Instrument: Spectrophotometer

Acronym: Spectrophotometer
External Identifier: skos:broadMatch: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/

Description

An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.


Dataset NamePI-Supplied DescriptionPI-Supplied Name
16S rRNA sequence data from venting fluids and microbial mats; collected on R/V Atlantis cruise AT18-08 at the Axial Seamount, Juan de Fuca Ridge in 2011Turner Biosystems spectrophotometer
Data from two Antarctic krill (Euphausia superba) 24-hour feeding experiments under ambient temperature and pCO2, ambient temperature and elevated pCO2, and elevated temperature and pCO2.Used to measure pH in seawater. Shimadzu spectrophotometer
Growth of Antarctic krill (Euphausia superba) from a 1-month experiment at ambient conditions, ambient temperature and elevated pCO2, or elevated temperature and elevated pCO2, Jan-Feb. 2014Used to measure pH in seawater. Shimadzu spectrophotometer
Growth of Antarctic krill (Euphausia superba) from a 1-month experiment at ambient conditions, ambient temperature and elevated pCO2, or elevated temperature and elevated pCO2, Jan-Feb. 2015Used to measure pH in seawater. Shimadzu spectrophotometer
Growth of Antarctic krill (Euphausia superba) from an experiment at two temperatures and three pH levels, Feb-Mar. 2015Used to measure pH in seawater. Shimadzu spectrophotometer
DIC, TA, pH, DO from nGOM cruises conducted aboard the R/V Pelican throughout 2017, 2018 and July 2019.Winkler titration was used for DO_spec analysis. DO_spec samples were measured spectrophotometrically using Genesis 30 (Thomas Scientific) spectrophotometer at 466 nm.
Estimated feeding rates of adult and nauplius copepod species on three phytoplankton species from the San Francisco estuary in 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Experimental results describing copepod gut evacuation time from species collected in the San Francisco estuary during 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Experimental results describing the minimum time for copepod gut fluorescence by plate reader method analyzed at San Francisco State University during 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Experimental results describing if sonication is needed for chlorophyll extraction by copepods in the San Francisco estuary conducted during 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Experimental results describing Pseudodiaptomus marinus gut minimum fluorescence thresholds that were analyzed at San Francisco State University in 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Water column phosphate data from RV/Atlantic Explorer AE1812, May 2018Used to measure total particulate phosphate concentrations spectrophotometer (Thermo)
Sub-seafloor metatranscriptomes from anaerobic Peru Margin sediments collected on R/V JOIDES Resolution Leg 201 in 2002We followed the manufacturer’s instructions for cDNA amplification, and the resulting quantity of cDNA was checked on a Nanodrop (Thermo Scientific) and Fluorometer (Qubit 2.0, Life Technologies). Nanodrop
AQMET Air quality and meteorological measurements measured measured during DANCE cruise HRS1414 aboard the R/V Hugh R. Sharp from July to August 2014.Aerodyne CAPS
Phosphorus redox and water column phosphorus data from R/V Neil Armstrong AR16 in the Western North Atlantic from 2017-05-04 to 2017-05-20As with the SRP samples, the TPP samples were analyzed via the molybdenum blue method using a spectrophotometer (Thermo).  spectrophotometer (Thermo)
Sediment geochemistry summary from push cores collected during HOV Alvin dives during the R/V Atlantis cruise AT37-06 in the Guaymas Basin from December 2016Hach Company DR 2800 Spectrophotometer
Sediment geochemistry summary from push cores collected during HOV Alvin dives during the R/V Atlantis cruise AT37-06 in the Guaymas Basin from December 2016Shimadzu Instruments UV1601 Spectrophotometer
Sediment geochemistry from push cores collected during HOV Alvin dives during the R/V Atlantis cruise AT42-05 in Guaymas Basin, Gulf of California (27 00.00 N, -111 20.00 W) in November 2018Hach Company DR 2800 Spectrophotometer
Sediment geochemistry from push cores collected during HOV Alvin dives during the R/V Atlantis cruise AT42-05 in Guaymas Basin, Gulf of California (27 00.00 N, -111 20.00 W) in November 2018Shimadzu Instruments UV1601 Spectrophotometer
Geochemical measurements of CTD and Alvin Niskin samples collected in the Gulf of California on RV/Atlantis AT42-05 Alvin dives, Nov. 2018Shimadzu Instruments UV1601 Spectrophotometer
Geochemical measurements of CTD and Alvin Niskin samples collected in the Gulf of California on RV/Atlantis AT42-05 Alvin dives, Nov. 2018Hach Company DR 2800 Spectrophotometer
ATP and cell numbers in laboratory experiments with Thalassiosira weissflogii conducted in 2018Shimadzu UV-2401PC
ATP and cell numbers in laboratory experiments with Thalassiosira weissflogii conducted in 2019Shimadzu UV-2401PC
Results from laboratory experiments testing autoinduction in marine diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum (PhytoplanktonQS project)Accurate to 4 decimal places Cary-100 UV-Visible spectrophotometer
Iodine speciation and isotope ratio values from iodine radiotracer incubation experiments conducted on the R/V Atlantic Explorer cruise AE1825 with samples collected at BATS and Hydrostation S in September of 2018All iodate concentrations were measured via spectrophotometery on a VWR UV-Vis Scanning 3100 PC spectrophotometer and accompanying UV-Vis Analyst software via a method outlined by Jickells (1988). VWR UV-Vis Scanning 3100 PC spectrophotometer
Iodine speciation and superoxide concentration depth profile value from iodine radiotracer incubation experiments conducted on the R/V Atlantic Explorer cruise AE1825 with samples collected at BATS and Hydrostation S in September of 2018All iodate concentrations were measured via spectrophotometery on a VWR UV-Vis Scanning 3100 PC spectrophotometer and accompanying UV-Vis Analyst software via a method outlined by Jickells (1988). VWR UV-Vis Scanning 3100 PC
Benthic flux data from sediment cores collected in the York River Estuary, VA in 2020Extracted chlorophyll-a was analyzed on a Beckman Coulter DU800 Spectrophotometer. Beckman Coulter DU800 Spectrophotometer
Proximate biochemistry of sponge species collected in 2017 and 2018 across the Caribbean Basin in Curacao, Belize, Grand Cayman, St. CroixEppendorf Biophotometer
Biogenic and lithogenic silica concentrations from marine suspended particles collected during the 2011 CLIVAR S04P expedition on RVIB Nathaniel B. Palmer from February 19 to April 19, 2011Spectrophotometer
Biogenic Silica from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022Downstream analysis was done using a modified sodium hydroxide digestion method as described in Krause et al. 2009. Micro-molar of silicon was calculated from transmission using a spectrophotometer with a 1 cm cuvette. spectrophotometer
Biogeochemistry and microbiology from the R/V Hermano Gines cruises in the Cariaco Basin from 1995 to 2015 (CARIACO Ocean Time-Series Program)Upon return to the laboratory, the ZnS is dissolved and is analyzed spectrophotometrically by the method of Cline (1969). spectrophotometrically
Bottle chemistry data from hydrothermal vents sampled by CTD rosette during R/V Atlantis cruise AT37-12 in April and May 2017Ocean Optics QEPro
Bottle chemistry data from hydrothermal vents sampled by CTD rosette during R/V Atlantis cruise AT37-12 in April and May 2017Agilent 8454
Bottle data and chemical analysis from Falkor cruise FK180624 in the Eastern Tropical North Pacific Ocean in 2018NO2, PO4: Ocean Optics QEPRO spectrophotometer with 10cm Starna flow through quartz cell Ocean Optics QEPRO spectrophotometer
Bottle data and chemical analysis from Falkor cruise FK180624 in the Eastern Tropical North Pacific Ocean in 2018pH: Agilent 8454 spectrophotometer with custom front end for automated syringe injection Agilent 8454 spectrophotometer
Brooded coral larval respiration (Cumbo, 2012) from Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)USB2000, Ocean Optics spectrophotometer
Respiration rates, photosynthetic efficiency, and mortality of brooded coral larval experiments, March 2011 and 2012, Taiwan (Cumbo, 2013) (MCR LTER project, Climate_Coral_Larvae project)USB2000, Ocean Optics spectrophotometer
Protein content of brooded coral larvae at high and ambient temperature and pCO2, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)- USB2000, Ocean Optics - plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA) spectrophotometer
Respiration and protein content of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)- USB2000, Ocean Optics - plate reading spectrophotometer (Biotek Synergy H4 Hybrid Reader, USA) spectrophotometer
Respiration of brooded coral larvae at high and ambient temperature and pCO2, Taiwan, March 2011 (Cumbo et al, JEMBE, 2013) (MCR LTER & Climate Coral Larvae projects)USB2000, Ocean Optics spectrophotometer
Porites coral calcification responses to declining Ωar in a CO2 manipulation experiment in Palau versus the calcification responses observed in ten other studies of massive Porites coralsSpectrophotometric pH measurements were made with 2 mM m-Cresol purple indicator dye using a spectrometer with a 100 mm flow cell (Ocean Optics, mean precision = 0.005). Ocean Optics pH spectrophotometer
Calcification rates of Porites corals collected from a naturally high-Ωar reef and a naturally low-Ωar reef in Palau incubated at three experimental Ωar conditionsSpectrophotometric pH measurements were made with 2 mM m-Cresol purple indicator dye using a spectrometer with a 100 mm flow cell (Ocean Optics, mean precision = 0.005). Ocean Optics pH spectrophotometer
Carbonate chemistry associated with copepod experiments with varying CO2 (OA-Copepod_PreyQual project)To measure pH
P17N Ocean Acidification Pteropod Study: Carbonate chemistry system and hydrography samples from R/V New Horizon NH1208 from a transect 35-50 degrees North, Pacific Ocean, Aug-Sep 2012 (OAPS project)Agilent 8453 UV-VIS spectrophotometer: measures the relative absorption of pH-sensitive indicator at different wavelengths.
