| Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments conducted on Hawaii Ocean Time-series (HOT) cruises, HOT283 and HOT288 in 2016 | Cell counts were determined by flow cytometry using a BD Biosciences Influx high speed cell sorter.
| BD Biosciences Influx high speed cell sorter |
| Microbial abundance, activity, and nutrient concentrations in Delaware Bay from R/V Hugh R. Sharp HRS090706DK in the Delaware Bay from July 2009 (Active bacteria in surface waters project) | | Flow Cytometer |
| Autotrophy, heterotrophy and niche partitioning in Caribbean sponges sampled June 9, 2019 on reef sites around Bocas del Toro Panama. | Cell counting was performed using a BD Biosciences Accuri C6 Plus Model flow cytometer at the Smithsonian Marine Station in Fort Pierce, Florida.
| BD Biosciences Accuri C6 Plus Model |
| Total bacteria, including Archaea and Prochlorococcus, by flow cytometry from R/V Thomas G. Thompson cruise TN277 in the Eastern North Pacific Ocean in 2012 (POWOW project) | A FACSCalibur flow cytometer (Becton Dickinson) was used to enumerate bacteria.
| Flow Cytometer |
| Bacteria Counts CTD Bottle Measurements from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022 | Samples were counted on an Accuri C6 Plus flow cytometer.
| Accuri C6 Plus flow cytometer |
| Bacterial cell counts during CDOM monoculture experiment | Used to make cell counts.
| FACSCalibur flow cytometer (Becton-Dickson) |
| Bacterial and viral cell counts, and nutrients from 5 cruises on the R/V Hugh R. Sharp in Delaware and Chesapeake Bays, 2014-2016 (Coastal Bacterial Growth Rates project) | Viruses were counted using a flow cytometer at Xiamen University, following their published method: Horizontal and Vertical Distribution of Marine Virioplankton: A Basin Scale Investigation Based on a Global Cruise. Y. Liang, et al. PLOS ONE www.plosone.org November 2014 9:11; e111634
| Flow Cytometer |
| Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) | Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation.
| Beckman-Coulter Altra flow cytometer |
| Specific daily growth of heterotrophic bacteria and grazing mortality on bacteria by microzooplankton from R/V Roger Revelle KIWI6, KIWI7, KIWI8, KIWI9 cruises in the Southern Ocean, 1997-1998 (U.S. JGOFS AESOPS project) | Coulter EPICS 753 flow cytometer with two lasers (UV and 488 nm)
| Coulter EPICS 753 flow cytometer |
| Bacteria cell counts and virus-like particle abundances from Espelandsvegen Fjord, Bergen Norway, May 2017 | Use to make cell counts of bacteria and virus particles.
| Influx Model 209S Mariner Flow Cytometer |
| Biogeochemical and microbial parameters collected during spatial surveys on a coral reef at Mo'orea's North Shore (French Polynesia) in September 2017, May 2019, and April 2022 | | Attune Acoustic Focusing Cytometer |
| Survey biogeochemical data from R/V Atlantis AT32, AT34, AT38, and AT39-06 in the western North Atlantic Ocean (35°N to 57°N; 45°W) in Nov. 2015, May 2016, Sep 2017, Mar/Apr 2018 | Used to measure bacterial abundance.
Flow cytometer equipped with a high throughput sampler, coherent sapphire 488nm laser and a default suite of six detectors (side-scatter and forward-scatter photodiodes and green, orange, red and far-red photomultipliers).
| BD LSR II equipped with a BD High Throughput Sampler (HTS) - Biosciences, San Jose, CA, USA |
| Algae, bacteria, DOC, inorganic and organic nutrients collected from the Richard B Gump Research Station at Moorea LTER from 2010-2011 (MCR LTER and Coral DOM projects) | | Flow Cytometer |
| Phytoplankton and bacteria abundance from flow cytometry from samples collected in the Gulf of Mexico on R/V Nancy Foster cruises NF1704 and NF1802 in May 2017 and May 2018 | The flow cytometer used is a Beckman-Coulter Altra (operated by the SOEST Flow Cytometry Facility, www.soest.hawaii.edu/sfcf), mated to a Harvard Apparatus syringe pump for quantitative analyses, and it is equipped with two argon ion lasers, tuned to UV (200 mW) and 488 nm (1 W) excitation. Scatter (side and forward) and fluorescence signals are collected using filters as appropriate, including those for Hoechst-bound DNA, phycoerythrin and chlorophyll.
| Beckman-Coulter Altra |
| Abundances of phytoplankton and non-pigmented bacteria determined by flow cytometry from water samples collected on R/V Roger Revelle cruise RR2201 in the Eastern Indian Ocean during February and March 2022 | Samples were analyzed with a Beckman Coulter CytoFlex S flow cytometer with a 96-well sample plate and 4 lasers (375 nm, 405 nm, 488 nm, and 536 nm).
