Instrument: Flow Cytometer

Acronym:

Flow Cytometer

External Identifier:

» skos:broadMatch http://vocab.nerc.ac.uk/collection/L05/current/LAB37/

Description

Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)

Datasets

Dataset Name	Brief Description
13C incubation cell counts	Counts of Prochlorococcus from on-deck incubations with 13C-bicarbonate as part of DNA-SIP experiments
active surface bacteria	Microbial abundance, activity, and nutrient concentrations in Delaware Bay
bacteria	Total bacteria, including Archaea and Prochlorococcus, by flow cytometry; data from POWOW1 cruise.
Bacterial cell counts	Bacterial cell counts during CDOM monoculture experiment
Bacterial Growth ACTivity	New data and new versions of previous data: Bacterial growth rate ACTivity in Delaware Bay
bacteria_FCM_lab	Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis).
Bacterivory	Bacterivory
Bergen MesoHux 2017 Bacteria and Virus Abundance	Bergen MesoHux 2017 Bacteria and Virus Abundance
Biogeochemical survey 2015-2018	Biogeochemical data from cruises AT32, AT34, AT38, AT39-06
biogeochem_microbes	Moorea Coral Reef algae, bacteria, DOC, inorganic and organic nutrients
BLOOFINZ-GoM flow cytometry abundance
BLOOFINZ-IO Flow Cytometry Abundance	BLOOFINZ-IO Flow Cytometry Abundance
Candidatus Nitrosopelagicus brevis strain U25
Carbon flux for Caribbean coral reef sponges
CARP accession numbers	Sequence accession numbers for coral acid rich proteins (CARPs) from Stylophora pistillata.
Cell abundance and chlorophyll content	Cell abundance and chlorophyll content
Cell Abundance Bioassay Experiment
Cell counts - water	Cell counts from Mariana and Kermadec trenches, 2014
Cell counts in dilution experiment	Prochlorococcus and Synechococcus cell counts in dilution experiment treatments.
Chemotaxis of P. haloplanktis towards exudates of Synechoccocus
Chemotaxis of V. alginolyticus towards Synechococcus Cells	Chemotaxis of Vibrio alginolyticus towards live phage-infected/control Synechococcus cells
Coastal SAR11 Physiochemical data	Physiochemical data from samples collected along the coast of Louisiana, USA during 2018.
Controlled laboratory study using model organisms Micromonas commoda RCC 299 and Ruegeria pomeroyi DSS-3	picoeukaryote Micromonas commoda
Coral Colony Physiological Summary
Coral reef seawater microbial communities	Coral reef seawater microbial communities
Culture Characteristics
Data Set 3A: Utilization of dissolved organic carbon by a natural bacterial community as a function of pCO2	Data Set #3A: Utilization of dissolved organic carbon by a natural bacterial community as a function of pCO2
Effects of protists on HBx predation	Population Changes
Emiliania huxleyi carbonate data	Carbonate data in experimental treatments of Emiliania huxleyi
Emiliania huxleyi Chl-a, POC, cell volumes	Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels
Emiliania huxleyi CN content	Carbon and nitrogen content of E. huxleyi at 3 pCO2 levels, 2011-2012
Emiliania huxleyi dilution calculations	Emiliania huxleyi growth rates under different pCO2 levels
Emiliania huxleyi DMSP	Cellular particulate DMSP from E. huxleyi at 3 pCO2 levels
Emiliania huxleyi growth rates	Emiliania huxleyi growth rates under different pCO2 levels
Emiliania huxleyi SEM	SEM based morphology of Emiliania huxleyi in 2011 and 2012
ESP 2014 Cell Counts	Microbial taxonomy and abundance from ESP, fall 2014
Eukaryotic Phytoplankton Flow Cytometry from Salp Grazing Incubations	Eukaryotic Phytoplankton Flow Cytometry from Salp Incubations
Final Physiology	Final Physiology
flow cytometry	Direct counts (flow cytometry) on microbes obtained by Niskin bottle and pressure-retaining sampler
Flow Cytometry Results
Flow Cytometry Results	Flow Cytometry Results
Fv/Fm	Fv/Fm
GoMex phytoplankton carbon biomass	Phytoplankton carbon biomass
GoMex pigments	CTD Fluorometer
Grazing experiment 2 and 3: daily cell counts	Daily cell count data for low to high pCO2 acclimated E. huxleyi
Grazing saturation culture experiment #1 using single prey (M.pusilla) and predator (O.danica)	Culture experiment (M.pusilla + O.danica)
Grazing saturation culture experiment #2 using single prey (I.galbana) and predator (O.danica)	Culture experiment (I.galbana + O.danica)
HHQ Flow Cytometry	HHQ Flow cytometry