Carbonate chemistry in Mission Aransas Estuary from May 2014 to Feb 2017 and Dec 2018 to Feb 2020Agilent 8453 UV-Vis spectrophotometer
Carbonate chemistry and CTD data collected along a North Pacific transect between Hawaii and Alaska on R/V Kilo Moana cruise KM1712 in August 2017Varian Cary 400 spectrophotometer
Carbonate chemistry effects from Hurricane Harvey in San Antonio Bay and Mission Aransas Estuary from 2017-02-22 to 2018-11-15pH - 8453 UV-Vis spectrophotometer, Agilent  8453 UV-Vis spectrophotometer
Elemental and isoptopic composition of sediment and biomass from the Carpinteria Salt March sampled in 2020 and 2021 Genesys 150 UV-Vis Spectrophotometer to measure iron extracts Genesys 150 UV-Vis Spectrophotometer
Chromophoric dissolved organic matter (CDOM) from two microcosm incubation experiments conducted under three light treatments using water originating from West Bay of the Neuse River Estuary, North Carolina USA in 2021 and 2022Genesys 10S UV-Vis Spectrophotometer (ThermoFisher): used in scanning mode, fast, at 1 nm intervals to measure the absorbance of the filtrate. Genesys 10S UV-Vis Spectrophotometer
An investigation into the characteristics of the reactive pools of Mississippi River plume sediments collected August to September of 2016 and May 2017 aboard the R/V Pelican.Genesys 10S UV-Vis Spectrophotometer.
In situ porewater data from the Cocos Ridge (Eastern Equatorial Pacific) acquired during cruise SR2113 between November - December 2021Optical fibers connected channels to a custom tungsten halogen light source and Ocean Optics USB4000 spectrophotometers. Spectrophotometers were housed in a small refrigerator regulated to 18°C.  The spectrophotometers were connected to a portable computer with custom software that monitors and records absorbances and sample information. The encased water bath had a lid with openings for AT sample input, CO2 gas tubing, and two Luer lock ports for pH sample input and output. custom tungsten halogen light source and Ocean Optics USB4000 spectrophotometer
In situ porewater data from the Cocos Ridge (Eastern Equatorial Pacific) acquired during cruise SR2113 between November - December 2021Picarro Cavity Ring-Down Spectrometer (G2131-i) with Liaison autosampler.
Solid phase measurements of sediment cores from the Cocos Ridge (Eastern Equatorial Pacific) acquired during cruise SR2113 between November - December 2021.Elemental Analyzer (EA, Costech) coupled to a Picarro Cavity Ring-Down Spectrometer (G2131-i) was used to measure Total Carbon. A Picarro Cavity Ring-Down Spectrometer (G2131-i) coupled to a Liaison autosampler was used to measure PIC and delta13C of PIC. Picarro Cavity Ring-Down Spectrometer (G2131-i)
Water column data from the Cocos Ridge (Eastern Equatorial Pacific) acquired during cruise SR2113 between November - December 2021The pH of each sample was determined on an Agilent 8453 spectrophotometer setup with a custom-made temperature-controlled cell holder. Only the tungsten lamp was turned on. The UV lamp was turned off to prevent photodegradation of organic matter in the samples by UV light. A custom macro program running on Agilent UV-Visible ChemStation Software Rev. B.04.01 was used to guide the measurements and data processing. The macro automated the procedures of sample input information, blank and sample scans, quality control, and data archiving. The quality control steps included checking the baseline shift after dye injection and monitoring the standard deviation of multiple scans. Absorbance blanks were taken for each sample, and 10 μL of purified m-cresol purple (10 mmol kg-1) was added for the analysis. pHT(total scale) was calculated according to Müller and Rehder (2018).
pH was measured with a spectrophotometer.