| Beckman Coulter CytoFlex S |
| Coastal eastern subtropical pacific | | Millipore Guava EasyCyte 5HT flow cytometer |
| Flux of organic carbon for sponges at Conch Reef, Key Largo, FL, and Carrie Bow Cay, Belize as sampled in 2016. | | BD FACSCelesta Flow Cytometer |
| Sequence accession numbers for coral acid rich proteins (CARPs) from Stylophora pistillata; analyzed in the Falkowski lab at Rutgers from 2010-2014 (CROA project) | Flow cytometry analysis was performed on cells from different interfaces using a BD Influx Mariner 209S Flow Cytometer. Total genomic DNA of the Symbiodinium spp.-free cell fraction was extracted from harvested cells using a blood and cell culture DNA mini kit (Qiagen) with small modifications.
| BD Influx Mariner 209S Flow Cytometer |
| Cell abundance, forward scatter, chlorophyll, growth rate, and FvFm.0 measurements from reciprocal transplant assays | Guava easyCyte flow cytometer (Luminex Corporation, Austin, TX)
| Guava easyCyte flow cytometer |
| Phytoplankton and heterotrophic bacterial cell abundance over the incubation period in bioassay experiments with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019 | Cell abundance of both phytoplankton and heterotrophic bacterial cell abundance were determined using the Guava EasyCyte HT flow cytometer (Millipore).
| Guava EasyCyte HT flow cytometer (Millipore) |
| Cell counts from hadopelagic samples in the Mariana and Kermadec trenches, collected on R/V Falkor FK141109, FK141215, and R/V Thompson TN309, 2014 (Mariana Perspectives project) | | Attune Acoustic Focusing Flow Cytometer, Applied Biosystems, Foster City, CA |
| Prochlorococcus and Synechococcus cell counts in dilution experiment treatments from samples collected on RV Cape Hatteras cruises CH0409 and CH0510 in 2009 and 2010. | Used to analyze preserved samples
| Coulter-EPICS 753 flow cytometer |
| Chemotaxis of Pseudoalteromonas haloplanktis towards exudates of phage-infected and control Synechoccocus (VIC project) | Culture growth was tracked using a SpectraMax ID3 plate reader (Molecular Devices) and cell counts were measured using a CytoFLEX flow cytometer (Beckman Coulter).
| CytoFLEX flow cytometer (Beckman Coulter) |
| Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells (VIC project) | Culture growth was tracked using a SpectraMax ID3 plate reader (Molecular Devices) and cell counts were measured using a CytoFLEX flow cytometer (Beckman Coulter).
| CytoFLEX flow cytometer (Beckman Coulter) |
| Physiochemical data from samples collected along the coast of Louisiana, USA during 2018 | Used for sampling
| Millipore Guava 5HT HPL benchtop flow cytometer |
| Bacterial transcriptional response to picoeukaryote Micromonas commoda | Agilent Quanteon flow cytometer (Acea, Biosciences Inc, San Diego CA) with a 405 nm laser using a 530/30 bandpass filter for SYBR Green for bacterial cell counts. 405 nm laser using a 695/40 bandpass filter for chlorophyll a (phytoplankton) was used for micromonas cell counts.
| Agilent Quanteon flow cytometer (Acea, Biosciences Inc, San Diego CA) |
| Physiology measurements on collected coral pairs from Kaneohe Bay, Oahu, HI between July and December 2019 | | Millipore Guava flow-cytometer (Guava easyCyte 5HT) |
| Diel, daily, and spatial variation of coral reef seawater microbial communities from US Virgin Islands, 2017 | Used for measuring cell concentrations. Samples collected for flow cytometry were analyzed using colinear analysis (laser excitation wavelength of 488 nm, UV) on an Altra flow cytometer (Beckman Coulter, Pasadena, CA.).
| |
| Growth behaviors of diatom monoculture and diatom-bacteria co-cultures in the lab | | Guava |
| 3A: Removal of organic carbon by natural bacterioplankton communities as a function of pCO2 from laboratory experiments between 2012 and 2016 | Flow Cytometry (FCM) on an LSR II with SYBR Green I according to Nelson et al. 2011.
| Flow Cytometry (FCM) |
| Bacterial abundance, bacterial organic carbon, and total organic carbon from remineralization bioassays conducted on R/V Robert Gordon Sproul cruises along the Southern California coast during July and August 2023 | Bacterial cells were stained with SYBR Green I (ThermoFisher) and enumerated on a Guava flow cytometer (Millipore) (Gasol et al., 2016).
