Dataset: Phytoplankton physiology

Name: Physiology of phytoplankton collected in coastal waters at Palmer LTER near Palmer Station, Antarctica, 2012 and 2013 (Phytoplankton Seasonal Dynamics project)
Published: 2016-10-19
Keywords: oceans

Cite This Dataset

Spatial Extent: N:-64.7795 E:-64.0725 S:-64.7795 W:-64.0725

Project:

The seasonal dynamics of CO2, primary production, and DMS in the Western Antarctic Peninsula: Measurements of pools and processes using mass spectrometry (Phytoplankton Seasonal Dynamics)

Lead Principal Investigator:

Dr Francois Morel (Princeton University)

Principal Investigator:

John Dacey (Woods Hole Oceanographic Institution, WHOI)

Contact:

Jodi N. Young (Princeton University)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version Date:

2016-10-19

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Physiology of phytoplankton collected in coastal waters at Palmer LTER near Palmer Station, Antarctica, 2012 and 2013 (Phytoplankton Seasonal Dynamics project)

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Methods & Sampling

References:

Detailed description of methods are given in the published peer-reviewed papers listed below:

Goldman, J., Kranz, S., Young, J.N., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Gross and net production during the spring bloom along the Western Antarctic Peninsula New Phytologist 205 (1): 182-191. DOI: 10.1111/nph.13125.

Kranz, S., Young, J.N., Goldman, J., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Low temperature reduces the energetic requirement for the CO2 concentrating mechanism in diatoms. New Phytologist 205 (1): 192-201. DOI: 10.1111/nph.12976

Tortell, P.D., Asher, E.C., Dacey, J.W.H. Kranz, S., Young, J.N., Goldman, J. Ducklow, H., Grzymski, J. Stanley, R., Morel, F.M.M. (2014) Metabolic balance of coastal Antarctic waters revealed by autonomous high frequency pCO2 and dO2 /Ar measurements Geophysical Research Letters DOI: 10.1002/2014GL061266

Young, J.N., Goldman, J., Kranz, S., Tortell, P.D., Morel, F.M.M. (2015) Slow carboxylation of Rubisco constrains the rate of carbon fixation during Antarctic phytoplankton blooms. New Phytologist 205 (1): 172-181. DOI: 10.1111/nph.13021

Data Processing Description

BCO-DMO Processing:
- added conventional header with dataset name, PI name, version date, reference information
- column names reformatted to comply with BCO-DMO standards
- added lat and lon for mapping purposes
- replaced n.d. with nd (no data) and blank cells with ND
- reformatted date from Month d, yyyy to yyyy-mm-dd

Data Files

File
phyto_physiology.csv (Comma Separated Values (.csv), 8.51 KB) MD5:fb32d91b74751498d42084a15f191f4e Primary data file for dataset ID 662036

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Office of Polar Programs (formerly NSF PLR)	PLR-1043593

Deployments

Deployment	Synonyms	Start Date	Platform	Investigator
Morel_PAL-LTER		2012-10-24	Palmer LTER	Dr Francois Morel (Principal Investigator)	data info

Instruments

CTD Sea-Bird SBE SEACAT 19plus

Supplied Name:

Supplied Description:

Depth profiles of temperature and salinity measurements

Instrument Type

Generic Name: CTD Sea-Bird SBE SEACAT 19plus

Acronym: Sea-Bird SEACAT 19 Plus

Generic Description:

Self contained self powered CTD profiler. Measures conductivity, temperature and pressure in both profiling (samples at 4 scans/sec) and moored (sample rates of once every 5 seconds to once every 9 hours) mode. Available in plastic or titanium housing with depth ranges of 600m and 7000m respectively. Minature submersible pump provides water to conductivity cell.

Membrane Inlet Mass Spectrometer

Supplied Name:

Supplied Description:

To measure dissolved gases

Instrument Type

Generic Name: Membrane Inlet Mass Spectrometer

Acronym: MIMS

Generic Description:

Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.

Pump

Supplied Name: unfiltered seawater pump

Supplied Description:

