Dataset: 18S rRNA amplicon sequencing of microbial eukaryotes from the Mid-Cayman Rise acquired Jan-Feb, 2020

ValidatedFinal with updates expectedDOI: 10.26008/1912/bco-dmo.914399.1Version 1 (2023-11-06)Dataset Type:Other Field Results

Principal Investigator, Contact: Sarah K. Hu (Woods Hole Oceanographic Institution)

Principal Investigator: Julie Huber (Woods Hole Oceanographic Institution)

BCO-DMO Data Manager: Karen Soenen (Woods Hole Oceanographic Institution)


Program: Center for Dark Energy Biosphere Investigations (C-DEBI)

Project: Probing subseafloor microbial interactions via hydrothermal vent fluids: A focus on protists (Microbial eukaryotes at hydrothermal vents)


Abstract

Single-celled microbial eukaryotes inhabit deep-sea hydrothermal vent environments and play critical ecological roles in the vent-associated microbial food web. 18S rRNA amplicon sequencing of diffuse venting fluids from two geochemically-distinct hydrothermal vent fields was applied to investigate community diversity patterns among protistan assemblages. Piccard and Von Damm vent fields are situated 20 km apart at the Mid-Cayman Rise in the Caribbean Sea. We describe species diversity patterns ...

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Samples and experiments were collected and executed during cruise AT42-22 aboard the RV Atlantis with ROV Jason in January-February 2020 at the Von Damm (2300 m; 18°23’N, 81°48’W) and Piccard (5000 m; 18°33’N, 81°43’W) hydrothermal fields located along the Mid-Cayman Rise.

Fluids for shipboard grazing experiments and biogeochemistry were obtained in 10L volume bags (Kynar, Keika Ventures; polyvinylidene fluoride) using the Hydrothermal Organic Geochemistry (HOG) sampler mounted on ROV Jason. Between 4-10 L of vent fluid was collected and filtered through a 47 mm polyethersulphone (PES) filter (Millipore) with a pore size of 0.2 µm and preserved with RNAlater (Ambion) at the seafloor for molecular analysis of microbial communities. 

Non-vent samples were collected from within the overlying non-buoyant hydrothermal plume at each site and from background seawater via CTD-mounted Niskin bottles. Plume samples were identified using in situ CTD sensors to detect the presence of hydrothermal influence in real-time (back-scatter and temperature) above each vent field. Background seawater samples were collected outside of the influence of the hydrothermal vent at approximately the same depth as the vent sites (~2350 m and ~4950 m).


Related Datasets

IsRelatedTo

Dataset: Metagenome and metatranscriptome sequences from deep-sea hydrothermal vent microbial communities
Hu, S. K., Anderson, R., Huber, J. (2024) Metagenome and metatranscriptome sequences from deep-sea hydrothermal vent microbial communities collected on cruises AT42-22, TN405, and NA108 from May 2019 to Jun 2022. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2024-12-05 doi:10.26008/1912/bco-dmo.936069.1

Related Publications

Results

Hu, S. K., Anderson, R. E., Pachiadaki, M. G., Edgcomb, V. P., Serres, M. H., Sylva, S. P., German, C. R., Seewald, J. S., Lang, S. Q., & Huber, J. A. (2023). Microbial eukaryotic predation pressure and biomass at deep-sea hydrothermal vents: Implications for deep-sea carbon cycling. https://doi.org/10.1101/2023.08.11.552852
Results

Hu, S. K., Smith, A. R., Anderson, R. E., Sylva, S. P., Setzer, M., Steadmon, M., Frank, K. L., Chan, E. W., Lim, D. S. S., German, C. R., Breier, J. A., Lang, S. Q., Butterfield, D. A., Fortunato, C. S., Seewald, J. S., & Huber, J. A. (2022). Globally‐distributed microbial eukaryotes exhibit endemism at deep‐sea hydrothermal vents. Molecular Ecology. Portico. https://doi.org/10.1111/mec.16745
Related Research

Hu, S. K., Herrera, E. L., Smith, A. R., Pachiadaki, M. G., Edgcomb, V. P., Sylva, S. P., Chan, E. W., Seewald, J. S., German, C. R., & Huber, J. A. (2021). Protistan grazing impacts microbial communities and carbon cycling at deep-sea hydrothermal vents. Proceedings of the National Academy of Sciences, 118(29). https://doi.org/10.1073/pnas.2102674118