| Guava flow cytometer |
| Population changes in Halobacteriovorax cultured with protist & prey | CytoFLEX Flow Cytometer (Beckman Coulter Life Sciences Headquarters, Indianapolis, IN 46268, USA)
| CytoFLEX Flow Cytometer |
| Carbonate data in experimental treatments of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project) | BD FACS Calibur flow cytometer
| Flow Cytometer |
| Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| Flow Cytometer |
| Carbon and nitrogen content of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| Flow Cytometer |
| Experimental results: Emiliania huxleyi growth rates under different pCO2 levels from Bellingham, WA from 2011-2012 (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| Flow Cytometer |
| Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| Flow Cytometer |
| Experimental results: Emiliania huxleyi growth rates under different pCO2 levels, 2011-2012 (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| Flow Cytometer |
| SEM based morphology of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project) | BD FACS Calibur flow cytometer
| Flow Cytometer |
| Taxonomy and abundance of microbial cells (phytoplankon and heterotrophic bacteria) during a deployment of the Environmental Sample Processor (ESP) in Fall, 2014 in Monterey Bay, CA | Simultaneously detects Hoechst stain (DNA), cell pigments, and forward and side (90 degrees) scatter.
| Beckman Coulter Altra flow |
| Eukaryotic phytoplankton flow cytometric results from salp grazing incubations conducted on R/V Tangaroa cruise TAN1810 during Oct-Nov 2018 | Becton-Dickinson Accuri C6 Plus flow cytometer
| |
| Physiological metrics recorded at the end of experiments conducted to examine two coral species' responses to thermal stress | | flow cytometer (BD LSRFortessa, BD Biosciences) |
| Direct counts (flow cytometry) on microbes obtained by Niskin bottle and pressure-retaining sampler from the Leggo Lander on R/V Falkor cruise FK141215 in the Challenger Deep, Mariana Trench in December 2014 | | |
| Results of the quantification of symbiont cell numbers from 400 Acropora hyacinthus colonies subjected to experimental bleaching in the summer of 2018 in Palau. | | Guava easyCyte HT 2-laser flow cytometer (Millipore) |
| Flow Cytometry Results from Barbados incubation experiment, February 2012 (ADIMA project) | Approximately 10,000 0.75 m beads (FluoresbriteTM particles, YG, Polysciences, Inc.) were added into each sample and samples were analyzed by flow-cytometer (BD Influx cell sorter) triggering on forward angle light scatter (FSC) to determine total cell numbers of populations of Prochlorococcus, Synechococcus and pico-eukaryotic algae.
| Flow Cytometer |
| Maximum potential quantum efficiency of Photosystem II (Fv/Fm) for two coral species, Acropora hemprichii and Porites lobata, in response to thermal stress | | flow cytometer (BD LSRFortessa, BD Biosciences) |
| Phytoplankton carbon biomass by taxa from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018 | | Beckman Coulter EPICS Altra flow cytometer |
| Pigment data by phytoplankton taxa from CTD casts from NOAA Ship R/V Nancy Foster cruises NF1704 and NF1802 in the Gulf of Mexico, May 2017 and 2018 | | Beckman Coulter EPICS Altra flow cytometer |
| Daily cell count data for low to high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project) | BD FACSCalibur flow cytometer
| |
| Flow cytometric counts from grazing saturation culture experiment using single prey (Micromonas pusilla) and predator (Ochromonas danica) in October 2019 | A ZE5 Cell Analyzer (Bio-Rad, Hercules, CA, USA) was used to measure optical properties and abundance of single cells from each sample
| Bio-Rad ZE5 Cell Analyzer |
| Flow cytometric counts from grazing saturation culture experiment using single prey (Isochrysis galbana) and predator (Ochromonas danica) from March to April 2020 | A ZE5 Cell Analyzer (Bio-Rad, Hercules, CA, USA) was used to measure optical properties and abundance of single cells from each sample
| Bio-Rad ZE5 Cell Analyzer |
| Flow cytometry measurements from HHQ experiments conducted during the MesoHux mesocosm experiment, May 2017, Bergen, Norway | Used for cell counts
| Millipore Guava inCyte BG HT flow cytometer |
| Flow cytometer cell counts from R/V Kilo Moana KM1513 (HOE-LEGACY 2A ) near Hawaii (22.75 N, 158 W) from July to August 2015 (C-MORE project, SCOPE project) | Flow Cytometer
| Flow Cytometer |
| Hydrolytic enzyme activities during CDOM monoculture experiment with Coscinodiscus | Used to make cell counts.
| FACSCalibur flow cytometer (Becton-Dickson) |
| Hydrolytic enzyme activities during CDOM monoculture experiments with Skeletonema, Leptocylindrus, and Phaeocystis | Used to make cell counts.
| FACSCalibur flow cytometer (Becton-Dickson) |
| Initial prey abundances for copepod grazing experiments in the Kaneohe Bay, HI, May-June 2013 (MEPS 2017) (EAGER: Copepod nauplii project) | | Beckman Coulter Altra flow cytometer |
| Phytoplankton diversity along with carbon and nitrogen uptake rates collected along the GO-SHIP IO9 repeat hydrography section of the Indian Ocean from R/V Roger Revelle cruise RR1604 from March to April 2016 | Cell counts for heterotrophic bacteria smaller ( | BD FACSJazz flow cytometer |
| Bacteria and virus abundance data collected from the R/V Melville MV1405 along the California coastline during 2014 | Samples analyzed on flow cytometer using BD Software (BD Biosciences).