Bacterial transcriptional response to picoeukaryote Micromonas commodaMeasurements for ammonium (NH4+) were done separately via the phenate method with spectrophotometric analysis on a Model Spectronic 21D (Spectronic Instrumentation). Model Spectronic 21D (Spectronic Instrumentation)
Coral carbon, oxygen, and boron isotopes; coral Sr/Ca, Mg/Ca, U/Ca, and Ba/Ca; coral chlorophyll-a from samples from reef field sites in Puerto Morelos, Mexico & Kaneohe Bay, HawaiiChlorophyll a was determined using a Shimadzu UV-VIS spectrophotometer and the equations of Jeffrey and Humphrey. Shimadzu UV-VIS spectrophotometer
The effects of nutrient enrichment and predation on coral health and microbiomes from June to August 2013 in Key Largo, FL (small grazers facilitating fungal disease project)To ensure nutrient diffusion tubes were effective in releasing nutrients, we analyzed inorganic nitrogen and phosphorus spectrophotometrically from water samples taken within ~3 cm of 5 control corals and ~1 cm from 5 nutrient diffusion tubes once during the experiment a few days after fertilizer pellets were placed in diffusion tubes (UV-2450, Shimadzu; Southeast Environmental Research Center, Miami, FL). spectrophotometrically
Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio.A Thermo Scientific Genesys spectrophotometer was used to measure the intensity of light transmission at specific wavelengths for chlorophyll a, protein and carbohydrate samples of both species. Thermo Scientific Genesys
Montipora capitata and Porites compressa Physiological Measurements for Experimental Corals and Parent Colonies from 2014-2015 (RAPID Hawaii project)A Thermo Scientific Genesys spectrophotometer was used to measure the intensity of light transmission at specific wavelengths for chlorophyll a. Thermo Scientific Genesys
Protein measurements from coral-light experiments (Dufault, 2013, JEB), Taiwan 2010 (MCR LTER project, Climate_Coral_Larvae project)96-well plate spectrophotometer: Synergy H4 Hybrid Reader, Biotek, Winooski, VT, USA; the assay was calibrated using BSA spectrophotometer
Fluorescence and Physical Indicators for Sediment Cores from a Protected island in south Wilkinson Bay in the northeast portion of Barataria Bay, Louisiana in September 2018Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA)
Fluorescence and Physical Indicators for Sediments Cores from Barataria Basin, Louisiana, for March and November 2017Cary 100 Spectrophotometer (Varian Inc., Palo Alto, CA)
Extrapolated pH measurements of CRMsAgilent 8454 UV-Vis Spectrophotometer
Raw absorbance and R measurements of CRMsAgilent 8454 UV-Vis Spectrophotometer
Biogeochemical data collected from porewater profiles within a shallow, sandy subterranean estuary (STE) in Virginia USA, from 2018 - 2020spectrophotometer
CTD sensor and nutrient measurements from R/V Yellowfin cruises to the San Pedro Ocean Time-series (SPOT) station in 2013 and 2014Silicic acid and phosphate analyses were done colorimetrically at USC with a Hitachi UV/vis-spectrophotometer. Hitachi UV/vis-spectrophotometer
Soluble Mn speciation from CTD casts in the Ross Sea, Southern Ocean taken during RVIB Nathaniel B. Palmer cruise NBP1801 in Jan-Feb 2018The analytical setup uses a 100 cm liquid waveguide capillary cell (World Precision Instruments) coupled with an Ocean Optics UV/Vis spectrophotometer in which a mini deuterium halogen light source (DT-Mini-2-GS) is coupled with a USB2000+ fiber optic spectrometer, controlled with SpectraSuite software.  Ocean Optics UV/Vis spectrophotometer
Experiment with the diatom Chaetoceros sp. on the impact of temperature, light climate, and carbonate chemistry on TEP production and aggregation processes from May 2015 (OA - Effects of High CO2 project)The stained TEP filters were dissolved in sulfuric acid for 4h and measured coloimetrically through absorption at 787nm on a spectrophotometer (Thermo Scientific Genesys 105 VIS). spectrophotometer (Thermo Scientific Genesys 105 VIS)
Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: Detected 15N-labeled ammonium (NH4) in commercial 15N2 gas (15N2 Contamination project)Ammonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer. AJN Scientific f-2500 Fluorescence Spectrophotometer
Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: Detected 15N-labeled nitrate (NO3) in commercial 15N2 gas (15N2 Contamination project)Ammonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer. AJN Scientific f-2500 Fluorescence Spectrophotometer
DEPRECATED: Optical properties of Orbicella faveoalta fragments (chl-a, Symbiodinium density, absorbance, and absorbance efficiency) from Rosaria and Varadero reef sites, Colombia, 2016 and 2017 (Varadero Reef project)mini spectrophotometer (Ocean Optics USB4000)
Dissolved and particulate carbon and nitrogen data from seawater collected during CCGS John P. Tully cruises in the northeast Pacific Ocean from Vancouver Island to Station P from 2018 to 2020Agilent 8453 UV-visible Spectroscopy System (aka Agilent 8453 spectrophotometer) has average precision of ±0.004. Agilent 8453 spectrometer
Discrete lab results from USCGC Healy cruise HLY1202 from the Arctic, North of Alaska in 2012 (OA - Canada Basin project)Samples were transported to the USGS Carbon Chemistry Lab in St. Petersburg, Fla. Total alkalinity samples were analyzed using an Ocean Optics USB 2000 spectrophotometer, bromol cresol purple indicator dye, and the methods of Yao and Byrne (1998). Ocean Optics USB 2000 spectrophotometer Ocean Optics USB 2000 spectrophotometer
DIC, TA, pH from R/V Pelican cruise conducted in the northern Gulf of Mexico in April and July 2017Genesis 30 (Thomas Scientific) spectrophotometer at 466 nm
DOC, TDN, and Nutrients from the RVIB Nathaniel B. Palmer NBP1302 cruise in the Ross Sea during 2013 (TRACERS project)Phosphate was determined colorimetrically according to Grasshoff (1976) using a fiber optic spectrophotometer (Ocean Optics) fiber optic spectrophotometer (Ocean Optics)
Zooplankton community abundances from meter net tows on multiple cruises on RV/Savannah in the South Atlantic Bight, Mid-Continental Shelf from 2015-2017Used for DNA quantitation and quality testing. Thermo Scientific™ NanoDrop Lite Spectrophotometer
Seawater chemistry treatment conditionspH was measured with a spectrophotometer
Chemical analysis from sediment core pore water samples collected in the back barrier island bays on Virginia’s Eastern Shore in July 2017Used to measure total dissolved sulfide and t​otal dissolved iron Ocean Optics USB400 UV-Vis spectrophotometer
Sediment core sample metadata collected July 2017 in the back barrier island bays on Virginia’s Eastern ShoreUsed to measure total dissolved sulfide and t​otal dissolved iron. Ocean Optics USB400 UV-Vis spectrophotometer
Population changes in Halobacteriovorax cultured with protist & preyNanoDrop ND-1000 UV spectrophotometer (Thermo Scientific, Wilmington, USA) NanoDrop ND-1000 UV spectrophotometer
Carbonate data in experimental treatments of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Experimental results: Chlorophyll-a, POC, and cell volume of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Carbon and nitrogen content of E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Experimental results: Emiliania huxleyi growth rates under different pCO2 levels from Bellingham, WA from 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Experimental results: cellular particulate DMSP from E. huxleyi at 3 pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Experimental results: Emiliania huxleyi growth rates under different pCO2 levels, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
SEM based morphology of Emiliania huxleyi, 2011-2012 (E Hux Response to pCO2 project)Agilent 5480 UV-VIS spectrophotometer (+/- 0.02) spectrophotometer
Measurements of Chlorophyll, NO2, NO3, PO4, Silicate, NH4, PIC, POC, PON, BSi from CTD casts on R/V Endeavor cruise EN616 in July 2018Hitachi U-3010 spectrophotometer (SN 0947-010)
Fe(II) concentrations over time in iron oxidizing bacteria cultures.Used to measure ferrozine assay Cary 100 UV-Vis Spectrophotometer (Agilent Technologies)
Iron concentrations of phage from experiments of iron-labelled E. coli infected with T4 and T5 bacteriophage, 2018 and 2019.Used to measure bacterial cell concentrations.
Oxygen consumption rates/zero valen iron dissolution of FeOB (Ferrozine assay) with Kanamycin additionUsed to measure ferrozine assay Cary 100 UV-Vis Spectrophotometer (Agilent Technologies)
Field physiochemical parameters including nutrient concentrations and nitrogen specific uptake rates from samples collected between 2017 and 2019 from the Arctic Ocean, California Coastal Current, and a Chesapeake Bay estuaryUsed to measure concentrations of dissolved primary amines Shimadzu RF-600 Spectrofluorophotometer
HPLC data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic, offshore from North Carolina, USA, in 2021 and 2022High performance liquid chromatography (HLPC) coupled to an in-line photodiode array spectrophotometer (Jeffrey et al.  1997).
