Instrument: Hemocytometer

Acronym: Hemocytometer
External Identifier:

Description

A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from:
http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.


Dataset NamePI-Supplied DescriptionPI-Supplied Name
Fecundity assessment of Acropora cervicornis colonies from spawning observations and gamete bundle analysis in August 2020 at Mote Marine Laboratory Replicate sperm counts were recorded using a hemocytometer Neubauer hemocytometer
Fecundity and number of oocytes from Acropora cervicornis genotypes measured July 2020 at Mote Marine LabNeubauer hemocytometer
Reproductive histology and energetics in Acropora hyacinthus in response to the 2019 Moorea bleaching event.
Results from 14C-labeled uptake experiments determining uptake of specific dissolved organic compounds which showed high potential for osmotrophyhaemocytometer
Cell counts of Breviolum antillogorgium cultures isolated from the octocoral Antillogorgia bipinnata by sample and treatment for cultures historically grown at 26C and 30C and reciprocally grown at 26C and 30C, Oct - Dec 2018Used to make cell counts. Reichert Brightline hemocytometer
Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012) from Taiwan, 2010 (Cumbo, 2012) (MCR LTER project, Climate_Coral_Larvae project)Hemocytometer
Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)Hemocytometer
Symbiont Symbiodinium density in brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)Hemocytometer
Cell counts in newly settled polyps of Antillogorgia bipinnata inoculated with one of six genotypes of Breviolum antillogorgium and reared at 26 and 30 degrees CelsiusUsed to make cell counts. Reichert Brightline hemocytometer
Experimental results: coral calcification, chlorophyll-a content, algal density, energy reserves, and tissue biomass from samples of reef systems collected from northwest Fiji in 2011Endosymbiont quantification was calculated using 6 independent replicate counts on a hemocytometer, using a Nikon microphot-FXA epifluorescent microscope at 100X magnification. Hemocytometer
Coral fragment surface area calculations utilizing two methods (tin foil and Image J) and corresponding zooxanthellae count dataMarienfeld Superior Neubauer Improved Chamber
Results of laboratory experiment examining growth, CO2- and N2-fixation of Crocosphaera watsonii in differing pCO2 and light levels; conducted in the Hutchins Laboratory, USCCulture cell density was determined using a haemocytometer and an Olympus BX51 microscope. Hemocytometer
Results of laboratory experiment examining growth and N2-fixation of Crocosphaera watsonii under differing pCO2 levels; conducted in the Hutchins Laboratory, USCCulture cell density was determined using a haemocytometer and an Olympus BX51 microscope. Hemocytometer
Results of laboratory experiment examining growth, CO2- and N2-fixation of Crocosphaera watsonii isolates in differing light intensities; conducted in the Hutchins Laboratory, USCCulture cell density was determined using a haemocytometer and an Olympus BX51 microscope. Hemocytometer
Results from experiments examining the cell diameter of Crocosphaera watsonii (WH0003) in response to light irradiance; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer at the end of each dilution period. Hemocytometer
Results from experiments examining the cell diameter of Crocosphaera watsonii (WH0003) as a function of total P, light, and CO2; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer. Hemocytometer
Results of experiments examining cellular growth by Crocosphaera watsonii (WH0003) under differing levels of phosphate, light, and pCO2; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer at the end of each dilution period. Hemocytometer
Results from experiments examining efficiency of cellular P for CO2- and gross N2-fixation rates by Crocosphaera watsonii (WH0003) as a function of light and pCO2; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer. Hemocytometer
Results of experiments examining CO2 and gross N2 fixation rates by Crocosphaera watsonii (WH0003) under differing levels of phosphate, light, and pCO2; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer. Hemocytometer
Results from experiments examining cellular growth, CO2- and N2-fixation by Crocosphaera watsonii (WH0003) as a function of light; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer at the end of each dilution period. Hemocytometer
Results of experiments examining phosphate (P) uptake rates and cellular P content of Crocosphaera watsonii from WH0003 region of the genome under differing levels of phosphate, light, and CO2; conducted in the Hutchins Laboratory, USCCells were counted microscopically in each replicate culture with a hemocytometer. Hemocytometer
DEPRECATED: Optical properties of Orbicella faveoalta fragments (chl-a, Symbiodinium density, absorbance, and absorbance efficiency) from Rosaria and Varadero reef sites, Colombia, 2016 and 2017 (Varadero Reef project)Used for cell counting phase hemocytometer (Hausser Scientific, Horsham, USA)
Experimental results: Exopolymer and carbohydrate production by diatoms with growth; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project)Counts of 400 cells from each culture were made using a hemocytometer (Fuchs-Rosenthal ruling, Hauser Scientific) (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope. Hemocytometer