| Influx Model 209S Mariner Flow Cytometer |
| Metadata for field dilution experiments to measure community microzooplankton grazing rates in Kaneohe Bay, HI from 2012-2013 (EAGER: Copepod nauplii project) | | |
| Field conditions during grazing experiments in Kaneohe Bay, HI during 2012-2013 (EAGER: Copepod nauplii project) | | |
| Flow cytometry, 16S rRNA gene amplicons, chlorophyll a, and surface seawater measurements taken between August 2017 to June 2019 Kāneʻohe Bay, Oʻahu, Hawaiʻi | | EPICS ALTRA flow cytometer (Beckman Coulter Inc., Brea, CA, USA |
| Biological oceanographic measurements, 16S rRNA gene amplicons and metagenomes from surface seawater taken from August 2017 to June 2021 at sites within and adjacent to Kāneʻohe Bay, Oʻahu, Hawaiʻi | | Beckman Coulter CytoFLEX S |
| Flow cytometry results for naupliar grazing laboratory experiments conducted from 2012-2013 (EAGER: Copepod nauplii project) | | |
| Body length, right postoral rod length, and stomach length of Dendraster excentricus and Lytechinus pictus larvae raised at three culture densities on two food rations from 2021-2022 (LIPs on Larval Feeding project) | A BD Accuri C6 Flow Cytometer (BD Biosciences), was used to determine the concentration of Rhodomonas lens.
| BD Accuri C6 Flow Cytometer (BD Biosciences) |
| Microbial abundance of phytoplankton and bacteria from RV/Tangaroa cruise TAN1810SALP to Chatham Rise vicinity, east of New Zealand, Oct - Nov. 2018 | Used for shore-based cell counts
| Beckman Coulter CytoFLEX S, with Near UV (375 nm), Violet (405 nm), Blue (488 nm), and Yellow-Green (561 nm) lasers, with 96-well plate loader |
| Microbial abundance of phytoplankton and bacteria from RV/Tangaroa cruise TAN1810SALP to Chatham Rise vicinity, east of New Zealand, Oct - Nov. 2018 | Used for cell counts on board ship.
| Becton Dickinson Accuri C6 Plus with CSampler Plus |
| Microbiome dynamics of coral reef and cleanerfish from ecological surveys, in situ manipulations, and laboratory experiments conducted from 2020-2021 | | Altra flow cytometer (Beckman Coulter) |
| Virioplankton abundance using FISH probe at BATS site in the western Sargasso Sea from 2000-2011 (Ocean Microbial Observatory project) | Becton Dickenson (Franklin Lakes, NJ, USA; formerly Cytopeia) high speed jet-in-air InFlux flow cytometer, using a 488 nm blue excitation laser, appropriate Chl-a (692±20 nm) and phycoerythrin (580±15 nm) bandpass filters.
| Flow Cytometer |
| Virioplankton abundance from multiple cruises at the Bermuda AtlanticTime Series Station (BATS), Western Sargasso Sea from 2000-2011 (Ocean Microbial Observatory project) | Becton Dickenson (Franklin Lakes, NJ, USA; formerly Cytopeia) high speed jet-in-air InFlux flow cytometer, using a 488 nm blue excitation laser, appropriate Chl-a (692±20 nm) and phycoerythrin (580±15 nm) bandpass filters.
| Flow Cytometer |
| Abundance of bacteria viruses and chlorophyll containing cells collected from the R/V Oceanus OC1504A in the Oregon/California Coastal Upwelling Zone, between 34-44N and 120-124W during 2015 | Samples were analyzed on flow cytometer using BD Software (BD Biosciences). Bacterial and viral abundances were analyzed on this flow cytometer.
| Influx Model 209S Mariner Flow Cytometer |
| Abundance of bacteria viruses and chlorophyll containing cells collected from the R/V Oceanus OC1504A in the Oregon/California Coastal Upwelling Zone, between 34-44N and 120-124W during 2015 | Chlorophyll containing cells analyzed on this flow cytometer.