Hydrologic data from field sampling sites in the Neuse River Estuary, Pamlico Sound, and Onslow Bay in the coastal North Atlantic, offshore from North Carolina, USA, in 2021 and 2022Shimadzu UV-160U spectrophotometer
Experimental results describing Copepod fed phytoplankton mixtures that were analyzed at San Francisco State University in 2013Agilent 8453 spectrophotometer (Agilent Technologies) Spectrophotometer
Geochemical measurements of porewater from sediment push core samples in the Gulf of California during R/V Falkor cruise FK190211 in 2019Hach Company DR 2800 Spectrophotometer
Geochemical measurements of porewater from sediment push core samples in the Gulf of California during R/V Falkor cruise FK190211 in 2019Shimadzu Instruments UV1601 Spectrophotometer
Geochemical measurements of CTD and Alvin Niskin samples collected in the Gulf of California during R/V Falkor cruise FK190211 in 2019Hach Company DR 2800 Spectrophotometer
Geochemical measurements of CTD and Alvin Niskin samples collected in the Gulf of California during R/V Falkor cruise FK190211 in 2019Shimadzu Instruments UV1601 Spectrophotometer
Geochemistry and microscopy from incubation experiments of Chesapeake Bay sediments conducted at Horn Point LaboratoryUsed to determine NH4 and Fe ThermoScientific Genesis UV/Vis
Summary geochemistry and radiotracer rates for 30 marine sediment cores covering eight sites and four geochemical regimesShimadzu Instruments Spectrophotometer Model: UV‐1601; Calibrated with laboratory made standards in envornmentally relevant concentration ranges and spectrum tuned for method. KH2PO4 for phosphate (0-40 uM in MQ; 885 nm wavelength); Na2S for sulfide (1-3 uM in MQ; 670 nm wavelength); NH4Cl for ammonium (0-60 uM; 640 nm wavelength) Shimadzu UV-1601
POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line I07N RB1803 in the Western Indian Ocean from April to June 2018 (Ocean Stoichiometry Project)After allowing the mixed reagent to react for exactly 30 min, the standards and samples were analyzed in 30-second intervals in a spectrophotometer at an 885 nm wavelength using a blank of ~0.1 M HCl solution and rinsing the cuvette with the blank solution between measurements. Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
Water column GeoFish and bottle pH data from Leg 1 (Seattle, WA to Hilo, HI) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1814) on R/V Roger Revelle from September to October 2018automated Ocean Optics UV-VIS spectrophotometric system
Water column GeoFish and bottle pH data from Leg 2 (Hilo, HI to Papeete, French Polynesia) of the US GEOTRACES Pacific Meridional Transect (PMT) cruise (GP15, RR1815) on R/V Roger Revelle from October to November 2018automated Ocean Optics UV-VIS spectrophotometric system
Size-fractionated major and minor particle composition and concentration collected from RV Thompson (TN303) along the US GEOTRACES EPZT transect in the Eastern Tropical Pacific during 2013 (US GEOTRACES EPZT project)Used to run samples manually Shimadzu UV-2550 UV-VIS Spectrophotometer
Arsenate, iodide, and selenium concentrations collected from Super-GeoFish during the R/V Thomas G. Thompson cruise TN303 from Peru to Tahiti in 2013 (U.S. GEOTRACES EPZT project)selective hydride generation/atomic absorption spectrometry
Arsenate, iodide, and selenium concentrations collected from Go-FLO bottles during the R/V Thomas G. Thompson cruise TN303 from Peru to Tahiti in 2013 (U.S. GEOTRACES EPZT project)selective hydride generation/atomic absorption spectrometry
Carbonate chemistry data for low-high pCO2 acclimated E. huxleyi cultures (E Hux Response to pCO2 project)Agilent 8453A UV-VIS Diode Array Spectrophotometer
Grazing experiment 4: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Grazing experiment 4: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Agilent 8453A UV-VIS Diode Array Spectrophotometer
Grazing experiment 5: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Grazing experiment 6: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Grazing experiment 6: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Grazing experiment 7: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Grazing experiment 7: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)Spec20D+ spectrophotometer
Metagenomic, metatranscriptomics and 16S rRNA gene sequence data from diel sampling at Groves Creek Marsh, Skidaway Island, GA during July 2014Filtered samples (non-acidified) were placed in a 1 cm quartz absorbance cell situated in the light path of an Agilent 8453 ultraviolet-visible spectrophotometer and CDOM absorbance spectra were recorded from 190 to 800 nm. Agilent 8453 ultraviolet-visible spectrophotometer
CDOM and FDOM full-depth Nisken profiles from high-resolution surveys of DOM optical properties conducted during the R/V Melville cruise MV1310 in the Gulf of Alaska (August 4–21, 2013)CDOM: Single-beam liquid waveguide capillary flow cell (World Precision Instruments Inc., measured at 92.2 cm pathlength), coupled with quartz fiber optic cables to a deuterium-tungsten light source (DT-mini GS) and a MAYA2000 PRO spectrophotometer (both from Ocean Optics, Inc.). MAYA2000 PRO spectrophotometer
CDOM and FDOM full-depth Nisken profiles from high-resolution surveys of DOM optical properties conducted during the R/V Melville cruise MV1310 in the Gulf of Alaska (August 4–21, 2013)CDOM: Single-beam liquid waveguide capillary flow cell (World Precision Instruments Inc., measured at 92.2 cm pathlength), coupled with quartz fiber optic cables to a deuterium-tungsten light source (DT-mini GS) and a MAYA2000 PRO spectrophotometer (both from Ocean Optics, Inc.). MAYA2000 PRO spectrophotometer (Ocean Optics, Inc.)
Gulf of Finland chromophoric dissolved organic matter (CDOM) Naperian absorption coefficientsA Shimadzu UV-2501PC UV-VIS recording spectrophotometer with UV-Probe software and Agilent 8453 UV-visible spectrophotometer with ChemStation software were used to take chromophoric dissolved organic matter absorbance measurements. Shimadzu UV-2501PC UV-VIS recording spectrophotometer
Chromophoric Dissolved Organic Matter (CDOM) Naperian Absorption Coefficients from the Gulf of Finland from June to July 2019 (MODIE project)Shimadzu UV-2501PC UV-VIS recording spectrophotometer with UV-Probe software and Agilent 8453 UV-visible spectrophotometer with ChemStation software: chromophoric dissolved organic matter absorbance measurements. At the Tvärminne Zoological Station, a Shimadzu UV-2501PC UV-VIS recording spectrophotometer with UV-Probe software was used, with air as an internal reference and Milli-Q water as blanks. Absorbance (A(λ)) in the control and irradiated samples were measured at 250–800 nm at 1.0 nm intervals, in duplicate, in a 1-cm-pathlength quartz spectrophotometric cuvette. Shimadzu UV-2501PC UV-VIS Recording Spectrophotometer
Chromophoric Dissolved Organic Matter (CDOM) Naperian Absorption Coefficients from the Gulf of Finland from June to July 2019 (MODIE project)At Skidaway Institute of Oceanography, absorbances were measured using an Agilent 8453 UV-visible spectrophotometer with ChemStation software, with the same parameters as above and Milli-Q water as blanks. Absorbance (A(λ)) in the control and irradiated samples were measured at 250–800 nm at 1.0 nm intervals, in duplicate, in a 1-cm-pathlength quartz spectrophotometric cuvette. Agilent 8453 UV-visible spectrophotometer
Chemical analysis from sediment core pore water samples collected in the northern Gulf of Mexico, May 2017Used to measure total dissolved sulfide and total dissolved iron. Ocean Optics USB400 UV-Vis spectrophotometer
Sediment core sample metadata collected in the northern Gulf of Mexico, May 2017Used to measure total dissolved sulfide and t​otal dissolved iron. Ocean Optics USB400 UV-Vis spectrophotometer
Fluorescence and oxygen from Hood Canal, Puget Sound, Washington, USA from R/V Clifford A. Barnes CB960, CB974, CB980, CB985 from 2011-2012 (Nitrification and Marine Planktonic Biodiversity project)spectrophotometer
