Instrument: Liquid Scintillation Counter

Acronym: LSC
External Identifier: skos:broadMatch: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/

Description

Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.

Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample.


Dataset NamePI-Supplied DescriptionPI-Supplied Name
Nutrient concentrations and microbial counts from Niskin bottle collections on R/V Atlantic Explorer A1620 in the Bermuda Atlantic Time-series Study site from Sept 2016Used to count microbial cells
Nutrient concentrations and microbial counts from Niskin bottle collections on R/V Atlantic Explorer A1703 in the Bermuda Atlantic Time-series Study site from Mar/April 2016Used to count microbial cells
Redox data from RV/Atlantic Explorer AE1812 in the northwest Atlantic, May 2018liquid scintillation counter (Perkin Elmer)
Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on April 11, 2018perkinElmer Liquid Scintillation Analyzer
Phosphorus redox and water column phosphorus data from R/V Neil Armstrong AR16 in the Western North Atlantic from 2017-05-04 to 2017-05-20After resting for a few hours, the 33P-radioacitivity in the vials was determined using a liquid scintillation counter (Perkin Elmer).   liquid scintillation counter (Perkin Elmer)
Sediment geochemistry summary from push cores collected during HOV Alvin dives during the R/V Atlantis cruise AT37-06 in the Guaymas Basin from December 2016Beckman Coulter LS 6500 Scintillation Counter
Primary productivity estimates from the incubation of seawater collected at the Bermuda Atlantic Time-series Study (BATS) site from December 1988 through June 2024A Liquid Scintillation Counter is used to calculate the level of radioactivity in the sample and therefore the amount of 14C particulate organic carbon Liquid Scintillation Counter
Bioavailability factor of polyphosphate, nucleotides, and methyl phosphonate in bioassay experiments with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019 Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter.  Packard Tri-Carb liquid scintillation counter
Bioavailability factor of polyphosphate, nucleotides (ATP and AMP) and phosphonate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019Radioactivity was assayed on a Packard Tri-Carb liquid scintillation counter. 
Survey biogeochemical data from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018For microcentrifuge method protocol for determination of bacterial production rates via 3H-Leucine incorporationEnergy window settings: Channel A: 0-19 KeV Channel B: 2-19 KeV Hidex 300 Liquid Scintillation Analyzer
Results from 14C-labeled uptake experiments determining uptake of specific dissolved organic compounds which showed high potential for osmotrophyTri-Carb 3110TR liquid scintillation analyzer (PerkinElmer, Waltham, MA, USA) Tri-Carb 3110TR liquid scintillation analyzer
Net primary productivity (14C) measurements made during quasi‐Lagrangian experiments on R/V Roger Revelle cruise RR2201 in the Argo basin in the Eastern Indian Ocean/Indonesian throughflow during February and March 2022Scintillation counter (Packard tricarb)
Measurements of chemoautotrophy in samples collected on cruise LMG1801 on R/V Laurence M. Gould from January to February 2018Perkin-Elmer LSC
Dark DIC Fixation Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)Disintegrations per minute (DPM) were counted in a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR). Perkin-Elmer Tri-Carb 2910 TR
Heterotrophic Production Rates collected from CliOMZ AT50-10 in the Eastern Pacific Ocean from May to June 2023 (CliOMZ project)Disintegrations per minute (DPM) were counted in a scintillation counter (Perkin-Elmer Tri-Carb 2910 TR). Perkin-Elmer Tri-Carb 2910 TR
Coccolith dissolution experiment for the study: Gut dissolution of coccoliths by Acartia/effect on pellet sinking.Tricarb 3110 TR scintillation counter
Coccolithophore-associated organic biopolymers for fractionating particle-reactive radionuclides (234Th, 233Pa, 210Pb, 210Po, and 7Be)Beckman Model 8100 Liquid Scintillation Counter Beckman Model 8100 Liquid Scintillation Counter