Used to obtain seawater samples

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
date_plot	sampling date (UTC), formatted to allow sequential plotting of time variable	year-month-day	date
temp	As measured by CTD at 10 m depth	degrees celsius	temp
sal	Salinity	parts per thousand	sal
pCO2	CO2 partial pressure. Calculated from TA; pH; temperature salinity; phosphate and silicate using CO2Sys	ppm	pCO2
CO2_aq	CO2 aqueous. Calculated from TA; pH; temperature; salinity; phosphate and silicate using CO2Sys	micromole/kilogram	no_bcodmo_term
TA_meas	Total alkalinity. Measured in 0.2micron filtered and poisoned seawater by potentiometric titration with average precision of +/- 7 micromole/kg	micromole/kilogram	TALK
TA_sd	Total alkalinity standard deviation	micromole/kilogram	std_dev
DIC_cal	Dissolved Inorganic Carbon. Calculated from TA; pH; temperature; salinity; phosphate and silicate using CO2Sys	micromole/kilogram	DIC
pH	calibrated potentiometrey (until mid-Dec) and spectrophotometrically (rest) and adjusted for temperature	total scale	pH
NO3_NO2_diss_avg	Dissolved nitrate and nitrite	micromolar	NO3_NO2
NO3_NO2_diss_stdev	Dissolved nitrate and nitrite standard deviation	micromolar	std_dev
SiO4_diss_avg	Dissolved silicate	micromolar	SiO4
SiO4_diss_stdev	Dissolved silicate standard deviation	micromolar	std_dev
PO4_diss_avg	Dissolved phosphate	micromolar	PO4
PO4_diss_stdev	Dissolved phosphate standard deviation	micromolar	std_dev
Chl_a_avg	Chlorophyll a concentrations; as measured with turner fluorometer	micrograms/liter	chl_a
Chl_a_stdev	Chlorophyll a standard deviation	micrograms/liter	std_dev
NPP_14C_avg	Net Primary Production as measured by 14C bottle incubations (24 h)	C (micromoles)/liter/day	PP
NPP_14C_stdev	NPP 14C standard deviation	C (micromoles)/liter/day	std_dev
GPP_14C_avg	Gross Primary Production as measured by 14C bottle incubations (2 h)	C (micromoles)/liter/day	PP
GPP_14C_stdev	GPP 14C standard deviation	C (micromoles)/liter/day	std_dev
GPP_18O_avg	Gross Primary Production as measured by production of 18O2 from H218O water (~6h)	O2 micromoles/liter/day	PP
GPP_18O_stdev	GPP 18O standard deviation	O2 micromoles/liter/day	std_dev
NPP_O2_Ar_avg	Net Primary Production as measured by change in O2 with respect to Argon in bottle incubations (~6h)	O2 micromoles/liter/day	PP
NPP_O2_Ar_stdev	NPP O2/Ar standard deviation	O2 micromoles/liter/day	std_dev
POC	Particulate Organic Carbon as measured on Elemental Combustion Analyzer	micromoles/liter	POC
PON	Particulate Nitrogen as measured on Elemental Combustion Analyzer	micromoles/liter	PON
C_N	Carbon to nitrogen ratio of particulate matter; calcuated from POC and PON	mol:mol	C_to_N
POC_d13C	Isotopic ratio of 13C to 12C in POC normalised to PDB standard	per mil	POC
Protein	Total protein from particulate matter as measured by BCA Assay	microgram/liter	no_bcodmo_term
Rubisco	Percent of the carboxylating enzyme Ribulose 1;5 bisphosphate carboxylase oxygenase (Rubisco) as measured by quantitative immunoblotting	% total protein (mg:mg)	no_bcodmo_term
Fuco	pigment concentration of fucoxanthin; as measured by HPLC	micrograms/liter	no_bcodmo_term
HexFuc19	pigment concentration of 19'Hexanoyloxyfucoxanthin; as measured by HPLC	micrograms/liter	no_bcodmo_term
Allox	pigment concentration of alloxanthin; as measured by HPLC	micrograms/liter	allox
diatom	Calculated from pigments using equation 0.9(Fuco-0.0519'HexFuc)	cells/milliliter	diatom
Phaeo	Calculated from pigments using equation 1.25*19'HexFuc	cells/milliliter	phaeo
Cryptophyte	Calculated from pigments using equation 0.7*Allox	cells/milliliter	no_bcodmo_term
eCA	Activity of external carbonic anhydrase (eCA) using the depletion of 18O of aqueous 13C18O2 caused by hydration and dehydration steps of CO2 and bicarbonate. Shown as the ratio of catalyzed CO2/bicarbonate conversion rate compared to the uncatalyzed rate and normalised to chlorophyll. Units are Fold Activity compared to uncatalyzed rate; normalised to chlorophyll concentrations.	milligrams Chl-a/liter (mg/L)	no_bcodmo_term
fHCO3_avg	Proportion of bicarbonate of the total inorganic carbon taken up by the cells to support photosynthesis. Measured using the disquilibrium technique	dimensionless	bicarbonate
fHCO3_stdev	fHCO3 standard deviation	dimensionless	std_dev
K1_2_CO2_avg	Michaelis-Menten half-saturation constant for the CO2 concentration that results in half maximum rate of carbon fixation rate of the natural phytoplankton community concentrated onto a 2micron filter	micromolar	no_bcodmo_term
K1_2_CO2_stdev	K1/2(CO2) standard deviation	micromolar	std_dev
lat	latitude; north is positive	decimal degrees	lat
lon	longitude; east is positive	decimal degrees	lon
year	sampling year	year	year
julian_day	day of year	days	julian_day

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Dataset: Phytoplankton physiology