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Experimental conditions applied to experimental units. In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.
Experimental conditions varied in order to observe a variable's effect. Four treatments were used: (1) 12 deg C and 390 ppm CO2 (LTLC), (2) 12 deg C and 690 ppm CO2 (LTHC), (3) 16 deg C and 390 ppm CO2 (HTLC), and (4) 16 deg C and 690 ppm CO2 (HTHC).