Nutrient concentrations from Hood Canal, Puget Sound, Washington, USA from R/V Clifford A. Barnes CB960, CB974, CB980, CB985 from 2011-2012 (Nitrification and Marine Planktonic Biodiversity project)spectrophotometer
Host-symbiont respiration related to symbiont density; anemones from Key Largo from (AnemoneOA project)ThermoFisher, Waltham, MA, USA NanoDrop spectrophotometer
Niskin bottle water samples and associated CTD measurements from the Hawaii Ocean Time-Series cruises from 1988-2023Used for spectrophotometric seawater pH measurements
Sediment trap flux measurements for the Hawaii Ocean Time-Series (HOT) project from December 1988 to December 2023 at Station ALOHAspectrophotometer (Perkin-Elmer Lambda 3B) and 1-cm cuvette spectrophotometer and 1-cm cuvette
pH was analyzed using the spectrophotometric method and purified m-cresol purple (mCP) obtained from Dr. Robert Byrne's lab (University of South Florida), and was analyzed on the total scale with a precision of +/-0.0004. Prior to analyses, a calibrated OrionRoss glass electrode was used to adjust the indicator to pH 7.92+/-0.01, and a 10 cm water-jacketed absorbance cell of pH was kept at 25+/-0.01 deg.C. Spectrophotometric Method
pH was analyzed using the spectrophotometric method and purified m-cresol purple (mCP) obtained from Dr. Robert Byrne's lab (University of South Florida), analyzed on the total scale with a precision of +/-0.0004. Prior to analyses, a calibrated OrionRoss glass electrode was used to adjust the indicator to pH 7.92+/-0.01, and a 10 cm water-jacketed absorbance cell of pH was kept at 25+/-0.01 degrees C. Spectrophotometric method
Results of 18S sequencing of full-length 18S rDNA for metabarcoding samples collected during R/V Point Sur cruise PS18-09 in the Western Gulf of Mexico in September 2017DNA concentration and quality were evaluated using a Nanodrop spectrophotometer (Thermo Fisher Scientific Inc, USA). Nanodrop spectrophotometer
Metabarcoding samples collected from surface and chlorophyll maximum depths from R/V Pt. Sur PS 18-09 Legs 01 and 03, Hurricane Harvey RAPID Response cruise (western Gulf of Mexico) September-October 2017.Used for DNA concentration and quality evaluation. Nanodrop spectrophotometer (Thermo Fisher Scientific Inc, USA)
Radiocarbon DIC, DIC concentration, pH, and [CH4] in Hudson Canyon, northern US Atlantic Margin collected from R/V Endeavor cruise EN541 in July 2014Agilent Cary 100 UV-Visible spectrophotometer
Water geochemistry from surface samples collected along the Neuse River, Neuse River Estuary, and Pamlico Sound in North Carolina between October and December 2016, following the passage of Hurricane MatthewDOM absorbance. Instrument checks performed regularly. Cary UV 300 Spectrophotometer
Porewater sulfate and sulfide concentrations in Alvin pushcore samples collected from Guaymas Basin hydrothermal vents during R/V Atlantis cruise AT42-05 in November 2018 and analyzed at UNC Chapel HillSulfide was quantified spectrophotometrically at UNC-Chapel Hill.
Nutrient and nitrate isotope data from the southern Benguela upwelling system from February to August 2017Phosphate and nitrite concentrations were measured using a Thermo Scientific Genesis 30 Visible spectrophotometer in a configuration with a detection limit of 0.05 µM. Thermo Scientific Genesis 30 Visible spectrophotometer
Incubation experiment data from R/V Melville, R/V Roger Revelle cruises MV1101, RR1202 in the Southern Ocean (30-60S); 2011-2012 (Great Calcite Belt project)Biogenic silica samples (200 mL) were filtered through 0.4 µm 25-mm PCTE filters and then dried at 60°C prior to storage. Samples were digested in 0.2 M NaOH and quantified spectrophotometrically. Spectorphotometer
Proteins identified from the black smoker chimney Inferno hydrothermal vent plume meta-proteome - replicate Av1 - on the Axial seamount off the coast of Washington in 2011. Measured peptide bond absorbance at wavelength 205nm Thermo Scientific Nanodrop 2000/2000c Spectrophotometer
Proteins from Inferno hydrothermal vent plume meta-proteome - replicate Av2Measured peptide bond absorbance at wavelength 205nm Thermo Scientific Nanodrop 2000/2000c Spectrophotometer
Phytoplankton diversity along with carbon and nitrogen uptake rates collected along the GO-SHIP IO9 repeat hydrography section of the Indian Ocean from R/V Roger Revelle cruise RR1604 from March to April 2016Urea and total dissolved phosphorus (for calculation of dissolved organic phosphorus) were determined on a Thermo Scientific Genesys 10UV spectrophotometer. Thermo Scientific Genesys 10UV spectrophotometer
Isotope data from USCGC Healy cruise HLY1202 from the Arctic, North of Alaska in 2012 (OA - Canada Basin project)Analyses were completed at the University of South Florida  Department of Geology Stable Isotope Laboratory using a Thermo-Finnigan Delta V 3 kiloelectron (keV) Isotope Ratio Mass Spectrometer coupled to a GasBench II preparation  device Thermo-Finnigan Delta V 3 kiloelectron (keV) Isotope Ratio Mass Spectrometer
SSU rRNA gene sequences from marine sediments, marine subseafloor, and deep seawater sampled from the Juan de Fuca Ridge Flank from various R/V Atlantis cruises from 2008-2011 (microJdFR project)NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA) was used to quantify resulting genomic DNA. NanoDrop ND-1000 spectrophotometer
Metegenomic sequences from Lau Basin low temperature vents and Loihi Seamount; from R/V Roger Revelle and R/V Thomas G. Thompson cruises RR1211 and TN225 in the Northeast Lau Basin, Loihi Seamount from 2008-2012Nanodrop ND-1000 spectrophotometer
Chlorophyll and pheopigments from filtered water samples from R/V Savannah cruises in the South Atlantic Bight (SAB) continental shelf off Long Bay, January-April 2012 (Long Bay Wintertime Bloom project)Cary Model 3 spectrophotometer
Primary results from the design and validation of an LED-based photochemical reactorPerkin Elmer 650 spectrophotometer
POM concentrations for carbon, nitrogen, and phosphorus from GO-SHIP Line C13.5/A13.5 in 2020Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
POM concentrations for carbon, nitrogen, phosphorus, and chemical oxygen from GO-SHIP Line P18 Legs 1 and 2 in 2016 and 2017Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)
Macrophytes' amelioration of seawater acidity: Water residence time and irradiance of Saccharina latissima from the Gulf of Maine in November 2018The Agilent Cary Diode Array Spectrophotometer 8454 measures a full spectrum, in as little as 0.1 seconds. Agilent Cary 8454
Elemental composition of phytoplankton communities from multivariate mesocosm experiments conducted with a natural phytoplankton community from Narragansett Bay, RI.Turner SP-830 spectrophotometer (Barnstead International) Turner SP-830 spectrophotometer
OTU molecular abundances for coral Symbiodinium, Belize Mesoamerican Barrier Reef System (MBRS), 2014-2015Used to confirm presence of extracted DNA on agarose gel. Nanodrop 2000 Spectrophotometer (Thermo Scientific)
Transparent exopolymer particle (TEP) measurements from the MesoHux mesocosm experiment held in May 2017For extraction, filters were immersed in sulfuric acid (80%) for 2 h and their absorbance was then measured spectrophotometrically (Agilent 8453 UV-visible spectrophotometer) at fixed wavelength of 787 nm. Aglient 8453 UV-visible spectrophotometer
Microscopy counts (Beggiatoa-like filaments, Cable bacteria), together with in situ temperature and salinity and surface sediment chlorophyll concentrations, of ex situ sediment cores collected in the Chesapeake Bay during 2017-2018ThermoScientific Evolution 60S