| BD Accuri C6 |
| Flow cytometric counts of picoeukaryotes, Synechococcus, and beads using natural waters from the Gulf of Maine during Jul-Aug 2019 and Jun-Jul 2021 | A ZE5 Cell Analyzer (Bio-Rad, Hercules, CA, USA) was used to measure optical properties and abundance of single cells from each sample
| Bio-Rad ZE5 Cell Analyzer |
| Copepods Parvocalanus crassirostris and Bestiolina similis naupliar ingestion and clearance rates on natural prey assemblages from Kaneohe Bay, Oahu, 2013 (MEPS 2017) (EAGER: Copepod nauplii project) | For photosynthetic eukaryote (PEUK) abundance
| |
| Male fecundity following parental ocean acidification during lab experiments conducted in spring 2022. | GuavaⓇ easyCyte™ HT flow cytometer (MilleporeSigma, St. Louis, MO, USA) for sperm concentration determination
| GuavaⓇ easyCyte™ HT flow cytometer (MilleporeSigma, St. Louis, MO, USA) |
| Depth profile data from R/V New Horizons NH1418 in the tropical Pacific from Sept-Oct. 2014 | Used for cell counts
| Influx Flow Cytometer |
| Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA | | Beckman Coulter Altra |
| Normalized oxygen from incubations conducted with methylcyclohexane using samples collected from the Gulf of Mexico during June 2015 on R/V Atlantis cruise AT29-02 | | Millipore Guava EasyCyte 5HT Flow Cytometer |
| Normalized oxygen from incubations conducted with methylcyclopentane using samples collected from the Gulf of Mexico during June 2015 on R/V Atlantis cruise AT29-02 | | Millipore Guava EasyCyte 5HT Flow Cytometer |
| Response of Bathycoccus sp. to iron-limitation sampled aboard R/V Roger Revelle RR1813 in the Subarctic North Pacific near Station PAPA (50 N, 144.8 W) from August to September 2018 | | Guava easyCyte flow cytometer |
| Nutrient and cell count data from incubations conducted with methylcyclohexane or methylcyclopentane on samples collected from the Gulf of Mexico during June 2015 on R/V Atlantis cruise AT29-02 | | Millipore Guava EasyCyte 5HT Flow Cytometer |
| Nutrients and flow cytometry from station N-1200 collected in August 2017 from a cruise aboard R/V Mediterranean Explorer | | BD Canto II flow-cytometer |
| Flow cytometry cell counts obtained during the R/V L'Atalante OUTPACE cruise between New Caledonia and Tahiti from February to April 2015 | Instrument Type: Flow cytometer (Manufacturer: BD) Model: Influx
| Influx Flow cytometer |
| Phenotypic plasticity of the ciliated band of seven species of echinoderm larvae, collected between 2020 and 2022 in the laboratory at California State University, Long Beach. | BD Accuri C6 Flow Cytometer (BD Biosciences) was used to determine concentration of Rhodomonas lens for larval feeding.
| BD Accuri C6 Flow Cytometer (BD Biosciences) |
| Phosphohydrolysis rates from samples collected in the coastal western North Atlantic on R/V Endeavor cruise EN588 during September 2016 | | Guava EasyCyte HT flow cytometer (Millipore) |
| Biogeochemistry of microbial phosphorus uptake from cruises in the Sargasso Sea; Bermuda Atlantic Time-Series Station from 2011-2013 (Biological C:N:P ratios project) | InFlux cell sorter (BD, Seattle, WA)
| Flow Cytometer |
| Phosphorus uptake kinetics by microbes to whole communities from R/V Atlantic Explorer AE1206, AE1319 in the Sargasso Sea; Bermuda Atlantic Time-Series Station from 2012-2013 (Biological C:N:P ratios project) | InFlux cell sorter (BD, Seattle, WA)
| Flow Cytometer |
| Taxon-specific phosphorus uptake by microbes from NW Atlantic and western Sargasso Sea from 2007-2013 (Biological C:N:P ratios project) | InFlux cell sorter (BD, Seattle, WA)
| Flow Cytometer |
| Photosynthesis and respiration data recorded during experiments conducted to examine two coral species' responses to thermal stress | | flow cytometer (BD LSRFortessa, BD Biosciences) |
| Physiological responses of the coral host and associated endosymbionts measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | MACSQuant Analyzer 10, Miltenyi Biotec |
| Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻi | endosymbiont densities: Millipore Guava flow-cytometer (Guava easyCyte 5HT)
| Guava easyCyte 5HT |
| Phytoplankton growth and grazing from flow cytometry in the eastern Indian Ocean | | Beckman-Coulter CytoFLEX-S Flow Cytometer |
| Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) determined by flow cytometry of samples collected on R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project) | A FACSCalibur flow cytometer (Becton Dickinson) was used to enumerate phytoplankton.
| Flow Cytometer |
| Phytoplankton and heterotrophic bacterial cell abundance in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019 | Cell abundance of both phytoplankton and heterotrophic bacteria were determined using the Guava EasyCyte HT flow cytometer (Millipore).
| Guava EasyCyte HT flow cytometer (Millipore) |
| Cell abundance, growth rate, cellular P quotas, and alkaline phosphatase activity from a laboratory experiment examining the response of three species of marine phytoplankton grown under different phosphorus (P) conditions | | BD FACSJazz cell sorter |
| Phytoplankton abundances from experiments on R/V Seward Johnson SJ0516 cruise between Ireland and Iceland during the 2005 North Atlantic Spring Bloom (NASB 2005 project, Antarctic microzooplankton project) | Samples were analyzed in the laboratory on a Becton Dickinson FACSCalibur benchtop flow cytometer.
