Dataset: Thermal Stress Experiment: Experimental Tank Conditions

Name: Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.
Published: 2023-01-31
Keywords: oceans

Cite This Dataset

DOI:10.26008/1912/bco-dmo.886196.1

Project:

NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration (Holobiont Integration)

Co-Principal Investigator:

Hollie Putnam (University of Rhode Island, URI)

Student:

Emma Strand (University of Rhode Island, URI)

BCO-DMO Data Manager:

Karen Soenen (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Version:

Version Date:

2023-01-31

Restricted:

Validated:

Yes

Current State:

Final no updates expected

Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.

Abstract:

Experimental tank conditions through the four day acclimation period and five week thermal stress experiment. Temperature in each tank was logged every 10 minutes as well as discrete measurements of temperature, pH, salinity, and conductivity made twice daily. Flow rates of each tank were measured once a day, and light (PAR) was measured once daily in the morning.

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Archival Copy

Version Date	Archive	Persistent Identifier	Date Assigned
2023-01-31	Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)	10.26008/1912/bco-dmo.886196.1	2023-01-31

Methods & Sampling

Cultivation of coral nubbins:From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species M. capitata and P. acuta were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. The 30 glued nubbins of each genotype were then randomly distributed among six tanks (~32 liters; 48.3 cm by 38.1 cm by 17.8 cm; L × W × H), leaving five replicates per genotype in each tank for a total of 40 coral nubbins per tank and 120 coral nubbins per species. Tanks were placed in a flow-through system that had a steady supply of water directly from Kāne‘ohe Bay with an average flow rate of (173.8 ± 73 liters hour−1, mean ± SD). Each tank was fitted with a submersible pump (Hydor 200 gph), a HOBO Water Temp Pro temperature logger (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.), an Apex temperature probe (Neptune Systems), and two heaters (Aqueon 300-W Heater set to 31°C and DaToo 300-W Glass Heater set to 34°C). The temperature in the tanks was controlled by powering off and on the heaters based on set points in the Apex aquarium controller (Neptune Systems). Light was set for a 12-hour light/12-hour dark cycle using Arctic T247 lights (Ocean Revive).

Data Files

File
thermal_experiment_conditions.csv (Comma Separated Values (.csv), 5.89 MB) MD5:278f1079369e0b4fc82d465b9ca97496 Primary data file for dataset ID 886196

More Information about this dataset

Funding Sources

Funding Source	Award Number
NSF Division of Ocean Sciences	OCE-1756623

Deployments

None available yet

Instruments

Biospherical PAR sensor

Supplied Name: MQ-510 Full Spectrum Underwater Quantum Meter, Apogee

Supplied Description:

underwater cosine light sensor and meter (MQ-510 Full Spectrum Underwater Quantum Meter, Apogee)

Instrument Type

Generic Name: Biospherical PAR sensor

Generic Description:

Unspecified Biospherical PAR. An irradiance sensor, designed to measure Photosynthetically Active Radiation (PAR).

digital thermometer

Supplied Name:

Supplied Description:

handheld digital certified thermometer (Control Company accuracy, ± 0.05°C; resolution, 0.001°C)

Instrument Type

Generic Name: digital thermometer

Generic Description:

An instrument that measures temperature digitally.

Multi Parameter Portable Meter

Supplied Name: MQ-510 Full Spectrum Underwater Quantum Meter, Apogee

Supplied Description:

Instrument Type

Generic Name: Multi Parameter Portable Meter

Generic Description:

An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and temperature with one device and is portable or hand-held.

Onset HOBO Pro v2 temperature logger

Supplied Name:

Supplied Description:

HOBO Water Temp Pro temperature loggers (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.)

Instrument Type

Generic Name: Onset HOBO Pro v2 temperature logger

Acronym: HOBO U22-001

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

The HOBO Water Temp Pro v2 temperature logger, manufactured by Onset Computer Corporation, has 12-bit resolution and a precision sensor for ±0.2°C accuracy over a wide temperature range. It is designed for extended deployment in fresh or salt water.

Operation range: -40° to 70°C (-40° to 158°F) in air; maximum sustained temperature of 50°C (122°F) in water
Accuracy: 0.2°C over 0° to 50°C (0.36°F over 32° to 122°F)
Resolution: 0.02°C at 25°C (0.04°F at 77°F)
Response time: (90%) 5 minutes in water; 12 minutes in air moving 2 m/sec (typical)
Stability (drift): 0.1°C (0.18°F) per year
Real-time clock: ± 1 minute per month 0° to 50°C (32° to 122°F)

Additional information (http://www.onsetcomp.com/)
Onset Computer Corporation
470 MacArthur Blvd
Bourne, MA 02532

Pump

Supplied Name: submersible pump (Hydor 200 gph)

Supplied Description:

Instrument Type

Generic Name: Pump

Generic Description:

A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps

Water Temperature Sensor

Supplied Name: Apex temperature probe (Neptune Systems)

Supplied Description:

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Date and time formats are described in python strftime() syntax.

Supplied Name	Supplied description	Supplied Units	Standard Name
Date	Date of measurement Format: %Y-%m-%dT%H:%M:%S	unitless	date
PPFD_Time	Time of light measurement Format: %H:%M	unitless	time
Tank	Tank ID	unitless	tank
Position	Position in the tank of light measurement	unitless	sample_descrip
PPFD	PAR light value measured	nm (nanometers)	PAR
Avg_flowrate	Average flow rate measured (n=2)	mL/seconds	flow_rate
HOBO_Time	Time of temperature measurement from HOBO logger Format: %H:%M:%S	unitless	time
HOBO_Temperature	Temperature measurement from HOBO logger	degrees Celsius (°C)	temp
Calibration_date	Date of calibration to Tris measurement Format: %Y%m%d	unitless	date
Discrete_measurement_time	Time of the discrete measurement (Temperature, pH.MV, Salinity, Conductivity) Format: %H:%M	unitless	time
Treatment	High or ambient temperature treatment	unitless	treatment
Temperature	Temperature	degrees Celsius (°C)	temp
pH_MV	pH value	millivolts	pH
Salinity	Salinity	parts per thousand (ppt)	sal
Conductivity	Conductivity	millisemens	cond

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Database

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Dataset: Thermal Stress Experiment: Experimental Tank Conditions