Name: Eelgrass shoot density measurements taken during ecological field surveys along the eastern Pacific coast in June through August of 2019, 2020, and 2021.
Published: 2022-10-13
Keywords: seagrass wasting disease, Zostera marina, intertidal, eelgrass meadow, biota, oceans

Data File(s)	Type	Description	Action
shoot_density.csv (299.11 KB)	Comma Separated Values (.csv)	Primary data file for dataset ID 879764	Download View Table

Supplemental File(s)	Type	Description	Action
eelgrass_study_revised_site_metadata.csv (50.38 KB)	Comma Separated Values (.csv)	Combined site metadata for eelgrass wasting disease study sites on the western coast of the U.S. and Canada SampleCollectionDate: Date when samples were collected in the field Region: Two-letter identifier for the geographic region where the sample was collected (AK=Alaska, BC=British Columbia, WA=Washington, OR=Oregon, BB=Bodega Bay in California, SD=San Diego in California) SiteCode: One-letter identifier for site within a geographic region where the sample was collected (A, B, C, D, E, F) LocationName: Full name of each sampling site (eelgrass meadow) where samples were taken TidalHeight: Single letter indicating the tidal height at which samples were collected. U = upper tidal height; L = lower tidal height Transect: Integer indicating the transect at which samples were collected. Upper transects = 1, 2, 3; Lower transects = 4, 5, 6. SampleProcessingDate: Date when samples were processed in the lab Depth: Depth relative to MLLW (mean lower low water) Salinity: Salinity of surface water at the time of sampling (point measurement made with refractometer or probe) LocationComments: Comments describing location including changes between sampling year TransectBeginDecimalLatitude: Latitudinal coordinate for the beginning (meter 0) of the transect TransectBeginDecimalLongitude: Longitudinal coordinate for the beginning (meter 0) of the transect TransectEndDecimalLatitude: Latitudinal coordinate for the end (meter 20) of the transect TransectEndDecimalLongitude: Longitudinal coordinate for the end (meter 20) of the transect Year: Year in which samples were collected	Download
eelgrass_disease_project_handbook_v1.2.pdf (1.38 MB)	Portable Document Format (.pdf)	NSF Eelgrass Disease Project 2019 Handbook v1.2 from MarineGEO	Download

Files

Filename: shoot_density.csv (299.11 KB)
Type: Comma Separated Values (.csv)
Description: Primary data file for dataset ID 879764

Download View Table

Supplemental Files

Filename: eelgrass_study_revised_site_metadata.csv (50.38 KB)
Type: Comma Separated Values (.csv)
Description: Combined site metadata for eelgrass wasting disease study sites on the western coast of the U.S. and Canada SampleCollectionDate: Date when samples were collected in the field Region: Two-letter identifier for the geographic region where the sample was collected (AK=Alaska, BC=British Columbia, WA=Washington, OR=Oregon, BB=Bodega Bay in California, SD=San Diego in California) SiteCode: One-letter identifier for site within a geographic region where the sample was collected (A, B, C, D, E, F) LocationName: Full name of each sampling site (eelgrass meadow) where samples were taken TidalHeight: Single letter indicating the tidal height at which samples were collected. U = upper tidal height; L = lower tidal height Transect: Integer indicating the transect at which samples were collected. Upper transects = 1, 2, 3; Lower transects = 4, 5, 6. SampleProcessingDate: Date when samples were processed in the lab Depth: Depth relative to MLLW (mean lower low water) Salinity: Salinity of surface water at the time of sampling (point measurement made with refractometer or probe) LocationComments: Comments describing location including changes between sampling year TransectBeginDecimalLatitude: Latitudinal coordinate for the beginning (meter 0) of the transect TransectBeginDecimalLongitude: Longitudinal coordinate for the beginning (meter 0) of the transect TransectEndDecimalLatitude: Latitudinal coordinate for the end (meter 20) of the transect TransectEndDecimalLongitude: Longitudinal coordinate for the end (meter 20) of the transect Year: Year in which samples were collected

Download

Filename: eelgrass_disease_project_handbook_v1.2.pdf (1.38 MB)
Type: Portable Document Format (.pdf)
Description: NSF Eelgrass Disease Project 2019 Handbook v1.2 from MarineGEO

Download

Field surveys of eelgrass meadow sites were conducted at mid-summer low tides at field sites along the west coast of North America in the U.S. and Canada. Samples and data were collected within the intertidal area of 32 eelgrass meadows distributed in six regions (Alaska, British Columbia, Washington, Oregon, California -Bodega Bay, and California -San Diego). Surveys were conducted between late June and early August in 2019, 2020, and 2021 by teams from six institutions.

For each site, three 20 meter transects were laid parallel to the shore at the shoreward (upper edge) of continuous eelgrass, and three lower (intertidal) 20 meter transects were laid at least 4 meters closer to the water. Shoot density and canopy cover were measured at meters 4, 8, 12, and 16. At each meter, a PVC quadrat was placed on the upper (landward) side of the transect tape, aligning the lower left corner of the quadrat with the meter mark. The percent cover within the quadrat area was recorded for seagrass, bare sediment, and other (macroalgae unless noted otherwise). Shoot densities, categorized by the number of vegetative and flowering shoots, were then counted. Only shoots rooted in the quadrat were included in the density counts. Quadrats for shoot density range in size from 0.0625 to 0.36 square meters. Quadrats for cover ranged in size from 0.09 to 1 square meter. Quadrat size was determined by expert judgment of the practitioners at each field site.