| Flow Cytometer |
| Phytoplankton data from phyto and microzooplankton experiments from the RVIB Nathaniel B. Palmer NBP0601 cruise in the Ross Sea, Southern Ocean from 2005-2006 (CORSACS project, Antarctic microzooplankton project) | Samples were run on a FACSCalibur flow cytometer.
| Flow Cytometer |
| Pico- and Nanoplankton concentrations from CTD cast deployments collected from the R/V Endeavor (EN532, EN538) cruises in the subarctic Atlantic Ocean from 2013-2014 | BD Accuri C6 flow cytometer
| |
| Diel series of pico-cyanobacteria concentration and cell properties from the RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010. | Used to analyze preserved samples
| Coulter-EPICS 753 flow cytometer |
| Picocyanobacteria (Prochlorococcus or Synechococcus) and picoeukaryote (Micromonas and Ostreococcus) cell concentrations and hydrogen peroxide (HOOH) concentrations during batch culture at the University of Tennessee, Knoxville | The flow cytometer passes liquid in a narrow stream through the path of light emitted by a laser. Detectors can measure light scatter and fluorescence generated when the photons interact with particles (e.g. cells) moving in the stream. Phytoplankon can be distinguished by the fluorescence profiles established by their suite of fluorescent photopigments.
| CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA) |
| Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) | Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation.
| Beckman-Coulter Altra flow cytometer |
| Picophytoplankton abundances of live samples determined by flow cytometry (shipboard analysis) on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) | Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser for excitation.
| Beckman-Coulter Altra flow cytometer |
| Picoplankton Abundance Data from R/V Roger Revelle KNOX22RR in the Patagonian Shelf (SW South Atlantic) from December 2008 (COPAS08 project) | | Flow Cytometer |
| Plankton size spectra compiled from projects CCE LTER, HOT, CRD FluZIE, BLOOFINZ-GoM, and SalpPOOP in the California Current Ecosystem, North Pacific subtropical gyre, Costa Rica Dome, Gulf of Mexico, and Southern Ocean subtropical front from 2004-2018 | There were multiple different flow cytometers used. Typically a seagoing flow cytometer was used for picoeukaryotes and a higher resolution flow cytometer was used for bacteria. The flow cytometers varied between studies. Data users can go to the cited publications (or original flow cytometry datasets) to find out more.
| Flow Cytometer |
| Growth rate of Pleurochrysis carterae CCMP645 cells as measured by flow cytometry in laboratory experiments | Host abundance in each flask was monitored by flow cytometry (FCM) on a BD FACScan(TM) System for a 106-day period.
| BD FACScan(TM) System |
| Prochlorococcus or Synechococcus cell concentrations and nitrite concentrations during batch culture with ammonium or nitrate as the sole nitrogen source for growth | | Guava easyCyte 12HT Flow Cytometer (MilliporeSigma, Burlington, MA, USA) |
| Prochlorococcus in situ cell cycle phases fractions from RV Cape Hatteras cruises CH0409 and CH0510 in the Western Sargasso Sea in 2009 and 2010. | Used to analyze preserved samples
| Coulter-EPICS 753 flow cytometer |
| Prochlorococcus N and C uptake from R/V Atlantic Explorer AE1032 in the Western Subtropical North Atlantic from Oct. 2010 (Nitrogen uptake in Prochlorococcus project) | | Flow Cytometer |
| The abundance of Prochlorococcus cells containing the nitrate reductase gene (narB) at the HOT and BATS sites in the Pacific and Atlantic Oceans between October 2005 and January 2008 | from Becton Dickinson, Franklin Lakes, NJ, USA
| influx flow cytometer |
| Ammonium, total nitrate and nitrite, nitrite, and flow cytometry profiles in the Eastern Tropical North Pacific from March to April 2018 | A BD Accuri C6 Flow Cytometer was used for the enumeration.
| BD Accuri C6 Flow Cytometer |
| Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1 from R/V Falkor cruise FK141215 in the Challenger Deep, Mariana Trench in December 2014 | Cells were sorted using a flow cytometer
| |
| Quantification of ciliated band length per unit protein in early echinoderm larvae (biometirc data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. | BD Accuri C6 Flow Cytometer (BD Biosciences) was used to determine concentration of Rhodomonas lens for larval feeding.
| BD Accuri C6 Flow Cytometer (BD Biosciences) |
| Quantification of ciliated band length per unit protein in early echinoderm larvae (protein data), collected between 2020 and 2022 in the laboratory at California State University, Long Beach. | BD Accuri C6 Flow Cytometer (BD Biosciences) was used to determine concentration of Rhodomonas lens for larval feeding.
