This dataset is from CTD-based water collections of samples for phytoplankton and non-pigmented bacteria in the Gulf of Mexico on R/V Nancy Foster cruises in May 2017 and May 2018, which were part of a NOAA RESTORE project (aka: BLOOFINZ-GoM) led by Dr. John Lamkin to investigate the epipelagic marine nitrogen cycle, plankton dynamics, and impacts on growth and survival of larval Atlantic Bluefin Tuna (ABT). These data are meant to be used in inter-species, interregional comparisons to data from the BLOOFIN-IO study of larval larval Southern Bluefin Tuna in the Indian Ocean spawning region. Flow cytometry results include abundances of phytoplankton taxa (Prochlorococcus, Synechococcus, Photosynthetic Eukaryotes) and non-pigmented bacteria (HBACT).
Flow cytometry samples were collected at sea from each CTD rosette on the NOAA Ship Nancy Foster (NF) from cruises from 30 April to 19 May 2017 and from 11 May to 30 May 2018. For phytoplankton and heterotrophic bacteria enumeration, 2 ml sample volumes are collected, preserved (0.5% paraformaldehyde, final concentration) and flash frozen in liquid nitrogen. On shore, the samples are transferred to a –80°C freezer for storage until analysis on the flow cytometer. Samples are thawed in batches, then stained with Hoechst 34442 (1 µg/ml, final concentration) (Monger and Landry, 1993; Campbell and Vaulot, 1993; Campbell et al., 1994).