Dataset: 16S rRNA gene NCBI ID numbers from the PICO project

This dataset has not been validatedFinal no updates expectedVersion Final (2017-06-13)Dataset Type:Other Field Results

Principal Investigator, Contact: Zackary I. Johnson (Duke University)

Co-Principal Investigator: Dana Hunt (Duke University)

BCO-DMO Data Manager: Hannah Ake (Woods Hole Oceanographic Institution)

Program: Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA) (SEES-OA)

Project: Pivers Island Coastal Observatory (PICO)

Project: Collaborative Research: Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments (OA microbe adaptation)

Cite Dataset

Sequences for 16S rRNA gene (bacterioplankton) amplicon libraries for size-fractionated libraries including the large-particle-associated bacterial communities (>63um), small-particle-associated bacterial communities (63 to 5 um), ambiguous bacterial communities (e.g. either large cells or those attached to small particles; 5 to 1-um), and free-living bacterial communities (<1 um).

For related research, go to: http://oceanography.ml.duke.edu/johnson/research/pico/

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Related Publications

Methods, Results
Ward, C. S., Yung, C.-M., Davis, K. M., Blinebry, S. K., Williams, T. C., Johnson, Z. I., & Hunt, D. E. (2017). Annual community patterns are driven by seasonal switching between closely related marine bacteria. The ISME Journal, 11(6), 1412–1422. doi:10.1038/ismej.2017.4
Methods, Results
Yung, C.-M., Ward, C. S., Davis, K. M., Johnson, Z. I., & Hunt, D. E. (2016). Insensitivity of Diverse and Temporally Variable Particle-Associated Microbial Communities to Bulk Seawater Environmental Parameters. Applied and Environmental Microbiology, 82(11), 3431–3437. doi:10.1128/aem.00395-16
Methods
Hugoni, M., Taib, N., Debroas, D., Domaizon, I., Jouan Dufournel, I., Bronner, G., … Galand, P. E. (2013). Structure of the rare archaeal biosphere and seasonal dynamics of active ecotypes in surface coastal waters. Proceedings of the National Academy of Sciences, 110(15), 6004–6009. doi:10.1073/pnas.1216863110
Methods
Kozich, J. J., Westcott, S. L., Baxter, N. T., Highlander, S. K., & Schloss, P. D. (2013). Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform. Applied and Environmental Microbiology, 79(17), 5112–5120. doi:10.1128/aem.01043-13
Methods
Massana R, Murray AE, Preston CM, DeLong EF (1997). Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. Applied and Environmental Microbiology 63: 50-56.

Johnson, Z. I., Hunt, D. (2017) 16S rRNA gene NCBI ID numbers from the PICO project. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2017-06-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/704695 [access date]

Terms of Use

This dataset is licensed under Creative Commons Attribution 4.0.


If you wish to use this dataset, it is highly recommended that you contact the original principal investigators (PI). Should the relevant PI be unavailable, please contact BCO-DMO (info@bco-dmo.org) for additional guidance. For general guidance please see the BCO-DMO Terms of Use document.

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Funded by the U.S. National Science Foundation