| BD Accuri C6 Flow Cytometer (BD Biosciences) |
| Coral physiology data (chlorophyll a, symbiont densities) on four collected coral species from six sites in the Red Sea from 2018 to 2020 | | flow cytometer (BD LSRFortessa, BD Biosciences) |
| Biogeochemistry, metabolomics, and metagenomics of Florida's Coral Reef from sampling conducted over 15 days in June 2019 | Beckman-Coulter Altra flow cytometer (Beckman Coulter Life Sciences) for enumerating cell abundances
| Beckman-Coulter Altra flow cytometer |
| Reef seawater biogeochemistry data from samples collected in the Jardines de la Reina reef-system, Cuba in November of 2017 | To obtain cell counts, flow cytometry was conducted using a collinear analyses method and a UV wavelength of 488 nm on an Altra flow cytometer at the University of Hawaii. Each sample was divided so that pigmented, fluorescent cells and unpigmented cells could be run separately.
| Altra flow cytometer |
| Optical density and cell counts from flow cytometry of Ruegeria pomeroyi laboratory cultures | | Guava EasyCyte flow cytometer (Luminex) |
| Plankton cell abundances and APA data from RV Atlantic Explorer BATS cruise BV50 in the North Atlantic subtropical gyre during September 2015. | Used for flow cytometry analyses
| BD Influx flow cytometer |
| Bulk and cell-specific CO2 fixation and PO4 uptake from Atlantic Explorer cruise AE1524 (BATS validation cruise BV50), September 2015 | Used for flow cytometry analyses
| BD Influx flow cytometer |
| Hydrocarbon concentrations, DIC isotopes, nutrients, and cyanobacteria counts from samples collected on R/V Neil Armstrong cruise AR16 in the western north Atlantic during May 2017 | | Influx cytometer |
| Dilution batch-culture bioassay (remineralization) experiments from SBDOM11 project cruise from R/V Point Sur PS1103 in the Santa Barbara Channel, May 2011 (SBDOM project, SBC LTER project) | BD LSRII flow cytometer with an autosampler attachment used to obtain bacterial abundance.
| BD LSRII flow cytometer |
| Seawater nutrient/metabolite and flow cytometry metadata - Picoplankton incubation experiment 2020 | | Bio-Rad ZE5, Hercules, California, USA |
| Series 1B-2: Multiple stressor experiments on T. pseudonana (CCMP1335) – cell abundance and cell size in experiments | Used for measuring abundance and forward scatter (proxy for cell size)
| Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA) |
| Series 2A-1: Multiple stressor experiments on T. pseudonana (CCMP1014) – cell abundance and cell size in experiments | Used for measuring abundance and forward scatter (proxy for cell size)
| Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA) |
| Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – cell abundance by flow cytometry | Used to measure abundance and forward scatter (proxy for cell size).
| Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA) |
| Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - cell size estimated by forward scatter from flow cytometry | Used to measure abundance and forward scatter (proxy for cell size).
| Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA) |
| Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) - growth under bubbling stress: flow cytometry measurements | Used to count cells.
| Guava easyCyte HT Sampling Flow Cytometer |
| Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: computed photochemical measurements using LC3 protocol for non-aerated samples and aerated samples | Used to count cells.
| Guava easyCyte HT Sampling Flow Cytometer |
| Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: LC3 protocol raw fluorescence measurements for non-aerated samples and aerated samples | Used to count cells.
| Guava easyCyte HT Sampling Flow Cytometer |
| Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching) computed photochemical measurements | Used to count cells.
| Guava easyCyte HT Sampling Flow Cytometer |
| Series 3B: Supplemental experiments on T. pseudonana (CCMP1014) growth under bubbling stress: NPQ1 protocol (Non-Photochemical chlorophyll fluorescence Quenching) raw fluorescence measurements for non-aerated samples and aerated samples | Used to count cells.
| Guava easyCyte HT Sampling Flow Cytometer |
| Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 – cell abundance and size by flow cytometry | Used to measure abundance and forward scatter (proxy for cell size).
| Guava easyCyte HT Benchtop Flow Cytometer (Millipore-Sigma, USA) |
| Counts of picocyanobacteria from SMILE cruise (SKQ201703S) at the North Pacific Subtropical Front in March 2017 | | BD Biosciences Influx high speed cell sorter |
| Oceanographic data from 12 monthly cruises off the Israeli coast in the east Mediterranean conducted between Feb 2018 and Jan 2019 at a continental shelf station (~140m depth) and an open-deep water station. | BD Canto II flow-cytometer with 2 μm diameter fluorescent beads (Polysciences, Warminster, PA, USA)
| BD Canto II flow-cytometer |
| Direct measure of phytoplankton cell quotas from field populations sampled from multiple cruises cruises between 2010 and 2016 | Becton Dickinson Influx or FACSJazz flow cytometer, each utilizing a 200 mW 488 nm laser, with detectors for forward scatter, side scatter, 692 nm, and 530 nm
| |
| Microbial cell counts in seawater samples collected on R/V Nerissa and R/V Yellowfin cruises at the San Pedro Ocean Time-series (SPOT) in 2017 | Autotrophic picoplankton (Prochlorococcus, Synechococcus, and picoeukaryotes) and heterotrophic prokaryotes were enumerated by flow cytometry (Becton Dickinson FACSCalibur).
