Dataset: Trichodesmiium field metaproteomes - sample provenance
Deployment: JC150

Principal Investigator:

Mak A. Saito (Woods Hole Oceanographic Institution, WHOI)

Student:

Noelle Held (Woods Hole Oceanographic Institution, WHOI)

BCO-DMO Data Manager:

Amber D. York (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Collaborative Research: Iron and phosphorus balanced limitation of nitrogen fixation in the oligotrophic ocean (TriCoLim)

Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions (Cyanobacteria Warming Responses)

Marine Microbial Investigator Award: Investigator Mak Saito (MM Saito)

New technology for high resolution analysis of proteins and other organic materials produced by marine microorganisms (MM Proteins and Organics Tech)

Deployment Synonyms:

Ziploc

Data URL:

https://www.bco-dmo.org/dataset-deployment/787139/data

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Description

Trichodesmium sample provenance collected in North Atlantic surface waters, at station BATS (Bermuda Atlantic Time-series Study), and station ALOHA (A Long-Term Oligotrophic Habitat Assessment) between 2000 and 2018. These results were published in Held et al. (2020).

Related Datasets:
Trichodesmium Metaproteomes: https://www.bco-dmo.org/dataset/787078
Trichodesmium single colonies metaproteomes: https://www.bco-dmo.org/dataset/786694
Trichodesmium field metaproteomes - peptide spectral counts: https://www.bco-dmo.org/dataset/787168
Trichodesmium field metaproteomes - protein spectral counts: https://www.bco-dmo.org/dataset/787147
Trichodesmium field metaproteomes - sequence fasta: https://www.bco-dmo.org/dataset/787181

Methods & Sampling

Dataset acquisition description

A 200mm plankton net was deployed to ~20m depth, then recovered to just below surface, repeating five times. Trichodesmium colonies were hand-picked into 0.2mm filtered surface seawater, rinsed twice in 0.2mm filtered surface seawater, and decanted onto a 0.2-4mm Supor filter.

Data Processing Description

Dataset Processing Description

BCO-DMO Data Manager Processing Notes:

* added a conventional header with dataset name, PI name, version date

* modified parameter names to conform with BCO-DMO naming conventions (spaces, +, and - changed to underscores). Units in parentheses removed and added to Parameter Description metadata section.

* blank values in this dataset are displayed as "nd" for "no data." nd is the default missing data identifier in the BCO-DMO system.

* micron unit characters "uM" removed from filter size values in the data table and added to the units in parameter description section.

* Date format changed to ISO 8601 format yyyy-mm-dd
* As discussed with PI, if latitude and longitude are integers, use one decimal place of precision. So .0 added to integer lat/lons. Locations were intentionally selected to fall on integer degrees in some cases.

More information about this dataset deployment

Funding

Award Number	Funding Source
GBMF3782	Gordon and Betty Moore Foundation: Marine Microbiology Initiative
GBMF3934	Gordon and Betty Moore Foundation: Marine Microbiology Initiative
OCE-1657766	NSF Division of Ocean Sciences
OCE-1850719	NSF Division of Ocean Sciences

Instruments

None available yet

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
Name	Sample name	unitless	sample
Replication	Replication (singlicate\|duplicate\|triplicate)	unitless	sample_descrip
Cruise	Cruise identifier (or station BATS or HOT)	unitless	cruise_id
Cruise_station_num	Station number	unitless	station
Time_sampled	Description of time sampled	unitless	sample_descrip
Date_sampled	Date of sample	unitless	date
Net_size	Plankton net mesh size	microns (um)	net_mesh
Filter_size	Supor filter size	microns (um)	filter_size
Latitude	Latitude	decimal degrees	lat
Longitude	Longitude	decimal degrees	lon
time_zone	Local time zone used for columns Date_sampled and Time_sampled.	unitless	time_zone

Database

Contribute Data

Dataset: Trichodesmiium field metaproteomes - sample provenance
Deployment: JC150

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: Trichodesmiium field metaproteomes - sample provenanceDeployment: JC150

Dataset acquisition description

Dataset Processing Description

Dataset: Trichodesmiium field metaproteomes - sample provenance
Deployment: JC150