Instrument: plate reader

Acronym:
External Identifier: skos:exactMatch: http://vocab.nerc.ac.uk/collection/L05/current/LAB43/

Description

Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.


Dataset NamePI-Supplied DescriptionPI-Supplied Name
Estimated feeding rates of adult and nauplius copepod species on three phytoplankton species from the San Francisco estuary in 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EM filter pair for chlorophyll a.
Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.
Experimental results describing the minimum time for copepod gut fluorescence by plate reader method analyzed at San Francisco State University during 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.
Experimental results describing if sonication is needed for chlorophyll extraction by copepods in the San Francisco estuary conducted during 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.
Experimental results describing Pseudodiaptomus marinus gut minimum fluorescence thresholds that were analyzed at San Francisco State University in 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.
Alkaline phosphatase activities for in situ and incubation samples from RV/Atlantic Explorer cruise AE1812 cruise transect from Bermuda to Rhode Island in May 2018.Samples were run on a Biotek Synergy fluorescent plate reader using black plates Biotek Synergy fluorescent plate reader
Thermal growth for Skeletonema species as analyzed in Anderson and Rynearson, 2020Microplate Reader (Spectramax M Series, Molecular Devices, Sunnyvale, CA)
Alkaline phosphatase activity over bioassay experiments with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019Reading of fluorescence was performed on a plate reader (SpectraMax® M2, Molecular Devices). SpectraMax® M2, Molecular Devices
Alkaline phosphatase activity (APA) Michaelis-Menten kinetic parameters (Vmax and Km) in both whole and filtered seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019Sampling was performed using Niskin bottles (12 L) mounted on a rosette.  Reading of fluorescence was performed on a plate reader (SpectraMax® M2, Molecular Devices). SpectraMax® M2, Molecular Devices
CO2, temperature, and oxygen effects on Atlantic silverside metabolic rates24-channel SensorDish Readers, Presens Precision Sensing, GmbH, Regensburg, Germany. Respirometry oxygen sensor readers
Proximate biochemistry of sponge species collected in 2017 and 2018 across the Caribbean Basin in Curacao, Belize, Grand Cayman, St. CroixBioTek Synergy HT Multi-Detection Microplate Reader
Metabolic potential for heterotrophic utilization of a large array of organics by coccolithophores determined through experiments at Bigelow Laboratory for Ocean Sciences using BioLog Eco-platesFilterMax F5 multimode plate reader (Molecular Devices, LLC, San Jose, CA, U.S.A.). FilterMax F5 multimode plate reader
Brooded coral larval size, protein content, symbiont (Symbiodinium) densities, maximum photochemical efficiency of PSII (Cumbo, 2012) from Taiwan, 2010 (Cumbo, 2012) (MCR LTER project, Climate_Coral_Larvae project)Biotek Synergy H4 Hybrid Reader, USA plate reader
Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)BioRad Life Sciences Research, CA plate reader
Respiration and protein content of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)BioRad Life Sciences Research, CA
Carbonate chemistry data collected as part of a study of the "Community context and pCO2 impact the transcriptome of the "helper" bacterium Alteromonas in co-culture with picocyanobacteria"BioTek H1 plate reader
Chemotaxis of Pseudoalteromonas haloplanktis towards exudates of phage-infected and control Synechoccocus (VIC project)Culture growth was tracked using a SpectraMax ID3 plate reader (Molecular Devices) and cell counts were measured using a CytoFLEX flow cytometer (Beckman Coulter). SpectraMax ID3 plate reader (Molecular Devices)
Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells (VIC project)Culture growth was tracked using a SpectraMax ID3 plate reader (Molecular Devices) and cell counts were measured using a CytoFLEX flow cytometer (Beckman Coulter). SpectraMax ID3 plate reader (Molecular Devices)
Physiology measurements on collected coral pairs from Kaneohe Bay, Oahu, HI between July and December 2019Plate reader used in measuring chlorophyll absorbance BioTek PowerWave XS2
