadzu high performance liquid chromatography |
©2020 Biological and Chemical Oceanography Data Management Office.