Physiological measurements during Cyanobacteria Crocosphaera iron/warming experimentsWallac System 1400 liquid scintillation counter Wallac System 1400 liquid scintillation counter
Results from experiments examining efficiency of cellular P for CO2- and gross N2-fixation rates by Crocosphaera watsonii (WH0003) as a function of light and pCO2; conducted in the Hutchins Laboratory, USCCO2-fixation rates were determined using a Beckman Coulter LS-6500 Multi-purpose Scintillation Counter. Liquid Scintillation Counter
Results of experiments examining CO2 and gross N2 fixation rates by Crocosphaera watsonii (WH0003) under differing levels of phosphate, light, and pCO2; conducted in the Hutchins Laboratory, USCCO2-fixation rates were determined using a Beckman Coulter LS-6500 Multi-purpose Scintillation Counter. Liquid Scintillation Counter
Results from experiments examining cellular growth, CO2- and N2-fixation by Crocosphaera watsonii (WH0003) as a function of light; conducted in the Hutchins Laboratory, USCA Beckman Coulter LS-6500 Multi-purpose Scintillation Counter was used to determine CO2-fixation rates. Liquid Scintillation Counter
Elemental stoichiometry for Emiliania huxleyi across a range of 12 temperatures from 8.5-28.6CUsed to process radioactive assays. Perkin Elmer (CA) Liquid Scintillation Counter
Intracellular elemental quotas under low and high temperatures for E. huxleyi in constant and fluctuating thermal environmentsUsed to process radioactive assays. Perkin Elmer (CA) Liquid Scintillation Counter
Bacterial protein production on particles obtained by gravity filtration of water collected on RV/Endeavor EN556 (Patterns of activities project)Used to measure incorporated radioactive 3H-leucine LSA scintillation counter, PerkinElmer Tri-Carb 2910TR
Large volume incubation: DOC, bacterial cell concentration and production from RV/Endeavor EN556, June 2015 (Patterns of activities project)Used to measure incorporated radioactive 3H-leucine LSA scintillation counter, PerkinElmer Tri-Carb 2910TR
Measurements of nutrients, bacterial cell concentration and bacterial production from RV/Endeavor EN556, June 2015 (Patterns of activities project)Used to measure incorporated radioactive 3H-leucine LSA scintillation counter, PerkinElmer Tri-Carb 2910TR
DOC uptake rates by coccolithophores and scintillation counts from field experiments in the North Atlantic during R/V Endeavor cruise EN616 in July 2018The radioactivity of the filters was measured using a Tri-Carb 3110TR time-resolved liquid scintillation counter (PerkinElmer), set for transformed spectral index of external standards. Tri-Carb 3110TR time-resolved liquid scintillation counter
Bacterial production of bulk water and large volume samples taken aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic.PerkinElmer Tri-Carb 2910TR LSA, PerkinElmer Tri-Carb 3110TR LSA
Rates of primary and bacterial production, and chlorophyll concentrations measured experimentally under ambient and elevated pCO2 (750 or 1100 µatm) from Hawaii Ocean Time-series near Station ALOHA from 2010-2011.Perkin Elmer 2600 liquid scintillation counter
Fall 2011 - Porewater SamplesPorewater samples collected for: Anaerobic Methane Oxidation Analytical Method: Sediment incubations with 14CH4 Instrument: Scintillation Counter Method Reference: Orcutt, B., et al. 2005.  Molecular biogeochemistry of sulfate reduction, methanogenesis and the anaerobic oxidation of methane at Gulf of Mexico methane seeps.  GCA Vol. 69, No. 17, pp. 4267–4281 Scintillation Counter
Fall 2011 - Water Column SamplesPorewater samples collected for: Anaerobic Methane Oxidation Analytical Method: Sediment incubations with 14CH4 Instrument: Scintillation Counter Method Reference: Orcutt, B., et al. 2005.  Molecular biogeochemistry of sulfate reduction, methanogenesis and the anaerobic oxidation of methane at Gulf of Mexico methane seeps.  GCA Vol. 69, No. 17, pp. 4267–4281 Scintillation Counter