Phosphate Time Series Data Obtained from an Autonomous Programmable Flow Injection (pFI) Analyzer at CeNVOOS Moss Landing Shore Station during February 2022 (pFI-SI-LOV project)Samples were analyzed autonomously at the station using a pFI analyzer (miniSIA2, GlobalFIA, USA). This instrument was furnished with two High Flow milliGAT pumps with stainless steel heads, a plexiglass 8-port Lab-On-Valve, a 20 cm Long Path Linear Flow cell equipped with a Tungsten-Halogen lamp and a VIS-NIR Flame spectrophotometer (Ocean Insight, USA). The instrument was controlled and the data processed using FloZF software (v 5.2).  VIS-NIR Flame spectrophotometer (Ocean Insight, USA)
Organic carbon, carbonate, and opal data from Multitracers sediment trap samplesAtomic Adsorption Spectrophotometry
Results from experiment examining 15N-labeled contaminants in commercial 15N2 gas: The measured quantity of 15N2 added to nitrate and ammonium solutions equilibrated with 15N2 gasAmmonium concentrations were measured by derivatization with orthophthaldialdehyde (OPA) and fluorometric detection on an AJN Scientific f-2500 Fluorescence Spectrophotometer. AJN Scientific f-2500 Fluorescence Spectrophotometer
Carbon, biogenic Silica, and mass fluxes from Neutrally Buoyant Sediment Trap (NBST) deployments in the North Pacific on R/V Falkor FK170124 in Jan-Feb 2017
Biological, chemical, and physical water quality indicators of the Neuse River, North Carolina from 2008 through 2013Diagnostic phytoplankton photopigments were identified, separated and quantified by high performance liquid chromatography coupled to an in-line photodiode array spectrophotometer (Jeffrey et al.  1997) in-line photodiode array spectrophotometer
Benthic chamber geochemical data obtained with an in situ benthic lander from the R/V Savannah at sampling stations across the Louisiana shelf and slope in the Northern Gulf of Mexico during four cruise between July 2021 and July 2022All spectrophotometric measurements were conducted with a Beckman Coulter DU 720 UV-vis spectrophotometer. Beckman Coulter DU 720 UV-vis
Nitrogen isotope fractionation for ammonium assimilation by marine phytoplankton (Biological Nitrogen Isotope Fractionation project)Used to measure NH4+ concentration < 50 µM. U-3010 VIS Spectrophotometer
Chromophoric Dissolved Organic Matter Napierian Absorption Coefficients for Open Ocean Blue Water Samples from the North Atlantic Subtropical Gyre from R/V Atlantic Explorer AE1707, AE1829, AE1820, AE1905 at Bermuda Atlantic Time Series Stations (BATS) fChromophoric dissolved organic matter absorbance measurements were made from samples taken from July 17, 2018 were analysed SpectraSuite software using a Maya Pro 2000 spectrophotometer with SpectraSuite software. Maya Pro 2000 spectrophotometer
Chromophoric Dissolved Organic Matter Napierian Absorption Coefficients for Open Ocean Blue Water Samples from the North Atlantic Subtropical Gyre from R/V Atlantic Explorer AE1707, AE1829, AE1820, AE1905 at Bermuda Atlantic Time Series Stations (BATS) fChromophoric dissolved organic matter absorbance measurements were made from samples taken May 13, 2017 with an Agilent 8453 UV-visible spectrophotometer with ChemStation software.      Agilent 8453 UV-visible spectrophotometer
Metagenomic data from site U1382B at North Pond (IODP 336), western flank of the mid-Atlantic Ridge from core samples collected in November of 2011NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA)
Daily water chemistry measurements from a larval growth experiment culturing Olympia oysters in 50 unique combinations of temperature, salinity, and pCO2 over up to 17 days of larval life.Agilent 8453A UV-VUS diode array spectrophotometer
CO2 experiment physiology and carbonate chemistry from laboratory experiments with Pseudo-nitzschia australis conducted from 2021 to 2022Picarro cavity ring-down spectrophotometer
Total column ozone (O3) measured measured during DANCE cruise HRS1414 aboard the R/V Hugh R. Sharp from July to August 2014.Aerodyne CAPS; Thermo 49C; Thermo 48; Thermo 42C
Spectrophotometric pH measured at 25 degrees C using the total hydrogen ion scale from R/V Thomas G. Thompson and R/V Kilo Moana cruises TN277, KM1301, KM1312 in the Eastern North Pacific Ocean from 2012-2013 (POWOW project)Spectrophotometer
Spectrophotometric pH measured at the PICO time-series station (34.7181 deg N, 76.6707 deg W) from 2010 to 2012 (PICO project)Either a Cary 4000 or a Beckman DU 640 spectrophotomer was used. Spectrophotometer
Raw pH data acquired during the pH internal consistency experiment. Agilent 8454 spectrophotometer
Physiology of Montipora capitata and Porites compressa from October 2019 to September 2022 in response to marine heatwaves (2015 and 2019) in Hawaiʻihost-soluble protein concentration, chlorophyll a concentrations, total antioxidant capacity, melanin content, prophenoloxidase content, citrate synthase content: spectrophotometer (BioTek PowerWave XS2) BioTek PowerWave XS2
Phytoplankton cell counts at 3 pCO2 levels and 2 temperatures before and after 12 month conditioning off New Zealand South Island, near Taiaroa Head, 2011 (Plankton acclimation project)UV-vis spectrophotometer: Ocean Optics USB4000 spectrophotometer
Phytoplankton cell counts at 3 pCO2 levels and 2 temperatures before and after 12 month conditioning off New Zealand South Island, near Taiaroa Head, 2011 (Plankton acclimation project)Shimadzu 1800UV spectrophotometer
A U-3010 VIS Spectrophotometer was used to measure NH4+ concentration < 50 µM. U-3010 VIS Spectrophotometer
Cell abundance, growth rate, cellular P quotas, and alkaline phosphatase activity from a laboratory experiment examining the response of three species of marine phytoplankton grown under different phosphorus (P) conditionsGenesys 10 spectrophotometer
Absorbance at 3 wavelengths from pigment extractions of coral Orbicella faveolata from Rosaria and Varadero reef sites, Colombia, 2016 & 2017 (Varadero Reef project)mini spectrophotometer (Ocean Optics USB4000)
Pleurochrysis carterae diel culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project)Spectrophotometric pH measurements were taken of culture samples Hitachi U-3010 spectrophotometer
Pleurochrysis carterae growth cycle culture dynamics analyzed at Bigelow Laboratory from 2013 (OA Copes Coccoliths project)Spectrophotometric pH measurements were taken of culture samples Hitachi U-3010 spectrophotometer
Carbonate chemistry in experimental cultures of Plocamium cartilagineum cultured at different temperatures and pCO2 levels (Seaweed OA Resilience project)Used to measure pH (at temperature 25 C) Shimadzu UV-2450 UV-visible spectrophotometer
Carbonate chemistry over a time-course in pH drift experiments with Plocamium growth collected at Catalina Island, 2014-2015 (Seaweed OA Resilience project)Used to measure pH (at temperature 25 C) Shimadzu UV-2450 UV-visible spectrophotometer
Particulate organic matter (PON, POC, POP) concentrations collected on R/V Roger Revelle cruise RR1604 along the hydrographic line IO9 in the Eastern Indian Ocean from March to April 2016Genesys 10vis spectrophotometer (#840-208100, Thermo Scientific, Waltham, Massachusetts)  Genesys 10vis spectrophotometer
Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-15-18 at the Cape Lookout Continental Shelf and Slope during July 2015PO43- was measured spectrophotometrically. Spectrophotometer Thermofisher
Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-16-16 at the Cape Lookout Continental Shelf and Slope during May 2016PO₄³⁻ was measured spectrophotometrically Spectrophotometer Thermofisher
Sediment pore water and solid phase geochemical analyses from samples collected on R/V Savannah cruise SAV-17-15 in the Gulf of Mexico during July and August 2017NH₄⁺ was measured spectrophopotmetrically spectrophopotmeter
Concentrations of particulate phosphate in treatments amended with dissolved organic phosphate compounds in bioassay experiments (incubation 48h) with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019 Measurements of PP were performed using a conventional spectrophotometer (Genesys®,10 cm cuvette) after a high temperature combustion (450°C, 4.5h). Genesys®,10 cm cuvette
