Dataset: Nematostella ocean acidification experiment: Male Fecundity

Name: Male fecundity following parental ocean acidification during lab experiments conducted in spring 2022.
Published: 2024-03-18
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.920909.1

Laboratory at the University of Pennsylvania

Temporal Extent: 2022-01-26 - 2022-05-02

Project:

Influence of environmental pH variability and thermal sensitivity on the resilience of reef-building corals to acidification stress (Coral Resilience)

Principal Investigator:

Katie Barott (University of Pennsylvania, Penn)

Scientist:

Kristen Brown (University of Pennsylvania, Penn)

Kelsey Speer (University of Pennsylvania, Penn)

Student:

Benjamin Glass (University of Pennsylvania, Penn)

Angela Schmitt (University of Pennsylvania, Penn)

Contact:

Benjamin Glass (University of Pennsylvania, Penn)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2024-03-18

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Male fecundity following parental ocean acidification during lab experiments conducted in spring 2022.

Abstract:

Ocean acidification (OA) resulting from anthropogenic CO2 emissions is impairing the reproduction of marine organisms. While parental exposure to OA can protect offspring via carryover effects, this phenomenon is poorly understood in many marine invertebrate taxa. We examined how parental exposure to acidified (pH 7.40) versus ambient (pH 7.72) seawater influenced reproduction and offspring performance across six gametogenic cycles (13 weeks) in the estuarine sea anemone Nematostella vectensis. This dataset pertains to the reproductive physiology of adult organisms from this study: male fecundity.

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Archival Copy

Version Date	Archive	Persistent Identifier (PID)	Date PID Issued
2024-03-18	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.920909.1	2024-03-24

Description

Data generated as part of a Nematostella ocean acidfication experiment published in Glass et al., 2023. (see Related Publications). Related Zenodo datasets provides further analysis and plotting of the BCO-DMO dataset here. (see Related Dataset).

Methods & Sampling

Nematostella vectensis (Stephenson, 1935) anemones were collected from a salt marsh in Brigantine, New Jersey in the fall of 2020. Females were identified by inducing spawning, and 14 individuals that released eggs were chosen as the genotype pool for this experiment. Each female was then horizontally bisected through the body column using a razor blade, resulting in two genotypically identical individuals that were divided between the two experimental groups (ambient and acidic).

A clonal male population, also originating from the United States Atlantic coast, was obtained from the laboratory of Dr. Katerina Ragkousi (Amherst College) in the spring of 2021. The male population size was increased via bisection, resulting in a total of 20 genetically identical males for the experiment (N=10 per treatment).

All anemones were kept in 12 parts per thousand (ppt) artificial seawater (ASW; Instant Ocean Reef Crystals^Ⓡ reef salt, Spectrum Brands, Blacksburg, VA, USA) at pH 7.7–8.1 and 18°C. The animals were maintained in a dark incubator (Boekel Scientific, Feasterville-Trevose, PA, USA) and fed approximately every other day with Artemia nauplii. The experiment was performed approximately 1–1.5 years after animal collection.

Data Processing Description

After the males spawned, the ASW containing live sperm (hereafter referred to as ‘sperm water’) was filtered through a 100 μm cell strainer (Corning, Corning, New York, USA) into a 50 ml conical tube to remove debris. Sperm concentrations were quantified using a hemocytometer (Marienfeld, Lauda-Königshofen, Germany) in weeks 2 and 4. Specifically, 1 ml of sperm water from each conical was transferred to a 1.5 ml tube and centrifuged once at 1500×g for 5 min at 22°C; then, the supernatants removed, and the sperm pellets resuspended in 110 μl of 12 ppt ASW. Next, 10 μl aliquots of the concentrated sperm from each treatment were loaded separately onto a hemocytometer, and cells were counted under 10× magnification according to the manufacturer's instructions. Sperm concentrations were divided by the number of males in each container to determine the average number of sperm produced per male. For weeks 7–13, sperm concentration was measured for each individual male anemone with a Guava^Ⓡ easyCyte™ HT flow cytometer (MilleporeSigma, St. Louis, MO, USA) in triplicate (as technical replicates) in accordance with the manufacturer's instructions.

Data Files

File
920909_v1_malefecundity.csv (Comma Separated Values (.csv), 1.29 KB) MD5:8801b8aec2f0e563f0b876c9d7f35af4 Primary data file for dataset ID 920909, version 1

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1923743

Deployments

None available yet

Instruments

Flow Cytometer

Supplied Name: GuavaⓇ easyCyte™ HT flow cytometer (MilleporeSigma, St. Louis, MO, USA)

Supplied Description:

GuavaⓇ easyCyte™ HT flow cytometer (MilleporeSigma, St. Louis, MO, USA) for sperm concentration determination

Instrument Type

Generic Name: Flow Cytometer

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Generic Description:

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Treatment	Experimental treatment into which anemones were placed (ambient or acidic seawater pH)	unitless	treatment
Date	Sampling week (week 1 start = 2022-01-26)	unitless	time_point
Sperm_Released	Number of sperm released by a single male	unitless	number