Summary geochemistry and radiotracer rates for 30 marine sediment cores covering eight sites and four geochemical regimesBeckman-Coulter LS6500 Liquid Scintillation Counter, calibrated with unquenched 3H and 14C standards from Perkin-Elmer (pn:6008500) Beckman-Coulter LS6500
Metagenomic, metatranscriptomics and 16S rRNA gene sequence data from diel sampling at Groves Creek Marsh, Skidaway Island, GA during July 2014Tubes were then placed in to a liquid scintillation counter (Beckman LS-6500) overnight and measured disintegrations per minute (DPM) for live samples were corrected using DPM recorded for killed controls. liquid scintillation counter (Beckman LS-6500)
Primary productivity measurements for the Hawaii Ocean Time-Series (HOT) program from October 1988 to December 2023 at Station ALOHAliquid scintillation counter (Packard model 4640; United Technologies Inc.) liquid scintillation counter
Results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)Used to measure chl a Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)
Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)Used to measure chl a Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)
Rates of primary and bacterial production measured in situ under ambient and elevated pCO2 (750 µatm) from the Hawaiian Ocean Time Series near Station ALOHA from 2010-2011. Perkin Elmer 2600 liquid scintillation counter
Adenosine triphosphate and microbial biomass measurements from the Chesapeake Bay, sampled aboard RV Fay Slover on June 18, 2019perkinElmer Liquid Scintillation Analyzer
Partition coefficient of 234Th, 233Pa, 210Pb, 210Po, and 7BeThe 210Po activity was analyzed by liquid scintillation counting (Beckman Model 8100 Liquid Scintillation Counter). Beckman Model 8100 Liquid Scintillation Counter
Maximum rates of microbially mediated sulfate reduction from three hydrothermal vents along the Juan de Fuca Ridge from R/V Atlantis cruise AT15-67 in the Juan de Fuca Ridge in 2010The radioactivity of the Zn35S and the remaining sulfate from the supernatant (35SO42-) were measured via liquid scintillation counter in Ultima Gold scintillation cocktail (ThermoFisher Inc., Waltham, MA). liquid scintillation counter
Sediment geochemical and microbial activity data collected on R/V Oden along the East Siberian Arctic Shelf from 2014 (ESAS Water Column Methane project)Used to determine activity of tracer substrate Liquid scintillation counter
Q incubation data from the R/V Melville (MV1405) cruise along the California coast during July 2014Scintillation vials with 0.5 mL of 6M HCl, permitted to degas for 24h were counted. Beckman Coulter LS 6500 scintillation counter
Time-series Niskin-bottle sample data from R/V Hermano Gines cruises in the Cariaco Basin from 1995 through 2017 (CARIACO Ocean Time-Series Program)These vials were refrigerated until they were ready for analysis on a BetaScout (PerkinElmer) scintillation counter. BetaScout (PerkinElmer) scintillation counter
Dissolved inorganic carbon (DIC) fixation and dissolved organic carbon (DOC) release measurements of marine nitrifier cultures grown under different culture conditionsBeckman Coulter LS6500 Scintillation counter
Nitrogen and carbon fixation rates and POC and PON from thermal variation experiment of Trichodesmium GBR strain from 2016-2018Used to measure C14 radioactivity for carbon fixation rates. Perkin Elmer liquid scintillation counter
Standing stocks and rates for organic sulfur compounds during summer in the subarctic Northeast PacificBeckman LS6500 Liquid Scintillation Counter
Cluster (combined temperature, nutrient concentration, and CO2) results from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022Wallac System 1400 liquid scintillation counter
CO2 experiment physiology and carbonate chemistry from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022Wallac System 1400 liquid scintillation counter
Percent activity of organic fractions from diatoms that bind with radionuclideThe 210Po activity was analyzed by liquid scintillation counting (Beckman Model 8100 Liquid Scintillation Counter). Beckman Model 8100 Liquid Scintillation Counter