Concentrations of particulate phosphate in seawater collected during R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in the Spring of 2019PP samples were measured using a Gynesis spectrophotometer.   Gynesis spectrophotometer
Reef seawater biogeochemistry data from samples collected in the Jardines de la Reina reef-system, Cuba in November of 2017Acetone (90%) was used to extract Chlorophyll a and phaeophytin and the optical density (OD) values were measured on a calibrated spectrophotometer using standard optics (Lambda 18, Perkin Elmer, Waltham, MA, USA).   Lambda 18, Perkin Elmer, Waltham, MA, USA
Results from a study of physiological responses of Ulva lactuca to ocean acidification and nutrient enrichmentSoluble proteins, carbohydrates, and chl a were determined spectrophotometrically (Milton Roy Spectronic Genesys 5) Milton Roy Spectronic Genesys 5
CTD-associated variables, bottle salinity measurements, oxygen titrations, nutrient analyses, biogeochemical/biological variables, and DIC/Freon chemistry variables from R/V Roger Revelle cruise RR2004 along the 150W meridian from 30S to 60SHitachi U-3010 spectrophotometer (SN 0947-010)
Ammonium (NH4) and Nitrite (NO2) concentrations from R/V Roger Revelle cruise RR2311 in the Eastern Tropical South Pacific from November to December 2023GENESYS 150 UV-VIS Spectrophotometer
Discrete bottle sample measurements for carbonate chemistry, organic alkalinity and organic carbon from samples collected in Waquoit Bay and Vineyard Sound, MA in 2016Agilent 8454 UV-visible spectrophotometer
NCBI project accession and library information on each sample analyzed in upwelling experiments conducted on two phytoplankton species isolated from the California Upwelling ZoneRNA was purified and examined for quality and quantity using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Nanodrop 2000
Seawater nutrient/metabolite and flow cytometry metadata - Picoplankton incubation experiment 2020FDOM analysis: Horiba Aqualog scanning fluorometer with 150 W Xe excitation lamp, Peltier-cooled CCD emission detector, and a simultaneous absorbance spectrometer, Horiba Scientific, Piscataway, New Jersey, USA Absorbance spectrometer, Horiba Scientific, Piscataway, New Jersey, USA
Flow through sediment core incubations for nitrogen concentration and isotopic fluxes collected in 2013 on the Island of Sylt, Germany in the North Sea.Shimadzu UV 2550 Spectrophotometer
Sediment NO3 reduction rates, associated genes, and environmental data from bimonthly samples collected along the York River Estuary from June 2018 to April 2019Extracted chlorophyll-a was analyzed on a Beckman Coulter DU800 Spectrophotometer. Beckman Coulter DU800 Spectrophotometer
Sediment core porewater and particulate measurements from three sites on the Louisiana Shelf sampled during R/V Pelican cruises from December 2021 through August 2022Porewater was analyzed for dissolved si(OH)4 concentration using a spectrophotometric molybdate-blue method and a Thermo Scientific Genesys 10S UV-Vis Spectrophotometer. Thermo Scientific Genesys 10S UV-Vis Spectrophotometer
Sediment core porewater and particulate measurements from three sites on the Louisiana Shelf sampled during R/V Pelican cruises in August 2021Porewater was analyzed for dissolved si(OH)4 concentration using a spectrophotometric molybdate-blue method and a Thermo Scientific Genesys 10S UV-Vis Spectrophotometer. Thermo Scientific Genesys 10S UV-Vis Spectrophotometer
Carbon, Nitrogen, biogenic silica, thorium-234, and mass fluxes from upper ocean sediment traps at the Porcupine Abyssal Plain Sustained Observatory (PAP-SO) site in the Northeast Atlantic Ocean during RRS Discovery cruise DY077 in April of 2017Spectrophotometer
The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP) from UCSB MSI Passow Laboratory from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The pHT (total scale) was measured with a spectrophotometer (Shimadzu Biospec 16-1) using the indicator dye mcresol purple (Sigma-Aldrich) within 1–2 hours after sampling. spectrophotometer - Shimadzu Biospec 16-1
The effect of altered carbonate system chemistry on the abiotic formation of Transparent Exopolymer Particles (TEP).from UCSB MSI Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project) Spectrophotometer - Shimadzu Biospec 16-1
Series 1B-1: Multiple stressor experiments on T. pseudonana (CCMP1335) – pH, Dissolved Inorganic carbon (DIC), and Macronutrient concentrations in experimentsUsed for measurement of pH. Genesys 10SVIS
Series 1B-3: Multiple stressor experiments on T. pseudonana (CCMP1335) – computed data from the LC3 protocol for samples at 4 temperatures, 15-26CUsed for measurement of pH. Spectrophotometer (Genesys 10SVIS)
Aggregate Sinking Velocity from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
Aggregate Size from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
BioChemical results from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
Carbonate System results from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
General Information about the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
pH data from the CO2 Aggregation Experiment from UCSB Marine Science Institute Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The samples for pHT were collected bubble free in 20-ml scintillation vials and measured within 1-2 hours of sampling with a spectrophotometer (Genesys 10SVIS) equipped with a single cell Peltier (SPG1A, both Thermo Scientific) using the indicator dye m-cresol purple (Sigma-Aldrich) at a constant temperature of 25oC. Genesys 10SVIS Spectrophotometer
Series 2A-2: Multiple stressor experiments on T. pseudonana (CCMP1014) – photophysiologyUsed for measurement of pH. Spectrophotometer (Genesys 10SVIS)
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – cell abundance by flow cytometryUsed to measure pH. Genesys 10SVIS spectrophotometer
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - cell size estimated by forward scatter from flow cytometryUsed to measure pH. Genesys 10SVIS spectrophotometer
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) – pH measurementsUsed to measure sample pH. Genesys 10SVIS spectrophotometer
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - photophysiology measurementsUsed to measure pH. Genesys 10SVIS spectrophotometer
Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature, and bacteria - Aggregation Phase - Carbonate System + TEP from UCSB MSI Passow Lab from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The pH (total scale) was measured with a spectrophotometer using the indicator dye m-cresol purple (Sigma-Aldrich) within 1-2 hours of sampling at 25 oC (Clayton and Byrne 1993).   spectrophotometer
Aggregation of Thalassiosira weissflogii as a function of pCO2, temperature and bacteria - Aggregation Phase - Sinking Velocity from a from 2009 to 2010 (OA - Ocean Acidification and Aggregation project)The pH (total scale) was measured with a spectrophotometer using the indicator dye m-cresol purple (Sigma-Aldrich) within 1-2 hours of sampling at 25 oC (Clayton and Byrne 1993). spectrophotometer
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 – cell abundance and size by flow cytometryUsed to measure pH. Genesys 10SVIS spectrophotometer
Series 4A: Multiple stressor experiments on Synechococcus elongatus (CCMP1629) – pH measurementsUsed to measure sample pH. Genesys 10SVIS spectrophotometer
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - computed photophysiology parametersFor measurement of pH. Genesys 10SVIS
Series 4A: Multiple stressor experiments on the cyanobacteria Synechococcus elongatus CCMP1629 - raw fluorescence readings for photophysiology computationsFor measurement of pH. Genesys 10SVIS
Nutrients and iron in shipboard aerosol and rain samples collected during R/V Hugh R. Sharp cruise HRS1414 in the Mid-Atlantic Bight and northern South-Atlantic Bight from July to August of 2014 (DANCE project)Spectrofluorophotometer (NH4):  Shimadzu RF1501. Shimadzu RF1501
