Dataset: Experiment Tank Conditions during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.886196.1Version 1 (2023-01-31)Dataset Type:experimental

Co-Principal Investigator: Hollie Putnam (University of Rhode Island)

Student: Emma Strand (University of Rhode Island)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Project: NSFOCE-BSF: COLLABORATIVE RESEARCH: Elucidating adaptive potential through coral holobiont functional integration (Holobiont Integration)


Abstract

Experimental tank conditions through the four day acclimation period and five week thermal stress experiment. Temperature in each tank was logged every 10 minutes as well as discrete measurements of temperature, pH, salinity, and conductivity made twice daily. Flow rates of each tank were measured once a day, and light (PAR) was measured once daily in the morning.  

Cultivation of coral nubbins:From the waters of Kāne‘ohe Bay, HI, four colonies of each coral species M. capitata and P. acuta were identified and collected under SAP 2019-60. Each of the four colonies for each species was fragmented into 30 pieces at the Hawaiʻi Institute of Marine Biology, located on Moku o Loʻe in Kāne‘ohe Bay, HI, and hot-glued to labeled plugs. The 30 glued nubbins of each genotype were then randomly distributed among six tanks (~32 liters; 48.3 cm by 38.1 cm by 17.8 cm; L × W × H), leaving five replicates per genotype in each tank for a total of 40 coral nubbins per tank and 120 coral nubbins per species. Tanks were placed in a flow-through system that had a steady supply of water directly from Kāne‘ohe Bay with an average flow rate of (173.8 ± 73 liters hour−1, mean ± SD). Each tank was fitted with a submersible pump (Hydor 200 gph), a HOBO Water Temp Pro temperature logger (operation range, −40° to 70°C; resolution, 0.02°C at 25°; accuracy, ±0.21°C from 0° to 50°C; Onset Computer Corp.), an Apex temperature probe (Neptune Systems), and two heaters (Aqueon 300-W Heater set to 31°C and DaToo 300-W Glass Heater set to 34°C). The temperature in the tanks was controlled by powering off and on the heaters based on set points in the Apex aquarium controller (Neptune Systems). Light was set for a 12-hour light/12-hour dark cycle using Arctic T247 lights (Ocean Revive).

 


Related Datasets

IsRelatedTo

Dataset: Thermal Stress Experiment: Targeted Metabolites
Relationship Description: Dataset is part of the same experiment.
Strand, E., Putnam, H. (2023) Metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 doi:10.26008/1912/bco-dmo.886420.1
IsRelatedTo

Dataset: Thermal Stress Experiment: Color Score Physiology
Relationship Description: Dataset is part of the same experiment.
Strand, E., Putnam, H. (2023) Physiology color score extracted from pictures taken during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 doi:10.26008/1912/bco-dmo.884220.1
IsRelatedTo

Dataset: Thermal Stress Experiment: Untargeted Metabolites
Relationship Description: Dataset is part of the same experiment.
Strand, E., Putnam, H. (2023) Untargeted metabolomic data collected during a thermal stress experiment using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2023-01-31 doi:10.26008/1912/bco-dmo.886427.1

Related Publications

No Related Publications