| Becton Dickinson FACSCalibur |
| Cell abundance, nutrient and DMSP concentrations measured during a mesocosm study of the effect of phytoplankton composition on bacterial DMSP transformation (OceanSulfurFluxBact project) | To measure abundance of cells.
| flow cytometer |
| Data on laboratory cultures and statistical analysis code associated with the paper "Co-culture with Synechococcus facilitates the growth of Prochlorococcus under ocean acidification conditions" published in Environmental Microbiology | Most data in this study were collected using a Luminex Guava HT-1 flow cytometer.
| Luminex Guava HT-1 flow cytometer |
| Synechococcus batch culture data (cell quotas and ratios (C,N,P), size, and diameter) from laboratory experiments in 2021 to 2022 with related isolates cultured across a range of temperatures (16-25C) | | NovoCyte 1000 (Agilent, Santa Clara, CA) |
| Synechococcus (WH8102 and CC9311) growth and genetic sequence accessions from experiments with variable pCO2 treatments from 2016 to 2018 | | Millipore Guava HT1 Flow Cytometer |
| Temperature data measured during two experiments testing the effects of acute and chronic thermal stress on the scleractinian coral, Stylophora pistillata | | MACSQuant Analyzer 10, Miltenyi Biotec |
| Data and code from an examination of growth rates of cyanobacteria co-cultured with a heterotrophic bacterium, Alteromonas, under either present-day or predicted future pCO2 conditions | | Guava HT1 flow cytometer with 488nm laser |
| Data and analysis code used to experimentally evolve representatives of four phytoplankton functional types in co-culture with a heterotrophic bacterium under either present-day or predicted future pCO2 conditions | | Guava HT1 flow cytometer (Luminex) |
| Flow cytometry and nutrient analyses data from a tidal study over 48 hours of mangrove, seagrass, and seawater from the US Virgin Islands in July of 2017 | | EPICS Altra flow cytometry (Beckman Coulter Life Sciences, Inc, Indianapolis, IN) |
| Coastal water biogeochemistry collected aboard the R/V Endeavor along the North Atlantic coast from 2017-08-20 to 2017-08-28 | Bacteria and group-specific phytoplankton counts were conducted on a Guava EasyCyte HT flow cytometer (Millipore).
| Guava EasyCyte HT flow cytometer (Millipore) |
| Transcriptomics of phytoplankton cultures grown on various phosphorus sources in a laboratory experiment | | flow cytometry |
| Seawater microbial communities within coral reef seawater change over six years in response to disturbance | | Beckman-Coulter Altra flow cytometer |
| Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023 | FACS Aria III flow cytometer: The instrument was used to enumerate prokarytes and viruses after staining with the dsDNA-specific stain SYBR Green I.
| FACS Aria III flow cytometer |
| Bacteria, picoeukaryote, and Synecoccus cell counts and chlorophyll-a from nitrate and vitamin-B treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project) | Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company).
| BD Accuri C6 flow cytometer (Becton Dickerson and Company) |
| Bacteria, picoeukaryote, and Synecoccus cell counts, nutrients (POC, PON, POP) and chlorophyll from nitrate and vitamin-B enriched treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project) | Used for cell counts. Samples for flow cytometry were collected, fixed with 2% formalin, and frozen at -80C. Analysis for the cellular abundance of heterotrophic bacteria, Synechococcus, and pircoeukaryotes was conducted using a BD Accuri C6 flow cytometer (Becton Dickerson and Company).
| BD Accuri C6 flow cytometer (Becton Dickerson and Company) |
| Surface water and N.digitalis inhalent/exhalent water sample nutrient and bacterioplankton metadata of Looe Key and Wonderland Reef conducted in 10-15 December 2023 | Preserved samples were analyzed using a Bio-Rad ZE5 with 405 nm, 488 nm, and 640 nm lasers activated.
| Bio-Rad ZE5 |
| Depth profiles of oceanographic parameters in spring and summer from R/V Terrapin, R/V Hugh R. Sharp multiple cruises in Chesapeake Bay, 2010-2011 (LiDZ project) | Enumeration was performed using a FACSCalibur flow cytometer (Becton Dickinson) using green fluorescence as an incident trigger, with a target range for sample event rates at 100-1000 particles s-1 to prevent incident coincidence.
(http://www.bdbiosciences.com/instruments/facscalibur/features/index.jsp)
| Flow Cytometer |
| Chlorophyll a and flow cytometry data from bi-weekly vertical profiles in Resurrection Bay, AK from January to March of 2023 from bi-weekly vertical profiles in Resurrection Bay, AK from January to March of 2023 | | Beckman Coulter CytoFLEX S flow cytometer |
©2020 Biological and Chemical Oceanography Data Management Office.