HOE-LEGACY 2A diel_FCM	HOE-LEGACY 2A diel_FCM
Hydrolytic enzyme activity - Coscinodiscus	Bacterial cell counts during CDOM Coscinodiscus monoculture experiment
Hydrolytic enzyme activity - other phytoplankton	Bacterial cell counts during CDOM monoculture experiment
Initial prey abundance and biomass: MEPS 2017	Initial prey abundance and biomass: MEPS 2017
IO Phytoplankton	Phytoplankton diversity along with carbon and nitrogen uptake rates along the GO-SHIP IO9 repeat hydrography section of the Indian Ocean
IrnBru MV1405 - Bacteria and Virus Abundance	Bacteria and virus abundance data from the MV1405 cruise.
Kaneohe Bay microzooplankton growth and mortality	Metadata for field dilution experiments to measure community microzooplankton grazing rates
Kaneohe Bay physical conditions	Field conditions during grazing experiments
Kāneʻohe Bay Time-series - microbial community	Kāneʻohe Bay Time-series - microbial community
Kāneʻohe Bay Time-series - phytoplankton and biogeochemistry	Kāneʻohe Bay Time-series - phytoplankton and biogeochemistry
lab naupliar grazing: prey densities	Flow cytometry results for naupliar grazing experiments (laboratory)
Larvae Density Morphometrics	Body length, right postoral rod length, and stomach length of Dendraster excentricus and Lytechinus pictus larvae raised at three culture densities on two food rations.
Microbial abundance	Microbial abundance
Microbial abundance	Microbial abundance
Microbiome dynamics of coral reef and cleanerfish	Microbiome dynamics of coral reef and cleanerfish
MO - virioplankton abund-FISH probe	Virioplankton abundance at BATS site using FISH probe, 2003-2011
MO - virioplankton abundance	Virioplankton abundance at BATS site, 2000-2011
MUSiCC OC1504A - Bacteria Virus and Chlorophyll Containing Cell Abundance	Abundance of bacteria viruses and chlorophyll containing cells
MUSiCC OC1504A - Bacteria Virus and Chlorophyll Containing Cell Abundance	Abundance of bacteria viruses and chlorophyll containing cells
Natural waters experiment with picoeukaryotes and Synechococcus	Natural waters experiment (picoeukaryotes + Synechococcus)
Naupliar ingestion: MEPS 2017	Naupliar grazing by Parvocalanus crassirostris and Bestilina similis nauplii (uncorrected rates)
Nematostella ocean acidification experiment: Male Fecundity	OA experiment: Nematostella male fecundity
NH1418: CTD profiles	NH1418 ctd
Niskin 2016	Environmental data from Niskin bottle sampling during the Fall 2016 ESP deployment in Monterey Bay, CA
Normalized oxygen from MCH incubations	Normalized oxygen from MCH incubations
Normalized oxygen from MCP incubations	Normalized oxygen from MCP incubations
Nutrient and cell count data for incubations	Nutrient and cell count data for incubations
Nutrients and Flow Cytometry 2017	Nutrients and Flow Cytometry 2017
OUTPACE - flow cytometry cell counts	Flow cytometry cell counts obtained during the OUTPACE cruise
Phenotypic plasticity of the ciliated band of early echinoderm larvae	Phenotypic plasticity of the ciliated band
Phosphohydrolysis rates in the coastal western North Atlantic	Phosphohydrolysis rates in the coastal western North Atlantic
Phosphorus uptake - biogeochemistry	Bulk phosphorus uptake by microbes
Phosphorus uptake - kinetics	Phosphorus uptake kinetics by microbes to whole communities
Phosphorus uptake - taxon	Bulk phosphorus uptake by microbes
Photosynthesis and Respiration	Photosynthesis and Respiration
Physiological Response Metrics	Physiological Response Metrics
Physiology of Montipora capitata and Porites compressa in response to marine heatwaves
Phyto Growth and Grazing FCM IN2019_V03	Phytoplankton growth and grazing from flow cytometry in the eastern Indian Ocean
phytoplankton	Phytoplankton (Prochlorococcus, Synechococcus, and PEuks) by flow cytometry.
Phytoplankton Hetertrophic Bacteria Cell Abundance
Phytoplankton phosphonate utilization
phyto_abund_NAtl	phytoplankton abundances from temperature/pCO2 experiments on North Atlantic microzooplankton
phyto_temp_iron_Ant1	Phytoplankton-related data from temp and iron experiments on Antarctic phyto and microzooplankton
Pico- and nanoplankton abundance: FCM: EN532 and EN538	Pico- and Nanoplankton concentrations from ctd cast deployments on Endeavor 532 and Endeavor 538