Percent amount of organic fractions from diatoms that bind with radionuclides The 210Po activity was analyzed by liquid scintillation counting (Beckman Model 8100 Liquid Scintillation Counter). Beckman Model 8100 Liquid Scintillation Counter
Size-fractioned primary production (14C-bicarbonate assimilation rates) from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Packard TRI-Carb 4640
Photosynthetic picoplankton abundances from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Packard TRI-Carb 4640
Measurements of inorganic nutrients (nitrate+nitrite, phosphate, silicate, and ammonium) from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Packard TRI-Carb 4640
Phytoplankton pigments from samples collected during Hawaii Ocean Time-series (HOT) cruises from 2011-2013 (PhytoNsubResponse project)Packard TRI-Carb 4640
Laboratory experiment analyzing the photosynthetic and calcification rates of Pleurochrysis carterae (Ocean acidification effects on copes and coccoliths project)measures radioactivity Packard Tri-Carb 2750 LL
Physiology responses to experimental iron warming interactions of coastal and oceanic Synechococcus collected from the South China SeaBeckman System 6500 liquid scintillation counter
Data on phytoplankton physiology and iron and temperature interactions collected in the Ross Sea during 2013 (Ross Sea Microb Ecophys project)Tri-Carb 2500TR (Packard, now Perkin Elmer
Size fractionated and group-specific rates of 14C-primary production by Prochlorococcus, Synechococcus, and photosynthetic picoeukaryotes from samples collected during Hawaii Ocean Time-series (HOT) program cruises 230-252 between 2012 and 2013Packard TRI-Carb 4640
Pigments and Nutrients from ARSV Laurence M. Gould, RVIB Nathaniel B. Palmer LMG0402, NBP0606 in the Southern Drake Passage and Scotia Sea from 2004-2006 (BWZ project)Liquid Scintillation Counter
Phosphate redox data from cruise AE1409 from R/V Atlantic Explorer AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project)The 33P-radioacitivity in the vials was determined using a liquid scintillation counter (Perkin Elmer).   liquid scintillation counter (Perkin Elmer)
Radioisotope data from B/O Hermano Gines cruises in the CARIACO Basin Time Series Station from May to November 2014 (CariacoMetaOmics project)Activity was measured on this scintillation counter Tri-Carb 2810TR (Perkin Elmer) scintillation counter
Results for radiolabeled acetate, mannitol, and glycerol kinetic uptake and pulse-chase experiments for the species Cruciplacolithus neohelis and Chrysotila carterae (Cocco-Mix project)Following the micro-diffusion step to separate acid-labile (PIC) versus acid-stabile (POC) fractions, each filter was then placed in the bottom of a clean scintillation vial, and scintillation cocktail was added (Balch et al., 2000). The radioactivity was measured using a Tri-Carb 3110TR liquid scintillation analyzer (PerkinElmer, Waltham, MA, USA). Tri-Carb 3110TR liquid scintillation analyzer (PerkinElmer, Waltham, MA, USA)
Compilation of primary productivity measurements collected from 1983-2006 from the Ross SeaVarious LSCs were used, depending on the unit on each ship, but all samples were processed at sea. various
CTD-associated variables, bottle salinity measurements, oxygen titrations, nutrient analyses, biogeochemical/biological variables, and DIC/Freon chemistry variables from R/V Roger Revelle cruise RR2004 along the 150W meridian from 30S to 60SBeckman Tricarb liquid scintillation counter
Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015Used to assay sample radioactivity. Packard Tri-Carb 3110 TR liquid scintillation counter with ultra-low-level option kit
Experiments evaluating protein size distribution pattern in EPS Si+ and EPS Si2 using sodium dodecyl sulfate–polyacrylamide gel electrophoresisThe 210Po activity was analyzed by liquid scintillation counting (Beckman Model 8100 Liquid Scintillation Counter). Beckman Model 8100 Liquid Scintillation Counter
3H-leucine and thymidine incorporation of North Atlantic subseafloor sediments from cruise KN223 on R/V Knorr in the North and West Atlantic Ocean in November 2014Live incubations were terminated with TCA and an extraction protocol modified from Dixon & Turley (2001, Microb. Ecol. 42:549) was used to isolate the protein + DNA fraction, which was analyzed by liquid scintillation counting for 3H-Leu incorporation. liquid scintillation counting