Transect locations were recorded using a hand-held GPS (exact model varied between field locations). Salinity was measured at the time of sampling using a refractometer. Temperature loggers (HOBO MX 2201 and UA-001-64, Onset, Bourne, MA) were deployed at each eelgrass meadow site to provide a continuous record of in situ temperature. For HOBO data, see https://www.bco-dmo.org/dataset/877355 and Related Datasets section below.

~ For methodology details, see Aoki et al. (2022)
~ Additional details for the field surveys are available in the Eelgrass Disease Project Handbook.
~ For 16S rRNA amplicon sequencing of eelgrass associated bacteria, refer to NCBI BioProject PRJNA802566 in the Related Datasets section below.

Related Datasets

IsRelatedTo

Dataset: Eelgrass Disease Metrics

Harvell, D., Gomes, C. P., Hawthorne, T., Stachowicz, J. J., Duffy, J. E., Aoki, L. (2022) Eelgrass disease metrics from ecological field surveys along the eastern Pacific coast in June through August of 2019, 2020, and 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-13 doi:10.26008/1912/bco-dmo.879780.1

IsRelatedTo

Dataset: Eelgrass Shoot Metrics

Harvell, D., Gomes, C. P., Hawthorne, T., Stachowicz, J. J., Duffy, J. E., Aoki, L. (2022) Eelgrass shoot metrics from ecological field surveys in six regions along the eastern Pacific coast in June through August of 2019, 2020, and 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-13 doi:10.26008/1912/bco-dmo.878857.1

IsSupplementedBy

Dataset: HOBO temperatures from eelgrass field surveys

Harvell, D., Gomes, C. P., Hawthorne, T., Stachowicz, J. J., Duffy, J. E., Aoki, L. (2022) In situ temperature measurements from eelgrass meadow field sites along the west coast of North America recorded from July 2019 to July 2021. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2022-10-14 doi:10.26008/1912/bco-dmo.877355.1

IsSupplementedBy

Dataset: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA802566

University of California, Davis. 16S rRNA amplicon sequencing of eelgrass associated bacteria. 2022/02. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA802566. NCBI:BioProject: PRJNA802566.

Related Publications

Methods

Rappazzo, B. H., Eisenlord, M. E., Graham, O. J., Aoki, L. R., Dawkins, P. D., Harvell, D., & Gomes, C. (2021). EeLISA: Combating Global Warming Through the Rapid Analysis of Eelgrass Wasting Disease. Proceedings of the AAAI Conference on Artificial Intelligence, 35(17), 15156-15165. Retrieved from https://ojs.aaai.org/index.php/AAAI/article/view/17779

Related Research

Aoki, L. R., Rappazzo, B., Beatty, D. S., Domke, L. K., Eckert, G. L., Eisenlord, M. E., Graham, O. J., Harper, L., Hawthorne, T. L., Hessing‐Lewis, M., Hovel, K. A., Monteith, Z. L., Mueller, R. S., Olson, A. M., Prentice, C., Stachowicz, J. J., Tomas, F., Yang, B., Duffy, J. E., … Harvell, C. D. (2022). Disease surveillance by artificial intelligence links eelgrass wasting disease to ocean warming across latitudes. Limnology and Oceanography, 67(7), 1577–1589. Portico. https://doi.org/10.1002/lno.12152

Dataset: Eelgrass shoot density measurements taken during ecological field surveys along the eastern Pacific coast in June through August of 2019, 2020, and 2021.

Principal Investigator: Drew Harvell (Cornell University)

Co-Principal Investigator: J. Emmett Duffy (Smithsonian Environmental Research Center)

Co-Principal Investigator: Carla P. Gomes (Cornell University)

Co-Principal Investigator: Timothy Hawthorne (University of Central Florida)

Co-Principal Investigator: John J. Stachowicz (University of California-Davis)

Scientist, Data Manager: Lillian Aoki (Cornell University)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

Project: Collaborative Research: The role of a keystone pathogen in the geographic and local-scale ecology of eelgrass decline in the eastern Pacific (Eelgrass disease)

Abstract

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Supplemental Files

Related Datasets

Related Publications

Dataset: Eelgrass shoot density measurements taken during ecological field surveys along the eastern Pacific coast in June through August of 2019, 2020, and 2021.

Principal Investigator: Drew Harvell (Cornell University)

Co-Principal Investigator: J. Emmett Duffy (Smithsonian Environmental Research Center)

Co-Principal Investigator: Carla P. Gomes (Cornell University)

Co-Principal Investigator: Timothy Hawthorne (University of Central Florida)

Co-Principal Investigator: John J. Stachowicz (University of California-Davis)

Scientist, Data Manager: Lillian Aoki (Cornell University)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)

Project: Collaborative Research: The role of a keystone pathogen in the geographic and local-scale ecology of eelgrass decline in the eastern Pacific (Eelgrass disease)

Abstract

Metadata

Files

Supplemental Files

Related Datasets

Related Publications