Physiology measurements on collected coral pairs from Kaneohe Bay, Oahu, HI between July and December 2019Plate reader used in measuring protein and antioxidant concentrations BioTek ELx808
Terminal restriction fragments from 16S rRNA genes amplified from coral-associated microbes in response to temperature stress from Kaneohe Bay, Oahu, Hawaii in 2007 (MiCoDe project)SpectraMax M2 plate reader (Molecular Device Corp., Sunnyvale, CA, USA) plate reader
Specificity of associations between bacteria and Pocillopora meandrina during early development in Kaneohe Bay, Oahu, Hawaii from 2008 (MiCoDe project)SpectraMax M2 plate reader (Molecular Device Corp., Sunnyvale, CA, USA) plate reader
T-RFLP, response of the microbial community to coral spawning, lagoon and reef flats in Kaneohe Bay, Oahu, Hawaii during 2006 - 2007 (MiCoDe project)SpectraMax M2 plate reader (Molecular Device Corp., Sunnyvale, CA, USA) plate reader
Dissolved inorganic nutrients from two microcosm incubation experiments conducted on sample water collected from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022used for NOx and NH4 measurements from spring samples Tecan Infinite 200Pro (i-control v2.0.10.0)
Population changes in Halobacteriovorax cultured with protist & preyAbsorbance Microplate Reader Q4 (BIO-TEK Instruments Inc., USA) Absorbance Microplate Reader Q4
Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi (Protist signaling project)Used to measure fluorescent reaction BioTek Synergy M plate reader
Hydrolysis rates from bulk samples, plate reader results from RV/Endeavor EN556, 2015 (Patterns of activities project)TECAN spectrafluor plus
Hydrolysis rates from gravity filtered samples, plate reader results from RV/Endeavor EN556, 2015 (Patterns of activities project)TECAN spectrafluor plus
Measurements of peptidase and glucosidase activities in large volume mesocosm incubations on RV/Endeavor EN584, July 2016 (Patterns of activities project)Used to measure fluorescence of incubated 96-well plate. Settings: 360 nm excitation, 460 emission. TECAN spectrafluor plus
Small substrate hydrolysis of bulk water samples taken aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic.TECAN infiniteF200; 360 nm excitation, 460 emission TECAN infiniteF200
Small substrate hydrolysis of large volume (LV) samples aboard the R/V Endeavor EN638, May 2019 in the Northern Atlantic.TECAN infinite F200; 360 nm excitation, 460 emission TECAN infinite F200
Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016To measure colony counts
Experimental results describing Copepod fed phytoplankton mixtures that were analyzed at San Francisco State University in 2013Tecan Infinite F200 or Biotek Synergy 2 microplate reader was used for each analysis. Each microplate reader contained a 430/20 EX, 680/20 EMfilter pair for Chl a.
Physiological metrics recorded at the end of experiments conducted to examine two coral species' responses to thermal stressSpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices)
Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stressSpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices)
Coral physiology parameters acquired during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.96 well microplate reader (BioTek Synergy HTX Multi-Mode Reader) BioTek Synergy HTX Multi-Mode Reader
High Resolution Growth Screen of Ruegeria pomeroyi Transporter Mutants Data September 2021 - June 2022 (C-CoMP Marine Bacterial Transporters project)Cultures were grown at 25oC in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) shaking at 425 rpm for 68-72 h. OD600 readings were collected once each hour. Synergy H1 plate reader (BioTek, Winooski, VT, USA)
High throughput growth screening of the marine bacterium Ruegeria pomeroyi DSS-3 knockout mutants on 70 environmentally relevant marine substrates done in 2021.SpectraMax M3 plate reader, Molecular Devices, San Jose, CA
Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)Measured baseline absorbance at 412nm. (Fluostar Omega, BMG, Cary, NC, USA) microplate reader
Hydrolytic enzyme activities during CDOM monoculture experiment with CoscinodiscusUsed to measure fluorescence from which hydrolysis rates were calculated. Tecan Infinite 200 Pro microplate reader
Hydrolytic enzyme activities during CDOM monoculture experiments with Skeletonema, Leptocylindrus, and PhaeocystisUsed to measure fluorescence from which hydrolysis rates were calculated. Tecan Infinite 200 Pro microplate reader