Size Fractionated Chlorophyll Measurements from R/V Tangaroa TAN1810 in the Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand) from October to November 2018 (Salp Food Web Ecology project)Chl and acidified phaeopigment-a concentrations were measured in the lab (within 3 months) using an ice-cold 90% acetone extraction by spectrofluorometric methods (APHA 10200 H) on a Varian Cary Eclipse fluorescence spectrophotometer (Rice et al., 2012). Varian Cary Eclipse fluorescence spectrophotometer
Oceanographic data from 12 monthly cruises off the Israeli coast in the east Mediterranean conducted between Feb 2018 and Jan 2019 at a continental shelf station (~140m depth) and an open-deep water station.NanoDrop 2000c (Thermo Sciences) NanoDrop 2000c (Thermo Sciences)
Programmable Flow Injection (pFI) Silicate Vertical Profile Data from R/V Investigator IN2023_V04 in the Southern Ocean during June 2023 (pFI-SI-LOV project)We used a miniSIA-2 instrument (miniSIA2, GlobalFIA, Fox Island, USA) equipped with a 8-port LOV unit and a 20 cm flow cell furnished with a Tungsten Halogen light source and UV-VIS spectrophotometer (Ocean Insight, USA) and a 14 position auto sampler (GlobalFIA, USA). UV-VIS spectrophotometer (Ocean Insight, USA)
CO2 analysis of water used for squid study from June 2011 (OA Squid Rearing project)Spectrophotometer
Experimental rearing of longfin squid under ocean acidification conditions from June 2011 (OA Squid Rearing project)Spectrophotometer
Concentrations of soluble reactive phosphate in treatments amended with dissolved organic phosphate compounds in bioassay experiments (incubation 48h) with seawater from R/V Savannah cruise SAV-19-02 in the NW Atlantic Ocean in Spring of 2019 Measurements of SRP were performed using the Liquid wave guide capillary cell (2.5 m length path, model 3250, World Precision Instrument) and using a conventional spectrophotometer (Genesys®,10 cm cuvette). Genesys®,10 cm cuvette
Stable isotopes in reactive silica pools of Mississippi River plume sediments collected aboard the R/V Pelican in May 2017Genesys 10S UV-Vis Spectrophotometer
CH4, H2, CO, and d13C concentrations from the Strytan Hydrothermal Field collected by SCUBA July 15-8 2012 (C-DEBI, Lost City-type hydrothermal system project) spectrophotometer at a wavelength of 670 nm
Dissolved inorganic carbon concentrations from the Strytan Hydrothermal Field collected by SCUBA July 15-8 2012 (C-DEBI, Lost City-type hydrothermal system project) spectrophotometer at a wavelength of 670 nm
Geochemistry measurements from the Strytan Hydrothermal Field collected by SCUBA July 15-8 2012 (C-DEBI, Lost City-type hydrothermal system project) spectrophotometer at a wavelength of 670 nm
Organic acid concentrations from the Strytan Hydrothermal Field collected by SCUBA July 15-8 2012 (C-DEBI, Lost City-type hydrothermal system project) spectrophotometer at a wavelength of 670 nm
Effects of temperature on biomass and sulfide production in Desulfovibrio hydrothermals (INSPIRE_Pyrite project)spectrophotometer
Accession numbers of symbiotic algae in coral collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)Nano-Drop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) Spectrophotometer
Laboratory results on symbiont type in recovering Porites divaricata corals collected from the Florida Keys, Bahamas, Panama, and Mexico during 2010 (SymBioSys project)Nano-Drop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) Spectrophotometer
Synechococcus batch culture data (cell quotas and ratios (C,N,P), size, and diameter) from laboratory experiments in 2021 to 2022 with related isolates cultured across a range of temperatures (16-25C) Genesys 10S UV-vis spectrophotometer (Thermo-Scientific)
Continuous culture studies of Synechococcus elongatus CCMP1629 in nitrate-limited and nutrient-replete cultures plus DNA results Used to measure pH Thermo Spectronic Heios spectrophotometer
Synthesis Product for Ocean Time Series (SPOTS) seapHox Ocean pH sensor (Satlantic/Sea-Bird Scientific) used by DYFAMED Ocean Optics S1000 used by CARIACO Spectrophotometer (not further specified) used by HOT, DYFAMED, RADCOR Combination electrode (not further specified) used by HOT Shimadzu UV-2401PC used by GIFT Spectrophotometer
T. rotula microbiome global sample
Continuous culture studies of possible climate change effects: Thalassiosira pseudonana CCMP1335 growth in nitrate-limited and nutrient-replete culturesUsed to measure pH Thermo Spectronic Heios spectrophotometer
Experimental results of nitrate-limited or nitrate-replete continuous culture studies of Thalassiosira pseudonana at 5 temperatures with either high or low pCO2 and irradianceUsed to measure pH Thermo Spectronic Heios spectrophotometer
Characteristics of tidepools from seasonal surveys conducted at tidepools along the California coast from 2017 to 2018
Transparent Exopolymer Particles (TEP) from CTD samples collected during R/V Hugh R. Sharp cruise HRS2204 from Apr to May 2022A spectrophotometer (Agilent 8453 UV-visible) was used to make the absorbance measurements, which were read using 1.0 cm polystyrene disposable cuvettes (Fisher-Scientific). Agilent 8453 UV-visible
Experimental results from a study of cellular quotas of carbon, nitrogen, and phosphorus in Trichodesmium erythraeum strain IMS101 under varying pCO2 and light conditions (PhytoTM_in_HighCO2 project)Residual P was estimated using the spectrophotometric method with a Barnstead/Turner SP-830 spectrophotometer at wavelength of 885 nm. Spectrophotometer
Discrete raw measurements from upwelling experiments conducted on two phytoplankton species isolated from the California Upwelling ZoneRNA was purified and examined for quality and quantity using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Nanodrop 2000
Bacteria, picoeukaryote, and Synecoccus cell counts, nutrients (POC, PON, POP) and chlorophyll from nitrate and vitamin-B enriched treatments, from up-welled coastal waters off Southern California, March 2015 (B-vitamin plankton succession project)Used to measure particulate organic phosphorus (POP).  Shimadzu UV-1800 UV/Visible Scanning Spectrophotometer
Physical and geochemical measurements of water and sediments measured from a UNCW 20-ft Center Console Boat in the Cape Fear River Estuary, NC in 2009 (Estuarine ANAMMOX project)Fe (II) and H2S were measured spectrophotometrically from porewater. Spectrophotometer
Water chemistry data collected from a laboratory experiment to investigate the interactive effects of temperature and pCO2 conditions on the behavior of sand dollar larvae (Dendraster excentricus)Used to measure pH.
Weekly coral calcification rates in the CO2 manipulation experiment conducted on Porites corals from PalauSpectrophotometric pH measurements were made with 2 mM m-Cresol purple indicator dye using a spectrometer with a 100 mm flow cell (Ocean Optics, mean precision = 0.005). Ocean Optics pH spectrophotometer
Inorganic nitrogen content; and nitrate, ammonium, and oxygen isotope composition of atmospheric particulates collected on Changdao Island, China from September 22, 2019 to September 5, 2020Discrete UV–Vis analyzer (Westco Smartchem 200): for ammonium, nitrate, and nitrite concentrations. Westco Smartchem 200
Total organic carbon and total nitrogen content; and nitrogen and oxygen isotope composition of precipitation collected at Qindao, Yantai and Changdao Island, China from May 17, 2019 to August 31, 2020.Discrete UV–Vis analyzer (Westco Smartchem 200): for ammonium, nitrate, and nitrite concentrations. Westco Smartchem 200
[Deprecated] Laboratory growth, photosynthetic, and respiration rates of Thalassiosira pseudonana clone 3H in nitrate limited culture (Stressors on Marine Phytoplankton project)Used to measure pH Thermo Spectronic Heios spectrophotometer
[Deprecated] Laboratory growth, photosynthetic, and growth rates of Thalassiosira pseudonana clone 3H in nitrate replete culture (Stressors on Marine Phytoplankton project)Used to measure pH Thermo Spectronic Heios spectrophotometer