©2020 Biological and Chemical Oceanography Data Management Office.Funded by the U.S. National Science Foundation
An instrument that determines the numbers, types or viability of cells present in a sample.
| Dataset Name | PI-Supplied Description | PI-Supplied Name |
|---|---|---|
| Coccolithophore survival in darkness from batch growth experiments (Cocco-Mix project) | Cell concentration was determined using a hemocytometer on an American Optical Microscope (Spencer Lens Company, Buffalo, NY, USA) with polarization optics for CCMP289, as well as a Moxi Z Cell Counter (Andwin Scientific, Simi Valley, CA, USA) for CCMP3337. The Moxi Z uses Gaussian curve-fitting with a coincidence correction algorithm of cell count (vs. diameter) histograms to extract precise (>95%) cell count metrics in a sample. The extracted raw data was further used for cellular carbon calculations of CCMP 3337. After the experiment, the vials, which were kept in the dark, were placed in the light, and after 10 days we were able to qualitatively confirm, under the microscope, renewed growth of coccolithophore cells. We calculated the carbon content of the cells of CCMP3337 according to the equations for cellular elemental content based on nine isolates covering a wide range of coccolithophore cell diameters and representative of the taxonomic diversity of coccolithophores (Villiot et al., 2021). The basis for these calculations were cell diameters measured by Cell Counter Moxi Z, these were averaged from raw data for each sample that was measured and standard deviation of a frequency distribution was calculated. | Moxi Z Cell Counter (Andwin Scientific, Simi Valley, CA, USA) |
| Physiological parameters of three corals species collected from Hawaii and exposed to four treatment conditions for 22-months as part of a mesocosm experiment | Countess II FL Automated Cell Counter | |
| Size and chemical responses of two dinoflagellate species used in natural high light exposure experiments (Protist Signaling project) | Used to determine cell concentration | Beckman Coulter Z2 Particle Count and Size Analyzer |
| Experimental data on growth rates of Pleurochrysis carterae analyzed at Bigelow Laboratory from 2013. (OA Copes Coccoliths project) | Measures culture density | Moxi Z Automated Cell Counter |
| Prochlorococcus cell concentrations during the BiG-RAPA expedition (Cruise MV1015) in the Peru Current and Eastern South Pacific Subtropical Gyre between November and December of 2010 | Influx Cell Sorter (BD Biosciences and Cytopeia) | |
| Depth profile data from R/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014 | Used to count cells | FACSJazz: Fluorescence Activated Cell Sorter (BD Biosciences) |
| Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project) | measures culture density | Moxi Z Mini Automated Cell Counter |
| Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) | Measures culture density | Moxi Z Automated Cell Counter |
| Pleurochrysis carterae growth cycle culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project) | Measures culture density | Moxi Z Automated Cell Counter |
| Fatty acid profiles by biovolume of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures | CASY particle counter, Schärfe System GmbH, Reutlingen, Germany | |
| Fatty acid profiles per cell of replicate populations of Thalassiosira pseudonana, selected at 16 and 31C for ~500 generations and assayed at 4 temperatures | CASY particle counter, Schärfe System GmbH, Reutlingen, Germany | |
| Transcriptomics of phytoplankton cultures grown on various phosphorus sources in a laboratory experiment | BD FACSJazz cell sorter |
©2020 Biological and Chemical Oceanography Data Management Office.