Supplementary Table 3B: Replicate cell counts for the 11 samples and alkaline phosphatase activity measurements available for any of the 11 samplesAlkaline Phosphate activity was measured with a Spark 10M Multimode Microplate Reader (Tecan, Männedorf, Switzerland). Spark 10M Multimode Microplate Reader
Restriction site-associated DNA sequence metadata of Kelletia kelletii collected in California, USA and Baja, Mexico in 2015 to 2017Extractions were quantified using the AccuClear Ultra High Sensitivity dsDNA quantification kit (Biotium, Inc) with 3 standards and measured using a SpectraMax M2 microplate reader (Molecular Devices, LLC). SpectraMax M2 microplate reader (Molecular Devices, LLC)
Antibacterial assays of Montipora capitata aqueous extracts collected in Kaneohe Bay, Oahu, Hawaii during 2014BioTek Synergy™ HT Multi-Detection Microplate Reader
Antibacterial assays of Montipora capitata mucus collected in Kaneohe Bay, Oahu, Hawaii during 2014BioTek Synergy™ HT Multi-Detection Microplate Reader plate reader
Pore water geochemistry from sediments collected on cruise OC1703A aboard R/V Oceanus and cruise AT36 aboard R/V AtlantisLab BioTek Synergy HT microplate reader (Winooski, VT)
Coral biometrics data from a heating experiment using samples collected from Nikko Bay and Rebotel Reef in Palau in the spring of 2018Chlorophyll extractions and animal proteins were sampled for absorbance on a Fluostar Omega plate reader (BMG). Fluostar Omega plate reader (BMG)
Physiological response of eight Palau coral colonies to thermal stress as seen in temperature experiments in 2014 and 2015FLUOstar Omega plate reader (BMG Labtech, Germany)
Peptidase and glucosidase activities from mesocosm and bulk water incubations from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022.TECAN infiniteF200; 360 nm excitation, 460 emission
Phosphohydrolysis rates from samples collected in the coastal western North Atlantic on R/V Endeavor cruise EN588 during September 2016multimode plate reader (Molecular Devices)
Photosynthesis and respiration data recorded during experiments conducted to examine two coral species' responses to thermal stressSpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices)
Physiological responses of the coral host and associated endosymbionts measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillataBiotek HT Synergy plate reader
Cell abundance, growth rate, cellular P quotas, and alkaline phosphatase activity from a laboratory experiment examining the response of three species of marine phytoplankton grown under different phosphorus (P) conditionsMolecular Devices FilterMax F5 microplate reader
Polyphosphate and associated measurements from water and particulate samples collected from 13 locations in the Great Lakes in July-Aug 2018 on R/V Blue Heron cruise BH18-09The colorimetric and fluorescence measurements were conducted using a BioTek microplate reader. FlexStation 3 Multi-mode Microplate Reader, BioTek
Stable carbon isotope data for thirteen individual amino acids from twelve species of eukaryotic microalgae and four species of eukaryotic microalgaeMicroplate reader (SpectraMax M5 Series, Molecular Devices) - used to obtain growth rates to target biomass collection approximately during exponential growth or early in the stationary phase. SpectraMax M5 Series microplate reader (Molecular Devices)
Prochlorococcus or Synechococcus cell concentrations and nitrite concentrations during batch culture with ammonium or nitrate as the sole nitrogen source for growthSynergy 2 Plate Reader (BioTek Instruments, Winooski, VT, USA)
Quantification of ciliated band length per unit protein in early echinoderm larvae (protein data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach.Biotek Synergy H1 Hybrid Microplate Reader, to measure spectrophotometric absorption values of protein samples and BSA standards. Biotek Synergy H1 Hybrid Microplate Reader
Ruegeria pomeroyi RB-TnSeq Transponson Mutant Library Screen Experimental Data February 2021 (C-CoMP Marine Bacterial Transporters project)After growth with shaking at 25oC for 72 h in a Synergy H1 plate reader (BioTek, Winooski, VT, USA) , cultures were serially transferred into fresh media four times and then transferred to 1.5 ml tubes, pelleted by centrifugation at 8,000 x g for 3 min, and stored at -80oC until further processing. Synergy H1 plate reader (BioTek, Winooski, VT, USA)