Pico-cyanobacteria concentration and cell properties	Diel series of pico-cyanobacteria concentration and cell properties.
Picocyanobacteria and picoeukaryote growth under different HOOH concentrations	Picocyanobacteria and picoeukaryote growth under different HOOH concentrations
picophyto_FCM_lab	Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis).
picophyto_FCM_ship	Picophytoplankton abundances of live samples determined by flow cytometry (shipboard analysis).
Picoplankton	Picoplankton Abundance Data
Plankton size spectra	Plankton size spectra from 5 projects: CCE LTER, HOT, CRD FluZIE, BLOOFINZ-GoM, and SalpPOOP from 2004-2018
Pleurochrysis carterae growth	Growth rate of Pleurochrysis carterae CCMP645 cells as measured by flow cytometry
Prochlorococcus and Synechococcus nitrite accumulation in batch culture	Prochlorococcus and Synechococcus nitrite accumulation in batch culture
Prochlorococcus in situ cell cycle phases	Prochlorococcus in situ Cell Cycle Phases Fractions.
Prochlorococcus N and C uptake	Prochlorococcus N and C uptake
Prochlorococcus_narB	Abundance of Prochlorococcus cells containing gene narB at HOT and BATS
Profile data
PRS bacteria identification	Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1
Quantification of ciliated band length per unit protein in early echinoderm larvae: biometirc data	Ciliated band length of early echinoderm larvae
Quantification of ciliated band length per unit protein in early echinoderm larvae: protein data	Protein content of early echinoderm larvae
Red Sea Gradient Physiology	Red Sea Gradient Physiology
Reef biogeochemistry, metabolomics, and metagenomics	Reef biogeochemistry, metabolomics, and metagenomics
Reef seawater biogeochemistry from the Jardines de la Reina reef-system
Ruegeria pomeroyi OD and FCM
RV Atlantic Explorer BATS BV50 Plankton cell abundances and APA	Plankton cell abundances and APA data.
RV Atlantic Explorer BATS BV50: CO2 and PO4	CO2 fixation and PO4 uptake rates
Sargasso Sea Hydrocarbons
SBDOM11 mini-remineralization expt.	bacterial abundance, growth rates, and DOC from SBDOM11 cruise
Seawater nutrient/metabolite and flow cytometry metadata - Picoplankton incubation experiment 2020
Series 1B: cell size and abundance	Series 1B: cell size, abundance
Series 2A: cell size and abundance	Series 2A: cell size, abundance
Series 3A: cell abundance	Series 3A: cell abundance
Series 3A: cell size	Series 3A: cell size
Series 3B: bubble expt flow cytometry	Series 3B: bubble expt flow cytometry
Series 3B: bubble expt LC computed	Series 3B: bubble expt LC computed photochemical measurements
Series 3B: bubble expt LC raw	Series 3B: bubble expt LC raw
Series 3B: bubble expt NPQ computed	Series 3B: bubble expt NPQ computed photochemical measurements
Series 3B: bubble expt NPQ raw	Series 3B: bubble expt NPQ raw
Series 4A: cell abundance and size	Series 4A: cell abundance and size
SMILE counts	SMILE counts
SoMMoS dataset	Southeastern Mediterranean Monthly cruise Series
Sorted Cell Quotas	Sorted Cell Quotas
SPOT Cell Counts	Microbial cell counts in seawater samples from SPOT cruises in 2017
Switch 2014 Metadata	Cell abundance, nutrient, DMSP concentrations from mesocosm experiment
Syn-Pro Co-cultures	Syn-Pro Co-cultures
Synechococcus Batch Culture Sampling Data
Synechococcus growth and genetic sequence accessions from pCO2 experiments
Temperature	Temperature
The Long Term Phytoplankton Evolution Experiment: Culture Analysis	The Long Term Phytoplankton Evolution Experiment: Culture Analysis
The Long Term Phytoplankton Evolution Experiment: Genomic Analysis	The Long Term Phytoplankton Evolution Experiment: Genomic Analysis
Tidal study of seawater microbial communities
TORCH-II	Coastal water biogeochemistry along the North Atlantic coast
USVI Time-series	USVI Time-series
Virus production at a hydrothermal vent site
Vitacopss: cell counts and chla	Picoplankton growth with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015
Vitacopss: nutrients and microbial cell counts	Nutrients and picoplankton cell counts with nitrate and B vitamins from San Pedro Ocean Timeseries (SPOT), 2015
water nutrients	Florida Keys - Water Nutrients
water_samples	Depth profiles of oceanographic parameters from Chesapeake Bay in Spring and Summer of 2010-2011

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Instrument: Flow Cytometer