Coral physiology parameters acquired during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.Hausser Scientific Bright-Line Counting Chamber
Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)Used along with fluorescence microscope to quantify microbial densities. Improved Neubauer hemocytometer
Kelp responses to temperature at 5-10 meters depth at three locations along the California coast from September to December 2016
Time series of T. suecica densities under fluctuating temperatures experiment from May 2023 to Aug 2023This size range was experimentally determined by photographing cells on a Hemacytometer and analyzing size with ImageJ.  Hemacytometer
Data on how nutrient and sediment loading affect coral functionality in a tropical branching coralImproved Neubauer Haemocytometer
Experimental results: Exopolymer production by phytoplankton under oxidative stress; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project)Counts of 400 cells from each culture were made using hemocytometers (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope. Hemocytometer
Coral biometrics data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018Algal cells were counted with a Neubauer hemocytometer on a light microscope (Fisher Scientific). Neubauer hemocytometer
Lab experiment to determine survival and mortality of Ceraesignum (formerly Dendropoma) maximum after 3, 6, 9, 12, 15 and 18 days depending on food in Moorea, French Polynesia (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Sizes of Ceraesignum maximum larvae in lab experiment after 3, 6, 9, 12, 15 and 18 days depending on food in Moorea, French Polynesia from September to October 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Settlement challenge experiment after 10 days post hatch in Moorea, French Polynesia from September to October 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Settlement challenge experiment after 18 days post hatch in Moorea, French Polynesia from September to October 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Lab experiment to determine survival and mortality of Ceraesignum (formerly Dendropoma) maximum after 3, 6, and 9, days depending on amount fed in Moorea, French Polynesia from October 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Experiment 2: Settlement challenge experiment after 6 days post hatch in Moorea, French Polynesia from October 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Experiment 2: Settlement challenge experiment after 8 days post hatch in Moorea, French Polynesia from October, 2009 (Vermetids_Corals project)Investigators used a hemocytometer to count algal cells and calculate densities of phytoplankton stocks and amount of stock to add to containers for each treatment. hemocytometer
Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature, and bacteria - Aggregation Phase - Carbonate System + TEP from UCSB MSI Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)Diatom cell abundance was monitored daily by counting cells in a Sedgwick-Rafter Cell S50 (SPI Supplies, West Chester, PA, USA) using an inverted Axiovert 200 microscope (Zeiss, Jena, Germany). Sedgwick-Rafter Cell S50
Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria - Aggregation Phase - Sinking Velocity from a from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)Diatom cell abundance was monitored daily by counting cells in a Sedgwick-Rafter Cell S50 (SPI Supplies, West Chester, PA, USA) using an inverted Axiovert 200 microscope (Zeiss, Jena, Germany). Sedgwick-Rafter Cell S50
Cell counts from an experiment examining the effect of Sr on the coccolithophore Scyphosphaera apsteinii calcificationCells were counted on each collection day using a hemocytometer or Sedgwick-Rafter chamber. hemocytometer
Laboratory results on pre and post bleach symbiont density in Porites divaricata collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)Hemocytometer
Results from growth rate experiment with the diatom Thalassiosira wessiflogii in semi-continuous culture; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project)Counts of 400 cells from each replicate culture were made by light microscopy using a hemocytometer (Fuchs-Rosenthal ruling, Hauser Scientific). Hemocytometer
Nutrient transfer experiments with host coral and symbionts under varying environmental conditions conducted March 2014 and March 2015Algal densities were quantified from replicate haemocytometer counts (AO Spencer Bright Line Improved Neubauer haemocytometer) AO Spencer Bright Line Improved Neubauer haemocytometer
Urchin Fertilization Studies from Levin laboratory at Scripps Institute of Oceanography from 2009 to 2012 (SeapHOx project)Sperm were collected dry from the gonopores of males and placed in a small vial and kept on ice until use. A 0.1% dilution from dry sperm was made to verify sperm motility, and then preserved in formalin to count sperm densities with a hemacytometer in order to calculate the amount of diluted sperm required to attain desired sperm-egg ratios and sperm densities for each treatment. Hemocytometer