Coral physiology data (chlorophyll a, symbiont densities) on four collected coral species from six sites in the Red Sea from 2018 to 2020SpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices)
RNA:DNA and total lipid measurements for laboratory-based experimental animals collected from the Gulf of Mexico Estuary near Port Aransas, Texas from 2020 to 2022Spectramax 190 Microplate Reader
Dissolved organic phosphorus (DOP) hydrolysis rates from Ruegeria pomeroyi laboratory culturesMolecular Devices M3 multimode plate reader (Spectra Max)
Pore water and solid phase iron geochemical data from a coastal Maine intertidal mudflat from November 2015 to November 2016MultiSkan MCC plate reader
Nutrients, microbiology, trace metals, and environmental conditions from seeded microcosm experimentsTo measure colony counts
Single amplified genomes (SAGs) of chemoautotrophs from global deep sea samples (Dark ocean chemoautotrophs project)high-throughput plate reader (BMG)
Differences in mean oxygen consumption of fed and unfed larvae used to understand the metabolic cost of digestion, Specific Dynamic Action (SDA), under ocean acidification and warming treatments - Experiment 1Oxygen consumption was measured within a closed microplate reader system that uses optical fluorescence to measure oxygen concentration (Loligo Systems, Denmark and PreSens, Germany). closed microplate reader system
Differences in mean oxygen consumption of fed and unfed larvae used to understand the metabolic cost of digestion, Specific Dynamic Action (SDA), under ocean acidification and warming treatments - Experiments 2a and 2bOxygen consumption was measured within a closed microplate reader system that uses optical fluorescence to measure oxygen concentration (Loligo Systems, Denmark and PreSens, Germany). closed microplate reader system
Data on laboratory cultures and statistical analysis code associated with the paper "Co-culture with Synechococcus facilitates the growth of Prochlorococcus under ocean acidification conditions" published in Environmental MicrobiologypH was measured using either a custom-calibrated pH probe or colorimetrically using m-cresol purple administered by sequential injection in a BioTek Synergy H1 spectrophotometer plate reader. BioTek Synergy H1 spectrophotometer plate reader
Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023Molecular Devices M2 multimode plate reader (Spectra Max)
In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023Molecular Devices M2 multimode plate reader (Spectra Max)
Hydrolysis rates from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023Molecular Devices M2 multimode plate reader (Spectra Max)
Fluorescence spectra for 3 strains of Synechococcus while increasing temperatures to detect the photosystem components disassociation temperatureUsed to measure the fluorescence spectra  SpectraMax m2 (Molecular Devices, CA)
Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillataBiotek HT Synergy plate reader
Comparative analysis of tissue biomass and energy reserves of six coral species from nearshore and offshore reefs in Palau, Micronesia during March 2017 All samples were analyzed in triplicate using a microplate reader (EPOCH 2, Agilent), measuring absorbance at 465 nm and 595 nm. microplate reader (EPOCH 2, Agilent)
Daily growth rates for Thermal Performance Curve (TPC) of Chaetoceros simplex after about 100 generations of evolution at seven temperatures, 12-34 degrees C.SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA)
Daily growth rates for Thermal Performance Curve (TPC) of Chaetoceros simplex in nitrogen-replete evolved populations after about 200 generations of evolution at eight temperatures, 10-35 degrees C.SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA)
Water column data from R/V Atlantic Explorer cruise AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project)Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT). Reference: Dyhrman, S.T., Ruttenberg, K.C., 2006. Presence and regulation of alkaline phosphatase activity in eukaryotic phytoplankton from the coastal ocean: Implications for dissolved organic phosphorus remineralization,. Limnol. Oceanogr. 51, 1381-1390. Synergy H1 Hybrid plate reader
Respirometry data for pelagic crustaceans, cephalopods, and fish collected on R/V Sikuliaq cruise SKQ201701S from January to February 2017Presens Plate Reader SY11000, SDR-547: optical oxygen meter with 24 wells (75 µl each) Presens Plate Reader SY11000, SDR-547