Oceanographic data from 12 monthly cruises off the Israeli coast in the east Mediterranean conducted between Feb 2018 and Jan 2019 at a continental shelf station (~140m depth) and an open-deep water station.TRI-CARB 2100 TR (PACKARD) scintillation counter TRI-CARB 2100 TR (PACKARD) scintillation counter
Environmental data, nutrients, and leucine and thymidine bacterial production from samples collected by CTD during cruises in the San Pedro Channel on R/V Yellowfin from 2005 to 2018Beckman Coulter LS6500
Sulfate reduction rates at Main Endeavor grotto chimney from samples collected on RV Atlantis (AT18-08) during Jason II dives in the Juan de Fuca Ridge from July to August 2011Used to quantify activity Liquid scintillation counter
Continuous culture studies of Synechococcus elongatus CCMP1629 in nitrate-limited and nutrient-replete cultures plus DNA results Used to measure the activity of C-14 in the samples  Packard Tri-Carb model 3100 TR liquid scintillation counter
Continuous culture studies of possible climate change effects: Thalassiosira pseudonana CCMP1335 growth in nitrate-limited and nutrient-replete culturesUsed to measure the activity of C-14 in the samples  Packard Tri-Carb model 3100 TR liquid scintillation counter
Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradianceUsed to measure the activity of C-14 in the samples  Packard Tri-Carb model 3100 TR liquid scintillation counter
Trichodesmium data from R/V Atlantic Explorer cruise AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project)After resting for a few hours, the 33P-radioacitivity in the vials was determined using a liquid scintillation counter (Perkin Elmer). liquid scintillation counter (Perkin Elmer)
Experimental results from a study of CO2 and N2 fixation and trichome length of Trichodesmium erythraeum under varying pCO2 and light conditions (PhytoTM_in_HighCO2 project)Radioactivity of 14C was determined on a Multi-purpose Scintillation Counter (model: LS-6500, Beckman Coulter, Fullerton, CA, USA). Liquid Scintillation Counter
Turnover time and uptake rates of phosphate in treatments amended with dissolved inorganic and organic phosphate compounds in bioassay experiments (incubation 48h) with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring 2019 Radioactivity was assayed on a  Packard Tri-Carb liquid scintillation counter. Packard Tri-Carb liquid scintillation counter
Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019Beckman-Coulter LS6500 liquid scintillation counter
Water column radiotracer methane oxidation rates in the Guaymas Basin, Gulf of California from R/V Atlantis cruise AT42-05 in 2018 and R/V Falkor cruise FK190211 in 2019Perkin Elmer TriCarb liquid scintillation counter
Depth profiles of oceanographic parameters in spring and summer from R/V Terrapin, R/V Hugh R. Sharp multiple cruises in Chesapeake Bay, 2010-2011 (LiDZ project)Radioactivity, expressed as disintegration per minute (DPM), was counted in a liquid scintillation analyzer (Tri-Carb 3100TR, Packard).  The Tri-Carb® 3100TR is a computer-controlled benchtop liquid scintillation analyzer for detecting small amounts of alpha, beta and gamma radioactivity.  (http://shop.perkinelmer.com/Content/relatedmaterials/specificationsheets/spc_tricarb3100trlowactliquidscint.pdf) Liquid Scintillation Analyzer
[Deprecated] Laboratory growth, photosynthetic, and respiration rates of Thalassiosira pseudonana clone 3H in nitrate limited culture (Stressors on Marine Phytoplankton project)Used to measure the activity of C-14 in the samples  Packard Tri-Carb model 3100 TR liquid scintillation counter
[Deprecated] Laboratory growth, photosynthetic, and growth rates of Thalassiosira pseudonana clone 3H in nitrate replete culture (Stressors on Marine Phytoplankton project)Used to measure the activity of C-14 in the samples  Packard Tri-Carb model 3100 TR liquid scintillation counter